Cathodized gold nanoparticle graphite pencil electrode and method for glucose detection

Patent metadata

Field	Value
Publication number	US-9851325-B2
Application number	US-201514719940-A
Country	US
Kind code	B2
Filing date	May 22, 2015
Priority date	Sep 30, 2013
Publication date	Dec 26, 2017
Grant date	Dec 26, 2017

Abstract

The cathodized gold nanoparticle graphite pencil electrode is a sensitive enzymeless electrochemical glucose sensor based on the cathodization of AuNP-GPE. Cyclic voltammetry shows that advantageously, the cathodized AuNP-GPE is able to oxidize glucose partially at low potential (around −0.27 V). Fructose and sucrose cannot be oxidized at <0.1 V, thus the glucose oxidation peak at around −0.27 V is suitable enough for selective detection of glucose in the presence of fructose and sucrose. However, the glucose oxidation peak current at around −0.27 V is much lower which should be enhanced to obtain low detection limit. The AuNP-GPE cathodization increases the oxidation peak current of glucose at around −0.27 V. The dynamic range of the sensor is in the range between 0.05 to 5.0 mM of glucose with good linearity (R 2 =0.999). Almost no interference effect was observed for sensing of glucose in the presence of fructose, sucrose and NaCl.

First claim

We claim:

1. A method for forming a cathodized gold nanoparticle graphite electrode, comprising the steps of:
placing a solid graphite member into a tube filled with a prepared solution of L-ascorbic acid (AA) and Gold(III) chloride;
placing the filled tube and solid graphite member combination into a water bath preheated to approximately 75° centigrade (C.);
retaining said filled tube and solid graphite member combination in said water bath for approximately 15 minutes to form a gold nanoparticle graphite electrode (AuNP-GPE);
removing said solid graphite member from said tube, said solid graphite member being transformed into said AuNP-GPE;
washing said AuNP-GPE in deionized water;
drying said AuNP-GPE at approximately 60° C. for approximately 5 minutes prior to use; and
cathodizing said AuNP-GPE by placing said AuNP-GPE in a basic solution and applying −1.0 volts to said AuNP-GPE for approximately 30 seconds.

2. The method for forming a cathodized gold nanoparticle graphite electrode according to claim 1 , wherein said basic solution is a glucose analyte solution.

3. The method for forming a cathodized gold nanoparticle graphite electrode according to claim 1 , wherein said basic solution consists essentially of NaOH.

4. The method for forming a cathodized gold nanoparticle graphite electrode according to claim 1 , wherein said basic solution includes NaOH and glucose.

5. The method for forming a cathodized gold nanoparticle graphite electrode according to claim 1 , wherein said basic solution consists essentially of a 0.1 molar (M) NaOH including glucose.

6. The method for forming a cathodized gold nanoparticle graphite electrode according to claim 1 , further comprising the step of:
preparing said AA and Gold (III) chloride solution in equal volumes, approximately 1.5 milliliters (ml) of each aqueous solutions of approximately 1.65 millimolar (mM) AA and 1.0 mM Gold(III) chloride, wherein said tube is approximately 3.0 ml in volume.

7. The method for forming a cathodized gold nanoparticle graphite electrode according to claim 1 , wherein said solid graphite member is a mechanical pencil lead.

8. The method for forming a cathodized gold nanoparticle graphite electrode according to claim 7 , wherein said mechanical pencil lead is a hi-polymer graphite pencil HB grade lead.

9. The method for forming a cathodized gold nanoparticle graphite electrode according to claim 7 , further comprising the step of:
fixing an electrically conductive holder of said mechanical pencil lead vertically with 15 millimeters (mm) of said mechanical pencil lead extruded outside said electrically conductive holder and 10 mm of said mechanical pencil lead immersed in a glucose analyte solution as said basic solution, maintaining a geometric electrode area of approximately 15.90 mm 2 .

10. The method for forming a cathodized gold nanoparticle graphite electrode according to claim 1 , wherein placement of said solid graphite member into the tube of AA and Gold(III) chloride chemically deposits gold nanoparticles in the range of 20-85 nanometers (nm) on said solid graphite member.

Assignees

• Univ King Fahd Pet & Minerals
• King Abdulaziz City Sci & Tech

Inventors

• Kawde Abdel-Nasser Metwally Aly
• Aziz Md Abdul

Classifications

• G01N27/3277

  being a redox reaction, e.g. detection by cyclic voltammetry (voltammetry per se G01N27/42, G01N27/48) · CPC title

• G01N27/30

  Electrodes, e.g. test electrodes; Half-cells (G01N27/414 takes precedence) · CPC title

• G01N27/327 Primary

  Biochemical electrodes {, e.g. electrical or mechanical details for in vitro measurements} · CPC title

• G01N27/3278 Primary

  involving nanosized elements, e.g. nanogaps or nanoparticles (nanopores G01N33/48721; magnetic beads G01N27/745) · CPC title

• G01N27/48

  using polarography, i.e. measuring changes in current under a slowly-varying voltage · CPC title