Microfluidic device

Patent metadata

Field	Value
Publication number	US-9849436-B2
Application number	US-201414909796-A
Country	US
Kind code	B2
Filing date	Mar 10, 2014
Priority date	Aug 8, 2013
Publication date	Dec 26, 2017
Grant date	Dec 26, 2017

Abstract

A microfluidic device includes a channel through which a reaction solution flows. The channel passes through a reaction section having a plurality of temperature zones set at predetermined different temperatures. The channel includes, at least in the reaction section, a region where a cross-sectional area decreases in a feeding direction of the reaction solution.

First claim

What is claimed is:

1. A microfluidic device comprising:
a channel through which a reaction solution flows, the channel passing through a reaction section having a plurality of temperature zones set at predetermined different temperatures,
wherein an effective cross-sectional area of the channel across the entirety of the reaction section gradually decreases in a feeding direction of the reaction solution.

2. The microfluidic device according to claim 1 , wherein
the channel in the reaction section has a tapered structure.

3. The microfluidic device according to claim 2 , wherein
the channel in the reaction section has a tapered width and a constant depth.

4. The microfluidic device according to claim 1 , wherein
the effective cross-sectional area of the channel in the reaction section decreases in a step-wise fashion in the feeding direction of the reaction solution.

5. The microfluidic device according to claim 1 , wherein
the effective cross-sectional area of the channel in the reaction section is gradually decreased via a plurality of pillars disposed in the channel.

6. The microfluidic device according to claim 1 , wherein
the channel is a meandering channel arranged to pass back and forth through the plurality of temperature zones, and
the reaction solution is subjected to cyclic temperature changes by being fed through the meandering channel.

7. The microfluidic device according to claim 6 , further comprising the reaction solution, wherein
the reaction solution includes a target nucleic acid, and
the target nucleic acid is amplified by a polymerase chain reaction as a result of the reaction solution passing through the reaction section of the channel.

8. The microfluidic device according to claim 1 , further comprising the reaction solution, wherein
the reaction solution includes one of a bacteria and virus as an analyte, and
the microfluidic device detects the analyte included in the reaction solution.

9. The microfluidic device according to claim 8 , wherein
an antibody that specifically reacts with the analyte is immobilized in the channel.

10. The microfluidic device according to claim 1 , wherein
a portion of the channel is divided into branches.

11. The microfluidic device according to claim 1 , wherein
the channel is formed in a substrate, and
the substrate includes one of silicon, resin, and glass.

12. The microfluidic device according to claim 1 , wherein
the effective cross-sectional area of the channel in the reaction section monotonically decreases.

13. A microfluidic device comprising:
a channel through which a reaction solution flows, the channel passing through a reaction section having a plurality of temperature zones set at predetermined different temperatures, wherein
an effective cross-sectional area of the channel in the reaction section gradually decreases in a feeding direction of the reaction solution such that the cross-sectional area of the channel in the reaction section maintains the reaction solution at a constant velocity as the reaction solution passes through each of the plurality of temperature zones of the reaction section,
the effective cross-sectional area of the channel in the reaction section decreases in a step-wise fashion in the feeding direction of the reaction solution,
the reaction section includes a plurality of lines comprising sections of the channel having different orientations with respect to each other that are arranged in a meandering fashion though the plurality of temperature zones, and
the effective cross-sectional area of the channel in the reaction section decreases in the step-wise fashion with each line in the feeding direction.

14. The microfluidic device according to claim 13 , wherein
the channel in the reaction section has a narrower width with each line and a constant depth.

15. A microfluidic device comprising:
a channel through which a reaction solution flows, the channel passing through a reaction section having a plurality of temperature zones set at predetermined different temperatures, wherein
the channel includes, at least in the reaction section, a region where a cross-sectional area decreases in a feeding direction of the reaction solution,
the channel includes, in the reaction section, a region where a cross-sectional area decreases in a step-wise fashion in the feeding direction of the reaction solution,
in the region where the cross-sectional area decreases in a step-wise fashion, the channel includes a plurality of lines comprising sections of the channel having different orientations with respect to each other that are arranged in a meandering fashion through the plurality of temperature zones, and
in the region where the cross-sectional area decreases in a step-wise fashion, the cross-sectional area of the channel decreases with each line in the feeding direction.

Assignees

• Panasonic Corp

Inventors

• Tachibana Hiroaki
• Tamiya Eiichi
• Saito Masato

Classifications

• B01J2219/0086

  Dimensions of the flow channels · CPC title

• B01J2219/0084

  For changing surface tension · CPC title

• B01J2219/00873

  Heat exchange · CPC title

• B01L2300/0883

  Serpentine channels · CPC title

• B01J2219/00995

  Mathematical modeling · CPC title