Mass spectrometer arranged to perform MS/MS/MS

Patent metadata

Field	Value
Publication number	US-9852895-B2
Application number	US-201414445224-A
Country	US
Kind code	B2
Filing date	Jul 29, 2014
Priority date	Jan 21, 2009
Publication date	Dec 26, 2017
Grant date	Dec 26, 2017

Abstract

A mass spectrometer is disclosed comprising an on trap and a fragmentation device. Ions are fragmented in the ion trap to form first generation fragment ions. The ion trap has a relatively high mass cut-off. The first generation fragment ions are then transferred to a fragmentation device which is arranged to have a substantially lower low mass cut-off. The first generation fragment ions are fragmented within the fragmentation device any may optionally be stored in an ion accumulation region prior to being passed to a mass analyser for subsequent mass analysis.

First claim

The invention claimed is:

1. A method of mass spectrometry comprising:
fragmenting ions of interest within an ion trap to form a plurality of first fragment ions;
isolating and then fragmenting at least some of said first fragment ions within said ion trap to form a plurality of second fragment ions;
transferring at least some of said second fragment ions to a fragmentation device which is arranged either upstream or downstream of said ion trap;
fragmenting at least some said second fragment ions within said fragmentation device to form a plurality of third fragment ions.

2. A method as claimed in claim 1 , wherein said ion trap is operated in a mode of operation and has an effective first low mass or mass to charge ratio cut-off and wherein said fragmentation device is operated in a mode of operation and has an effective second low mass or mass to charge ratio cut-off, wherein said second low mass or mass to charge ratio cut-off is substantially lower than said first low mass or mass to charge ratio cut-off, wherein said first low mass or mass to charge ratio cut-off is defined as being a first mass or mass to charge ratio at which 50% of ions or less of a particular mass or mass to charge ratio remain confined within said ion trap for a particular period of time, and wherein said second low mass or mass to charge ratio cut-off is defined as being a second mass or mass to charge ratio at which 50% of ions or less of a particular mass or mass to charge ratio remain confined within said fragmentation device for said particular period of time.

3. A method as claimed in claim 1 , wherein said ion trap comprises a different number of electrodes or is structurally different to said fragmentation device so that for ions having a particular mass to charge ratio said ion trap has a first low mass cut-off and said fragmentation device has a second different low mass cut-off, wherein said first low mass or mass to charge ratio cut-off is defined as being a first mass or mass to charge ratio at which 50% of ions or less of a particular mass or mass to charge ratio remain confined within said ion trap for a particular period of time, and wherein said second low mass or mass to charge ratio cut-off is defined as being a second mass or mass to charge ratio at which 50% of ions or less of a particular mass or mass to charge ratio remain confined within said fragmentation device for said particular period of time.

4. A method as claimed in claim 1 , wherein said ion trap comprises a first plurality of electrodes having a first spacing or aperture size or diameter and wherein said fragmentation device comprises a second plurality of electrodes having a second different spacing or aperture size or diameter.

5. A method as claimed in claim 1 , further comprising:
accumulating at least some of said third fragment ions in an ion accumulation device or ion trap; and
releasing at least some of said third fragment ions from said ion accumulation device or ion trap and transferring said third fragment ions to a mass analyser for subsequent mass analysis.

6. A method as claimed in claim 1 , further comprising transferring said third fragment ions to a mass analyser for subsequent mass analysis.

7. A method as claimed in claim 1 wherein said ions of interest are:
precursor or parent ions of interest; or
fragment ions of interest.

8. A mass spectrometer comprising:
a control system, an ion trap and a fragmentation device arranged upstream or downstream of said ion trap;
wherein said control system of said mass spectrometer is arranged and adapted to fragment ions of interest within said ion trap to form a plurality of first fragment ions, wherein said control system of said mass spectrometer is arranged and adapted to isolate and then fragment at least some of said first fragment ions within said ion trap to form a plurality of second fragment ions, wherein said control system of said mass spectrometer is arranged and adapted to transfer at least some of said second fragment ions to said fragmentation device and wherein said control system of said mass spectrometer is arranged and adapted to fragment at least some of said second fragment ions within said fragmentation device to form a plurality of third fragment ions.

9. A mass spectrometer as claimed in claim 8 , wherein said ion trap is arranged and adapted to have an effective first low mass or mass to charge ratio cut-off and wherein said fragmentation device is arranged and adapted to have an effective second low mass or mass to charge ratio cut-off, wherein said second low mass or mass to charge ratio cut-off is substantially lower than said first low mass or mass to charge ratio cut-off, wherein said first low mass or mass to charge ratio cut-off is defined as being a first mass or mass to charge ratio at which 50% of ions or less of a particular mass or mass to charge ratio remain confined within said ion trap for a particular period of time, and wherein said second low mass or mass to charge ratio cut-off is defined as being a second mass or mass to charge ratio at which 50% of ions or less of a particular mass or mass to charge ratio remain confined within said fragmentation device for said particular period of time.

10. A mass spectrometer as claimed in claim 8 , wherein said ion trap comprises a different number of electrodes or is structurally different to said fragmentation device so that for ions having a particular mass to charge ratio said ion trap is arranged and adapted to have a first low mass cut-off and said fragmentation device is arranged and adapted to have a second different low mass cut-off, wherein said first low mass or mass to charge ratio cut-off is defined as being a first mass or mass to charge ratio at which 50% of ions or less of a particular mass or mass to charge ratio remain confined within said ion trap for a particular period of time, and wherein said second low mass or mass to charge ratio cut-off is defined as being a second mass or mass to charge ratio at which 50% of ions or less of a particular mass or mass to charge ratio remain confined within said fragmentation device for said particular period of time.

11. A mass spectrometer as claimed in claim 8 , wherein said ion trap comprises a first plurality of electrodes having a first spacing or aperture size or diameter and wherein said fragmentation device comprises a second plurality of electrodes having a second different spacing or aperture size or diameter.

12. A mass spectrometer as claimed in claim 8 , further comprising an ion accumulation device or ion trap arranged and adapted to accumulate at least some of said third fragment ions, wherein said control system of said mass spectrometer is arranged and adapted to release at least some of said third fragment ions from said ion accumulation device or ion trap and to transfer said at least some of said third fragment ions to a mass analyser for subsequent mass analysis.

13. A mass spectrometer as claimed in claim 8 , wherein said control system of said mass spectrometer is arranged and adapted to transfer said third fragment ions to a mass analyser for subsequent mass analysis.

14. A mass spectrometer as claimed in claim 8 wherein said ions of interest are:
precursor or parent ions of interest; or
fragment ions of interest.

Assignees

• Micromass Ltd

Inventors

• Kenny Daniel James

Classifications

• H01J49/0045 Primary

  characterised by the fragmentation or other specific reaction · CPC title

• H01J49/0031

  Step by step routines describing the use of the apparatus (H01J49/0081 takes precedence) · CPC title

• H01J49/06

  Electron- or ion-optical arrangements · CPC title

• H01J49/10

  Ion sources; Ion guns · CPC title

• H01J49/4225

  Multipole linear ion traps, e.g. quadrupoles, hexapoles · CPC title