Process for increasing the yield of Lactococcus lactis subsp, lactis and/or Lactococcus lactis subsp. cremoris bacteria cultures during aerobic fermentation

Patent metadata

Field	Value
Publication number	US-9854822-B2
Application number	US-81333806-A
Country	US
Kind code	B2
Filing date	Jan 5, 2006
Priority date	Jan 5, 2005
Publication date	Jan 2, 2018
Grant date	Jan 2, 2018

Abstract

Microbial starter cultures. More specifically, a method for preparing a microbial starter culture wherein the microorganism is inoculated in a culture medium comprising at least one 5 yield enhancing agent selected from the group consisting of a purine base, a pyrimidine base, a nucleoside and a nucleotide. Such microbial starter cultures are useful in the manufacturing of food, feed and pharmaceutical products.

First claim

The invention claimed is:

1. A method for obtaining harvested Lactococcus lactis subsp. lactis bacteria and/or Lactococcus lactis subsp. cremoris bacteria, comprising:
(i) culturing one or more of Lactococcus lactis subsp. lactis bacteria and Lactococcus lactis subsp. cremoris bacteria by aerating the culture in the presence of oxygen at conditions that allow the fermentation to proceed beyond an Optical Density measured at 600 nm (OD 600 ) of 10,
wherein said culture medium comprises:
yeast extract;
at least one porphyrin compound; and
at least one yield-enhancing agent at a concentration that is from 1 mM to 70 mM at the initiation of the fermentation and that is sufficient to yield at least 1 μM of said at least one yield-enhancing agent in the bacteria culture at the termination of the fermentation, wherein said at least one yield-enhancing agent is selected from the group consisting of inosine, inosinate (IMP), deoxyinosine, and deoxyinosinate (dIMP), wherein the OD 600 is at least 10 at the termination of the fermentation; and
(ii) harvesting said bacteria to obtain harvested Lactococcus lactis subsp. lactis bacteria and/or Lactococcus lactis subsp. cremoris bacteria.

2. The method according to claim 1 , wherein said culture medium comprises at least two yield-enhancing agents.

3. The method according to claim 2 , wherein two of said at least two yield-enhancing agents are inosine and IMP.

4. The method according to claim 1 , wherein said culture medium initially comprises an amount of said at least one yield-enhancing agent selected from the group consisting of 1 to 60 mM, 1.3 to 60 mM, 1.5 to 50 mM, 2 to 40 mM, 2.5 to 30 mM, 3 to 20 mM, 3 to 15 mM, 4 to 10 mM, and about 7 mM.

5. The method according to claim 1 , wherein the OD 600 of the culture medium at the termination of fermentation is selected from the group consisting of OD 600 =10 to OD 600 =200, OD 600 =15 to OD 600 =100, and OD 600 =20 to OD 600 =80.

6. The method according to claim 1 , wherein the Lactococcus lactis subsp. lactis bacteria and/or Lactococcus lactis subsp. cremoris bacteria are Lactococcus lactis subsp. cremoris bacteria.

7. The method according to claim 1 , wherein the Lactococcus lactis subsp. lactis bacteria and/or Lactococcus lactis subsp. cremoris bacteria are Lactococcus lactis subsp. lactis bacteria.

8. The method according to claim 1 , further comprising freezing said harvested Lactococcus lactis subsp. lactis bacteria and/or Lactococcus lactis subsp. cremoris bacteria to obtain frozen Lactococcus lactis subsp. lactis bacteria and/or Lactococcus lactis subsp. cremoris bacteria.

9. The method according to claim 8 , further comprising sublimating water from the frozen Lactococcus lactis subsp. lactis bacteria and/or Lactococcus lactis subsp. cremoris bacteria to obtain freeze-dried Lactococcus lactis subsp. lactis bacteria and/or Lactococcus lactis subsp. cremoris bacteria.

10. The method according to claim 8 , further comprising packing the frozen Lactococcus lactis subsp. lactis bacteria and/or Lactococcus lactis subsp. cremoris bacteria.

11. The method according to claim 8 , wherein at least one cryoprotectant is added to the harvested Lactococcus lactis subsp. lactis bacteria and/or Lactococcus lactis subsp, cremoris bacteria prior to freezing.

12. The method according to claim 1 , wherein the at least one yield-enhancing agent is selected from the group consisting of inosine and inosinate (IMP).

Assignees

• Kringelum Børge Windel
• Sørensen Niels Martin
• Garrigues Christel
• Pedersen Martin B
• Grøn Susanne
• Chr Hansen As

Inventors

• Kringelum Børge Windel
• Sørensen Niels Martin
• Garrigues Christel
• Pedersen Martin B
• Grøn Susanne

Classifications

• A23C9/123

  using only microorganisms of the genus lactobacteriaceae; Yoghurt (A23C9/13 takes precedence) · CPC title

• A23K10/18 Primary

  of live microorganisms · CPC title

• C12N1/38

  Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound (C12N1/34 takes precedence) · CPC title

• A23L33/135

  Bacteria or derivatives thereof, e.g. probiotics · CPC title

• A23L27/24

  prepared by fermentation · CPC title