Yeast cells having reductive TCA pathway from pyruvate to succinate and overexpressing an exogenous NAD(P)+ transhydrogenase enzyme

Patent metadata

Field	Value
Publication number	US-9850507-B2
Application number	US-201314416633-A
Country	US
Kind code	B2
Filing date	Jul 25, 2013
Priority date	Jul 25, 2012
Publication date	Dec 26, 2017
Grant date	Dec 26, 2017

Abstract

Yeast cells having a reductive TCA pathway from pyruvate or phosphoenolpyruvate to succinate, and which include at least one exogenous gene overexpressing an enzyme in that pathway, further contain an exogenous transhydrogenase gene.

First claim

The invention claimed is:

1. A recombinant yeast cell engineered to produce succinate through an active reductive tricarboxylic acid (TCA) pathway from pyruvate or phosphoenolpyruvate to succinate, wherein the recombinant yeast cell is modified from a parent yeast cell by having integrated into its genome an exogenous gene encoding a soluble nicotinamide adenine dinucleotide phosphate (NAD(P)+) transhydrogenase enzyme, wherein the soluble NAD(P)+transhydrogenase enzyme is expressed in the cytosol of the recombinant yeast cell, wherein the recombinant yeast cell is further modified from the parent yeast cell by having integrated into its genome at least one of:
(i) an exogenous pyruvate carboxylase gene that encodes an enzyme which catalyzes the conversion of pyruvate to oxaloacetate;
(ii) an exogenous malate dehydrogenase gene which encodes an enzyme that catalyzes the conversion of oxaloacetate to malate;
(iii) an exogenous fumarase gene that encodes an enzyme which catalyzes the conversion of malate to fumarate; and
(iv) an exogenous fumarate reductase gene which encodes an enzyme which catalyzes the conversion of fumarate to succinate, and wherein the recombinant yeast cell produces more succinate through the active reductive TCA pathway as compared to the parent cell.

2. The recombinant yeast cell of claim 1 , wherein the recombinant yeast cell is further modified from the parent yeast cell by having integrated into its genome at least one non-native malate dehydrogenase gene which encodes an enzyme that catalyzes the conversion of oxaloacetate to malate.

3. The recombinant yeast cell of claim 1 , wherein the recombinant yeast cell is further modified from the parent yeast cell by having integrated into its genome at least one non-native fumarate reductase gene which encodes an enzyme which catalyzes the conversion of fumarate to succinate.

4. The recombinant yeast cell of claim 1 wherein the recombinant yeast cell is further modified from the parent yeast cell by having integrated into its genome at least one non-native 6-phosphogluconate dehydrogenase gene that encodes a 6-phosphogluconate dehydrogenase enzyme.

5. The recombinant yeast cell of claim 1 , wherein the recombinant yeast cell is further modified from the parent yeast cell by having integrated into its genome at least one non-native glucose 6-phosphate dehydrogenase gene that encodes a glucose 6-phosphate dehydrogenase enzyme.

6. The recombinant yeast cell of claim 1 , wherein the recombinant yeast cell is further modified from the parent yeast cell by having integrated into its genome at least one non-native Stb5p gene that encodes an Stb5p protein.

7. The recombinant yeast cell of claim 1 , wherein the recombinant yeast cell is further modified from the parent yeast cell by having a deletion or disruption of a native phosphoglucose isomerase gene.

8. The recombinant yeast cell of claim 1 , wherein the recombinant yeast cell is further modified from the parent yeast cell by having a deletion or disruption of a native pyruvate decarboxylase gene.

9. The recombinant yeast cell of claim 1 , wherein the recombinant yeast cell is a yeast of the species Issatchenkia orientalis.

10. The recombinant yeast cell of claim 1 , wherein the recombinant yeast cell is a yeast of the species: Candida sonorensis, Kluyveromyces marxianus, Kluyveromyces thermotolerana, Candida methanesobosa, Issatchenkia orientalis, Candida lambica, Candida sorboxylosa, Candida zemplinina, Candida geochares, Pichia membranifaciens, Zygosaccharomyces kombuchaensis, Candida sorbosivorans, Candida vanderwaltii, Candida sorbophila, Zygosaccharomyces bisporus, Zygosaccharomyces lentus, Saccharomyces bayanus, Candida boidinii, Candida etchellsii, Kluyvermyces lactis, Pichia jadinii , or Pichia anomala.

11. The recombinant yeast cell of claim 1 , wherein the recombinant yeast cell produces no more than 2% of the theoretical yield of ethanol.

12. The recombinant yeast cell of claim 1 , wherein the recombinant yeast cell exhibits a volumetric glucose consumption rate of at least 0.9 gram of glucose per liter of broth per hour when cultivated at 30° C. with shaking at 150 rpm for 96 hours in a 250 mL baffled shake flask containing 1.28 g/L dry CaCO 3 , 50 mL of shake flask medium which is a sterilized, 4.5 pH aqueous solution of urea (2.3 g/L), magnesium sulfate heptahydrate (0.5 g/L), potassium phosphate monobasic (3.0 g/L), trace element solution (1 mL/L), vitamin solution (1 mL/L), glucose (120.0 g/L), glycerol (0.1 g/L), and 2-(N-Morpholino) ethanesulfonic acid (MES) (4.0 g/L), wherein the trace element solution is an aqueous solution of EDTA (15.0 g/L), zinc sulfate heptahydrate (4.5 g/L), manganese chloride dehydrate (1.0 g/L), cobalt(II) chloride hexahydrate (0.3 g/L), copper(II)sulfate pentahydrate (0.3 g/L), disodium molybdenum dehydrate (0.4 g/L), calcium chloride dehydrate (4.5 g/L), iron sulphate heptahydrate (3 g/L), boric acid (1.0 g/L), and potassium iodide (0.1 g/L), and wherein the vitamin solution is an aqueous solution of biotin (D-; 0.05 g/L), calcium pantothenate (D+; 1 g/L), nicotinic acid (5 g/L), myo-inositol (25 g/L), pyridoxine hydrochloride (1 g/L), and paminobenzoic acid (0.2 g/L).

13. The recombinant yeast cell of claim 1 , wherein the recombinant yeast cell is further modified from the parent yeast cell by having a deletion or disruption of an endogenous glycerol-3-phosphate dehydrogenase gene.

14. The recombinant yeast cell of claim 1 , wherein the recombinant yeast cell has no deletion or disruption of an endogenous phosphoglucose isomerase gene and produces a native phosphoglucose isomerase enzyme.

15. The recombinant yeast cell of claim 14 , wherein the recombinant yeast cell is a yeast of the species Issatchenkia orientalis.

16. The recombinant yeast cell of claim 1 , wherein the recombinant yeast cell comprises integrated into its genome at least two of (i) to (iv).

17. The recombinant yeast cell of claim 1 , wherein the recombinant yeast cell comprises integrated into its genome at least three of (i) to (iv).

18. The recombinant yeast cell of claim 1 , wherein the recombinant yeast cell comprises integrated into its genome all of (i) to (iv).

Assignees

• Cargill Inc
• Bioamber Inc

Inventors

• Rush Brian J
• Watts Kevin T
• Mcintosh Jr Vernon L
• Fosmer Arlene M
• Poynter Gregory M
• Mcmullin Thomas W

Classifications

• C12Y604/01001

  Pyruvate carboxylase (6.4.1.1) · CPC title

• C12N9/0006

  acting on CH-OH groups as donors (1.1) · CPC title

• Y02E50/343

  Cross-Sectional Technologies · mapped topic

• C12P7/18

  polyhydric · CPC title

• C12N15/52

  Genes encoding for enzymes or proenzymes · CPC title