Storage stable frozen lactic acid bacteria culture

Patent metadata

Field	Value
Publication number	US-9848615-B2
Application number	US-201414587953-A
Country	US
Kind code	B2
Filing date	Dec 31, 2014
Priority date	Jan 22, 2003
Publication date	Dec 26, 2017
Grant date	Dec 26, 2017

Abstract

A storage stable frozen lactic acid bacteria (LAB) culture that comprises LAB that are that are able to utilize sucrose, has a weight of at least 50 g frozen material and a content of viable bacteria of at least 10 9 colony forming units (CFU) per g frozen material.

First claim

The invention claimed is:

1. A frozen lactic acid bacteria culture comprising:
a mixture of mesophilic lactic acid bacteria that (a) have an optimum growth temperature of about 30° C. and (b) are capable of utilizing sucrose, and
from 0.5% to 80% w/w of at least one cryoprotective agent,
wherein the frozen culture is not freeze-dried and has a weight of at least 50 grams and comprises at least 10 9 colony forming units of viable bacteria per gram.

2. The frozen culture of claim 1 , wherein the culture is a LD-culture comprising Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. cremoris, Lactococcus lactis subsp. lactis biovar. diacetylactis and Leuconostoc mesenteroides subsp. cremoris.

3. The frozen culture of claim 1 , wherein the culture is an L-culture.

4. The frozen culture of claim 1 , further comprising a thermophilic Streptococcus thermophilus bacterium.

5. The frozen culture of claim 1 , further comprising a thermophilic Lactobacillus acidophilus bacterium.

6. The frozen culture of claim 1 , wherein the at least one cryoprotective agent comprises a carbohydrate.

7. The frozen culture of claim 1 , wherein the at least one cryoprotective agent comprises a disaccharide.

8. The frozen culture of claim 1 , wherein the at least one cryoprotective agent comprises at least one of trehalose and sucrose.

9. The frozen culture of claim 1 , wherein the at least one cryoprotective agent comprises a mixture of a disaccharide and a polysaccharide.

10. The frozen culture of claim 9 , wherein the disaccharide comprises sucrose and the polysaccharide comprises maltodextrin.

11. The frozen culture of claim 1 , wherein the at least one cryoprotective agent is selected from the group consisting of a protein, a protein hydrolysate, and an amino acid.

12. The frozen culture of claim 1 , wherein the frozen culture comprises from 3% to 50% w/w of the at least one cryoprotective agent.

13. The frozen culture of claim 1 , wherein the frozen culture comprises from 4% to 10% w/w of the at least one cryoprotective agent.

14. A food or feed product made using the frozen culture of claim 1 .

15. A method of making a food or feed product, wherein the method comprises adding the frozen culture of claim 1 to a food or feed material.

16. A method for making a frozen lactic acid bacteria culture comprising:
(i) adding at least one cryoprotective agent to a mixture of mesophilic lactic acid bacteria that (a) have an optimum growth temperature of about 30° C. and (b) are capable of utilizing sucrose; and then
(ii) freezing the culture,
wherein the frozen culture is not freeze-dried and has a weight of at least 50 grams and comprises (a) at least 10 9 colony forming units viable bacteria per gram and (b) from 0.5% to 80% w/w of the at least one cryoprotective agent.

17. The method of claim 16 , wherein the culture is a LD-culture comprising Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. cremoris, Lactococcus lactis subsp. lactis biovar. diacetylactis and Leuconostoc mesenteroides subsp. cremoris.

18. The method of claim 16 , wherein the frozen mixture comprises from 3% to 50% w/w of the at least one cryoprotective agent.

19. The method of claim 16 , wherein the frozen culture comprises from 4% to 10% w/w of the at least one cryoprotective agent.

Assignees

• Chr Hansen As

Inventors

• Bisgaard-Frantzen Hans
• Kringelum Børge Windel
• Knap Inge

Classifications

• C12N1/00 Primary

  Microorganisms; Compositions thereof (medicinal preparations containing material from protozoa, bacteria or viruses A61K35/66, from algae A61K36/02, from fungi A61K36/06; preparing medicinal bacterial antigen or antibody compositions, e.g. bacterial vaccines, A61K39/00); Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor · CPC title

• A23C9/123 Primary

  using only microorganisms of the genus lactobacteriaceae; Yoghurt (A23C9/13 takes precedence) · CPC title

• C12N1/04

  Preserving or maintaining viable microorganisms (immobilised microorganisms C12N11/00) · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title

• A23Y2240/25

  Human Necessities · mapped topic