Plant regulatory elements and uses thereof

Patent metadata

Field	Value
Publication number	US-9845477-B2
Application number	US-201214117342-A
Country	US
Kind code	B2
Filing date	May 11, 2012
Priority date	May 13, 2011
Publication date	Dec 19, 2017
Grant date	Dec 19, 2017

Abstract

The invention provides DNA molecules and constructs, including their nucleotide sequences, useful for modulating gene expression in plants and plant cells. Transgenic plants, plant cells, plant parts, seeds, and commodity products comprising the DNA molecules operably linked to heterologous transcribable polynucleotides are also provided, as are methods of their use.

First claim

What is claimed is:

1. A DNA molecule exhibiting a gene regulatory functional activity comprising a polynucleotide sequence selected from the group consisting of:
a) a sequence with at least 95 percent sequence identity to any one of SEQ ID NOs: 1, 2, 5, 6, 7, 8, 9, 10, 11, and 12, and exhibiting promoter activity;
b) a sequence comprising any one of SEQ ID NOs: 1, 2, 5, 6, 7, 8, 9, 10, 11, and 12;
and
c) a fragment of any one of SEQ ID NOs: 1, 2, 5, 6, 7, 8, 9, 10, and 11, wherein said fragment comprises SEQ ID NO: 12, and exhibiting promoter activity;
wherein said DNA molecule is operably linked to a heterologous transcribable polynucleotide molecule.

2. The DNA molecule of claim 1 , wherein the heterologous transcribable polynucleotide molecule comprises a gene of agronomic interest.

3. The DNA molecule of claim 2 , wherein the gene of agronomic interest confers herbicide tolerance in plants.

4. The DNA molecule of claim 2 , wherein the gene of agronomic interest confers pest resistance in plants.

5. A transgenic plant cell comprising a DNA molecule exhibiting a gene regulatory functional activity comprising a polynucleotide sequence selected from the group consisting of:
a) a sequence with at least 95 percent sequence identity to any one of SEQ ID NOs: 1, 2, 5, 6, 7, 8, 9, 10, 11, and 12, and exhibiting promoter activity;
b) a sequence comprising any one of SEQ ID NOs: 1, 2, 5, 6, 7, 8, 9, 10, 11, and 12;
and
c) a fragment of any one of SEQ ID NOs: 1, 2, 5, 6, 7, 8, 9, 10, and 11, wherein said fragment comprises SEQ ID NO: 12, and exhibiting promoter activity;
wherein said DNA molecule is operably linked to a heterologous transcribable polynucleotide molecule.

6. The transgenic plant cell of claim 5 , wherein said transgenic plant cell is a monocotyledonous plant cell.

7. The transgenic plant cell of claim 5 , wherein said transgenic plant cell is a dicotyledonous plant cell.

8. A transgenic plant, or part thereof, comprising a DNA molecule exhibiting a gene regulatory functional activity comprising a polynucleotide sequence selected from the group consisting of:
a) a sequence with at least 95 percent sequence identity to any one of SEQ ID NOs: 1, 2, 5, 6, 7, 8, 9, 10, 11, and 12, and exhibiting promoter activity;
b) a sequence comprising any one of SEQ ID NOs: 1, 2, 5, 6, 7, 8, 9, 10, 11, and 12;
and
c) a fragment of any one of SEQ ID NOs: 1, 2, 5, 6, 7, 8, 9, 10, and 11, wherein said fragment comprises SEQ ID NO: 12, and exhibiting promoter activity;
wherein said DNA molecule is operably linked to a heterologous transcribable polynucleotide molecule.

9. A progeny plant of the transgenic plant of claim 8 , or part thereof, wherein the progeny plant or part thereof comprises said DNA molecule exhibiting a gene regulatory functional activity.

10. A transgenic seed comprising a DNA molecule exhibiting a gene regulatory functional activity comprising a polynucleotide sequence selected from the group consisting of:
a) a sequence with at least 95 percent sequence identity to any one of SEQ ID NOs: 1, 2, 5, 6, 7, 8, 9, 10, 11, and 12, and exhibiting promoter activity;
b) a sequence comprising any one of SEQ ID NOs: 1, 2, 5, 6, 7, 8, 9, 10, 11, and 12;
and
c) a fragment of any one of SEQ ID NOs: 1, 2, 5, 6, 7, 8, 9, 10, and 11, wherein said fragment comprises SEQ ID NO: 12, and exhibiting promoter activity;
wherein said DNA molecule is operably linked to a heterologous transcribable polynucleotide molecule.

11. A method of producing a commodity product comprising:
a) obtaining a transgenic plant or part thereof comprising a DNA molecule exhibiting a gene regulatory functional activity comprising a polynucleotide sequence selected from the group consisting of:
1) a sequence with at least 95 percent sequence identity to any one of SEQ ID NOs: 1, 2, 5, 6, 7, 8, 9, 10, 11, and 12, and exhibiting promoter activity;
2) a sequence comprising any one of SEQ ID NOs: 1, 2, 5, 6, 7, 8, 9, 10, 11, and 12;
and
3) a fragment of any one of SEQ ID NOs: 1, 2, 5, 6, 7, 8, 9, 10, and 11, wherein said fragment comprises SEQ ID NO: 12, and exhibiting promoter activity;

wherein said DNA molecule is operably linked to a heterologous transcribable polynucleotide molecule; and
b) producing the commodity product from the transgenic plant or part thereof.

12. The method of claim 11 , wherein the commodity product is protein concentrate, protein isolate, grain, starch, seeds, meal, flour, biomass, or seed oil.

13. A commodity product comprising a DNA molecule exhibiting a gene regulatory functional activity comprising a polynucleotide sequence selected from the group consisting of:
a) a sequence with at least 95 percent sequence identity to any one of SEQ ID NOs: 1, 2, 5, 6, 7, 8, 9, 10, 11, and 12, and exhibiting promoter activity;
b) a sequence comprising any one of SEQ ID NOs: 1, 2, 5, 6, 7, 8, 9, 10, 11, and 12;
and
c) a fragment of any one of SEQ ID NOs: 1, 2, 5, 6, 7, 8, 9, 10, and 11, wherein said fragment comprises SEQ ID NO: 12, and exhibiting promoter activity;
wherein said DNA molecule is operably linked to a heterologous transcribable polynucleotide molecule.

14. A method of expressing a heterologous transcribable polynucleotide molecule comprising:
a) obtaining a transgenic plant comprising a DNA molecule exhibiting a gene regulatory functional activity comprising a polynucleotide sequence selected from the group consisting of:
1) a sequence with at least 95 percent sequence identity to any one of SEQ ID NOs: 1, 2, 5, 6, 7, 8, 9, 10, 11, and 12, and exhibiting promoter activity;
2) a sequence comprising any one of SEQ ID NOs: 1, 2, 5, 6, 7, 8, 9, 10, 11, and 12;
and
3) a fragment of any one of SEQ ID NOs: 1, 2, 5, 6, 7, 8, 9, 10, and 11, wherein said fragment comprises SEQ ID NO: 12, and exhibiting promoter activity;

wherein said DNA molecule 1s operably linked to the heterologous transcribable polynucleotide molecule; and
b) cultivating said transgenic plant, wherein the heterologous transcribable polynucleotide is expressed.

Assignees

• Flasinski Stanislaw
• Foat Barrett C
• Oufattole Mohammed
• Shultz Randall W
• Wei Xiaoping
• Wu Wei
• Yang Shiaw-Pyng
• Monsanto Technology Llc

Inventors

• Flasinski Stanislaw
• Foat Barrett C
• Oufattole Mohammed
• Shultz Randall W
• Wei Xiaoping
• Wu Wei
• Yang Shiaw-Pyng

Classifications

• C07K14/415 Primary

  from plants · CPC title

• C12N15/8216 Primary

  Methods for controlling, regulating or enhancing expression of transgenes in plant cells · CPC title

• C12N15/82 Primary

  for plant cells {, e.g. plant artificial chromosomes (PACs)} · CPC title

• A01H5/00

  Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title