Antibody against human HIF hydroxylase

Patent metadata

Field	Value
Publication number	US-9844546-B2
Application number	US-201514713085-A
Country	US
Kind code	B2
Filing date	May 15, 2015
Priority date	Mar 21, 2001
Publication date	Dec 19, 2017
Grant date	Dec 19, 2017

Abstract

A novel class of hydroxylases is described having the amino acid sequence of SEQ ID NO: 2, 4, 6 and 8, and variants and fragments thereof having HIF hydroxylation activity. The polypeptides of the invention have in particular prolyl hydroxylase activity. An assay method monitors the interaction of the HIF hydroxylase with a substrate. Modulators of HIF hydroxylase are provided for use in the treatment of a condition associated with increased or decreased HIF levels or activity or for the treatment of a condition where it is desirable to modulate HIF levels or activity.

First claim

The invention claimed is:

1. An antibody or a fragment thereof capable of specifically binding a polypeptide comprising:
(a) the amino acid sequence of SEQ ID NO:2 having HIF (hypoxia-inducible factor) hydroxylase activity; or
(b) a variant thereof having at least 80% identity to the amino acid sequence of SEQ ID NO:2 and having HIF hydroxylase activity.

2. The antibody or fragment of claim 1 comprising a variant thereof having at least 90% identity to the amino acid sequence of SEQ ID NO:2 and having hydroxylase activity.

3. The antibody or fragment of claim 2 comprising a variant thereof having at least 95% identity to the amino acid sequence of SEQ ID NO:2 and having hydroxylase activity.

4. An antibody or a fragment thereof capable of specifically binding a polypeptide comprising:
(a) the amino acid sequence of SEQ ID NO:6 having HIF hydroxylase activity; or
(b) a variant thereof having at least 80% identity to the amino acid sequence of SEQ ID NO:6 and having HIF hydroxylase activity.

5. The antibody or fragment of claim 4 comprising a variant thereof having at least 90% identity to the amino acid sequence of SEQ ID NO:6 and having hydroxylase activity.

6. The antibody or fragment of claim 5 comprising a variant thereof having at least 95% identity to the amino acid sequence of SEQ ID NO:6 and having hydroxylase activity.

Assignees

• Univ Oxford Innovation Ltd

Inventors

• Maxwell Patrick Henry
• Pugh Christopher William
• Ratcliffe Peter John
• Schofield Christopher Joseph

Classifications

• A61P35/00

  Antineoplastic agents · CPC title

• A61P9/10

  for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis · CPC title

• A61P43/00

  Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title

• A61P37/06

  Immunosuppressants, e.g. drugs for graft rejection · CPC title

• A61P9/12

  Antihypertensives · CPC title