Optical method and device for identifying and quantifying analytes

Patent metadata

Field	Value
Publication number	US-9846154-B2
Application number	US-201214366597-A
Country	US
Kind code	B2
Filing date	Dec 20, 2012
Priority date	Dec 20, 2011
Publication date	Dec 19, 2017
Grant date	Dec 19, 2017

Abstract

A method for analyzing a metalloprotein and/or the interaction with its environment comprising the following steps: (a) Providing a medium that enhances the detection of the electromagnetic cross-section signal of metalloproteins, (b) Incorporating a metalloprotein to analyse into said medium, (c) Contacting said medium with electromagnetic radiation, (d) Obtaining the electromagnetic cross-section spectrum of said metalloprotein, (e) Determining from said electromagnetic cross-section spectrum at least one parameter related to one or several analytes of interest.

First claim

The invention claimed is:

1. A method for analyzing an analyte comprising the steps of:
(a) providing a multiscattering medium that enhances a detection of an electromagnetic cross-section signal of a metalloprotein,
(b) incorporating the metalloprotein to be analyzed into the multiscattering medium and the analyte is arranged to establish a redox reaction with the metalloprotein,
(c) contacting the multiscattering medium with electromagnetic radiation,
(d) obtaining the electromagnetic cross-section spectrum of the metalloprotein, and
(e) determining from the electromagnetic cross-section spectrum a concentration of the analyte.

2. The method according to claim 1 wherein the electromagnetic cross-section is an absorption cross-section of the metalloprotein.

3. The method according to claim 1 wherein the multiscattering medium is at least one of an aggregate; a suspension; a gel; a porous membrane; a microstructured substrate; and a nanostructured substrate.

4. The method according to claim 1 wherein the analyte is a reaction product of enzymatic or chemical reactions.

5. The method according to claim 1 wherein the step of (c) contacting uses a dark-field electromagnetic radiation illumination.

6. The method according to claim 1 wherein the step of (e) determining further includes:
determining the concentration of the analyte based on a measurement of a molecular state of the metalloprotein.

7. The method according to claim 6 wherein the molecular state is an oxidation state.

8. The method according to claim 1 wherein the analyte is hydrogen peroxide.

9. The method according to claim 1 wherein the metalloprotein is a cytochrome c.

10. The method according to claim 1 , wherein the multiscattering medium enhances an optical cross-section of the metalloprotein.

11. The method according to claim 1 , wherein the multiscattering medium provides for an amplification of the electromagnetic cross-section spectrum to suppress a background signal.

Assignees

• Ecole Polytechnique Fed De Lausanne (Epfl)
• Ecole Polytechnique Fed Lausanne Epfl

Inventors

• Suárez Guillaume
• Santschi Christian
• Martin Olivier

Classifications

• G01N21/314 Primary

  with comparison of measurements at specific and non-specific wavelengths (dual wavelength spectrometry G01J3/427) · CPC title

• G01N33/573 Primary

  for enzymes or isoenzymes · CPC title

• C12Q1/26

  involving oxidoreductase · CPC title