Genes encoding novel lipid transporters and their use to increase oil production in vegetative tissues of plants

Patent metadata

Field	Value
Publication number	US-9850291-B2
Application number	US-201514719486-A
Country	US
Kind code	B2
Filing date	May 22, 2015
Priority date	May 23, 2014
Publication date	Dec 26, 2017
Grant date	Dec 26, 2017

Abstract

The present invention discloses a novel gene encoding a transporter protein trigalactosyldiacylglycerol-5 (TGD5), mutations thereof and their use to enhance TAG production and retention in plant vegetative tissue.

First claim

The invention claimed is:

1. A composition comprising the nucleotide sequence SEQ ID NO: 3.

2. A composition comprising the amino acid sequence SEQ ID NO: 4.

3. A composition comprising the nucleotide sequence SEQ ID NO: 3 or the amino acid sequence SEQ ID NO: 4 that results in diminished trigalactosyldiacylglycerol-5 (TGD5) protein function or activity as compared to an otherwise identical plant.

4. The composition of claim 3 wherein said composition is a plant, progeny having the same genotype as the plant, or seeds having the same genotype as the plant.

5. The plant of claim 4 further comprising an increased copy number selected from the group consisting of genes encoding phospholipid: diacylglycerol acyltransferase (PDAT), genes encoding oleosin (OLE), genes encoding WRINKLED1 (WRI1), and combinations thereof.

6. The plant of claim 5 further comprising an increased copy number of genes encoding phospholipid:diacylglycerol acyltransferase (PDAT), and an increased copy number of genes encoding oleosin (OLE), and an increased copy number of genes encoding WRINKLED1 (WRI1).

7. The plant of claim 4 further comprising a defective sugar-dependent 1 TAG lipase gene (sdpl).

8. The plant of claim 4 wherein diminished amounts of wild type TGD5 as compared to wild type amounts of wild type TGD5 are expressed.

9. A method for increasing production and retention of oil in vegetative tissues of a plant, the method comprising: providing a plant comprising: a) the nucleotide sequence SEQ ID NO: 3 or the amino acid sequence SEQ ID NO: 4, and further comprising b) increased expression levels of a gene encoding phospholipid:diacylglycerol acyltransferase (PDAT), and c) increased expression levels of a gene encoding oleosin (OLE); and growing said plant to accumulate oil in vegetative tissues of said plant.

10. The method of claim 9 , additionally comprising extracting said oil from said plant.

11. A method for enhancing the caloric content of vegetative tissue of a plant comprising: providing a plant comprising the nucleotide sequence SEQ ID NO: 3 or the amino acid sequence SEQ ID NO: 4; and growing said plant to accumulate in vegetative tissues of said plant.

12. A method for enhancing the caloric content of vegetative tissue of a plant comprising: providing a plant comprising the nucleotide sequence SEQ ID NO: 3 or the amino acid sequence SEQ ID NO: 4 and further comprising a defective sugar-dependent1 TAG lipase (sdp1) gene; and growing said plant to accumulate oil in vegetative tissues of said plant.

Assignees

• Brookhaven Science Ass Llc

Inventors

• Xu Changcheng
• Fan Jilian
• Yan Chengshi
• Shanklin John

Classifications

• C07K14/415 Primary

  from plants · CPC title

• C12N15/8247

  involving modified lipid metabolism, e.g. seed oil composition · CPC title