Device and methods for detection of analytes including use of a colorimetric barcode

Patent metadata

Field	Value
Publication number	US-9851307-B2
Application number	US-201314651500-A
Country	US
Kind code	B2
Filing date	Dec 18, 2013
Priority date	Dec 20, 2012
Publication date	Dec 26, 2017
Grant date	Dec 26, 2017

Abstract

Embodiments described herein related to devices and methods for the collection and/or determination of analytes, such as illicit substances including military explosives, explosives, and precursors thereof. In some cases, the device may be a disposable device that incorporates highly efficient sample collection in combination with microfluidic-based chemical analysis resulting in the rapid detection and identification of unknown materials. In some cases, multiple colorimetric detection chemistries may be employed, and the resulting “barcode” of color changes can be used to positively identify the presence and/or identity of the analyte.

First claim

What is claimed:

1. A method for determining an analyte, comprising:
introducing a sample suspected of containing an analyte into a sample inlet of a sample analysis device, the sample analysis device comprising a plurality of channels constructed and arranged to receive the sample suspected of containing an analyte from the sample inlet, each channel comprising a different chemical reagent, each chemical reagent capable of generating a luminescent or colorimetric signal;
treating the sample with a plurality of different solvents provided from a corresponding plurality of solvent chambers disposed within the sample analysis device, wherein the plurality of solvent chambers are in fluid communication with the sample inlet; and
simultaneously introducing the treated sample into the plurality of channels such that the analyte, if present, interacts with one or more of the chemical reagents to produce a plurality of luminescent or colorimetric signals, thereby determining the analyte, wherein the plurality of channels are in fluid communication with the sample inlet, and
wherein the presence and/or identity of the analyte is determined without need for a non-luminescent or non-colorimetric analysis of the sample.

2. A method as in claim 1 , wherein the chemical reagents are in substantially solid form.

3. A method as in claim 1 , wherein the sample analysis device comprises:
at least five channels; and
a sample collection pad with a gas permeable filter configured to receive and filter the sample at the sample inlet;
a mixing chamber located downstream from and in fluid communication with the sample inlet,
wherein the plurality of solvent chambers comprise at least three blister buttons disposed within the sample analysis device and in fluid communication with the mixing chamber to provide at least three different solvents sequentially to treat the sample at the mixing chamber before the sample is introduced into the at least five channels.

4. A method as in claim 1 , wherein at least one channel comprises a reaction well containing the chemical reagent, wherein at least a portion of the sample contacts the chemical reagent.

5. A method as in claim 1 , wherein at least one channel comprises a first reaction well containing a pre-treatment reagent and a second reaction well containing a chemical reagent, the second well positioned downstream from the first reaction well, wherein at least a portion of the sample first contacts the pre-treatment reagent and then subsequently contacts the chemical reagent.

6. A method as in claim 1 , wherein at least one channel comprises a first reaction well containing a first chemical reagent and a second reaction well containing a second chemical reagent, the second well positioned downstream from the first reaction well, wherein at least a portion of the sample first contacts the first chemical reagent and then subsequently contacts the second chemical reagent.

7. A method as in claim 1 , wherein at least one channel comprises a mixing region positioned downstream from a reaction well containing the chemical reagent, such that a mixture comprising the sample and the chemical reagent are allowed to react to produce a luminescent or colorimetric signal.

8. A method as in claim 1 , wherein at least one channel comprises a detection well positioned downstream from a mixing region, wherein the luminescent or colorimetric signal is determined within the detection well.

9. A method as in claim 1 , wherein the analyte is a drug, chemical warfare agent, biological warfare agent, toxic industrial chemical, or toxic industrial metal.

10. A method as in claim 1 , wherein the analyte is gun powder or gunshot residue, TNT, DNT, tetryl, nitroglycerin, EGDN, PETN, RDX, HMX, nitrate, chlorate, perchlorate, permanganate, peroxide-based explosive, Al, Mg, or a precursor thereof.

11. A method as in claim 1 , wherein the analyte is present in water.

12. A method as in claim 1 , wherein the chemical reagent comprises a reagent capable of forming a Meisenheimer complex, a reagent capable of undergoing a Griess reaction, p-DMAC, Berthlot's reagent, aniline sulfate, [Pt(tpy)Cl]PF 6 , a boronate-containing species, diphenylamine, aluminon, diaminobenzene, a pyrocatechol violet reagent, or a nitrophenylazoresorcinal reagent.

13. A method as in claim 1 , wherein the sample analysis device is free of a non-luminescent reagent or non-colorimetric reagent.

14. A method as in claim 1 , wherein the sample analysis device does not comprise a crystallizing agent.

15. A method as in claim 1 , wherein the sample is collected by contacting a surface of a sample collection device with an article suspected of containing the analyte.

16. A method as in claim 15 , further comprising:
contacting the surface of the sample collection device with a fluid carrier provided in the sample collection device to produce a fluid sample containing the analyte, if present.

17. A method as in claim 16 , wherein the fluid carrier comprises an organic solvent.

18. A method as in claim 15 , further comprising:
contacting the surface of the sample collection device with a fluid carrier provided in the sample collection device to produce a fluid sample containing the analyte, if present, wherein the fluid sample is introduced to the sample analysis device.

19. A method as in claim 15 , wherein the surface is a substantially planar surface.

20. A method as in claim 15 , wherein the surface is the surface of a rolling substrate.

21. A method as in claim 20 , wherein the rolling substrate comprises an adhesive material.

22. A method as in claim 15 , wherein the sample collection device and the sample analysis device are provided together in a kit.

23. A method for determining an analyte, comprising:
contacting a surface of a rolling substrate of a sample collection device with an article suspected of containing an analyte via a rolling or tapping motion such that a sample suspected of containing the analyte is collected on the surface of the rolling substrate;
contacting the surface of the rolling substrate with a fluid carrier to transfer at least a portion of the sample to the fluid carrier to produce a fluid sample suspected of containing the analyte;
introducing the fluid sample suspected of containing the analyte into a sample inlet of a sample analysis device comprising a plurality of channels constructed and arranged to receive the fluid sample;
treating the fluid sample with a plurality of solvents provided from a corresponding plurality of solvent chambers disposed within the sample analysis device, wherein the plurality of solvent chambers are in fluid communication with the sample inlet;
simultaneously introducing portions of the fluid sample into each of a set of the plurality of channels, each of the set of channels comprising a different chemical reagent capable of generating a luminescent and/or colorimetric signal upon interaction with a specific analyte, wherein the analyte, if present in the fluid sample, interacts with one or more of the chemical reagents to produce a plurality of luminescent and/or colorimetric signals in at least some of the set of channels, wherein the plurality of channels are in fluid communication with the sample inlet; and
determining the presence and/or identity of the analyte based on the plurality of luminescent and/or colorimetric signals.

24. A kit for determining an analyte, comprising:
a sample collection devi

Assignees

• Flir Detection Inc

Inventors

• Deans Robert
• Loges Peter G
• Mcdaniel William
• Paul Kateri E
• Hancock Lawrence F
• Szabo Matthew Joseph

Classifications

• B01L2300/0681

  Filter · CPC title

• B01L3/5023

  with a sample being transported to, and subsequently stored in an absorbent for analysis · CPC title

• Y10T436/2575

  Volumetric liquid transfer · CPC title

• B01L2300/087

  Multiple sequential chambers · CPC title

• G01N21/66

  electrically excited, e.g. electroluminescence · CPC title