Methods of stratifying patients for treatment with retinoic acid receptor-alpha agonists

Patent metadata

Field	Value
Publication number	US-9845508-B2
Application number	US-201615172045-A
Country	US
Kind code	B2
Filing date	Jun 2, 2016
Priority date	Mar 31, 2015
Publication date	Dec 19, 2017
Grant date	Dec 19, 2017

Abstract

The invention provides methods that define cellular populations that are sensitive to RARA agonists and identify patient subgroups that will benefit from treatment with RARA agonists. The invention also provides packaged pharmaceutical compositions that comprise a RARA agonist and instructions for determining if such agonist is suitable for use in treatment.

First claim

We claim:

1. A method of diagnosing and treating a human subject suffering from non-APL AML, the method comprising:
a. diagnosing whether the subject has a tamibarotene-sensitive form of the disease based on a level of retinoic acid receptor alpha mRNA previously determined to be present in a sample of diseased cells from the subject, wherein the retinoic acid receptor alpha mRNA is transcribed from a RARA gene that encodes a functional retinoic acid receptor-α gene and specifically excludes gene fusions that comprise all or a portion of the RARA gene; and
b. administering to the subject an amount of tamibarotene effective to treat the disease, wherein the level of retinoic acid receptor alpha mRNA is equal to or above a pre-determined threshold.

2. The method of claim 1 , wherein the pre-determined threshold is a cutoff value.

3. The method of claim 2 , wherein the cutoff value is set at a value that is equal to or up to 5% above the RARA mRNA level in the lowest responder in a population of non-APL AML test samples.

4. The method of claim 3 wherein the test samples are samples obtained from non-APL AML patient-derived xenographs.

5. The method of claim 4 , wherein the cutoff value is the RARA mRNA level in xenograph AM5512.

6. The method of claim 1 , wherein the level of retinoic acid receptor alpha mRNA in the subject is determined using qPCR.

7. The method of claim 2 , wherein the cutoff value is determined using qPCR.

Assignees

• Syros Pharmaceuticals Inc

Inventors

• Chen Mei Wei
• Collins Cindy
• Eaton Matthew Lucas
• Guenther Matthew G
• Ke nan
• Lopez Jeremy
• Mckeown Michael R
• Orlando David A

Classifications

• A61P35/02

  specific for leukemia · CPC title

• A61P35/00

  Antineoplastic agents · CPC title

• A61P15/14

  for lactation disorders, e.g. galactorrhoea · CPC title

• A61K31/165

  having aromatic rings, e.g. colchicine, atenolol, progabide · CPC title

• C12Q2600/156

  Polymorphic or mutational markers · CPC title