Imaging flow cytometer using spatial-temporal transformation
US-2024094129-A1 · Mar 21, 2024 · US
US9841306B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9841306-B2 |
| Application number | US-201514930859-A |
| Country | US |
| Kind code | B2 |
| Filing date | Nov 3, 2015 |
| Priority date | Mar 13, 2013 |
| Publication date | Dec 12, 2017 |
| Grant date | Dec 12, 2017 |
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Disclosed is a volume sensor having first, second, and third laser sources emitting first, second, and third laser beams; first, second, and third beam splitters splitting the first, second, and third laser beams into first, second, and third beam pairs; first, second, and third optical assemblies expanding the first, second, and third beam pairs into first, second, and third pairs of parallel beam sheets; fourth, fifth, and sixth optical assemblies focusing the first, second, and third beam sheet pairs into fourth, fifth, and sixth beam pairs; and first, second, and third detector pairs receiving the fourth, fifth, and sixth beam pairs and converting a change in intensity of at least one of the beam pairs resulting from an object passing through at least one of the first, second, and third parallel beam sheets into at least one electrical signal proportional to a three-dimensional representation of the object.
Opening claim text (preview).
We claim: 1. A method of non-contact volume measurement of an object, the method comprising the steps of: emitting a plurality of light beams from three light sources on three axes; acquiring data on a plurality of light intensities received from the plurality of light beams; identifying a change in intensity in at least one of the plurality of received light beams; determining a presence of the object when the change in light intensity exceeds a predetermined magnitude in a predetermined number of received light beams; determining an end of the presence of the object when the change in light intensity falls below the predetermined magnitude in the predetermined number of received light sources; splitting the plurality of light beams into a plurality of beam pairs; expanding the plurality of beam pairs into a plurality of beam sheet pairs; directing the plurality of beam sheet pairs across an object aperture so that the object casts shadows on a collecting lens as the object passes through the plurality of beam sheet pairs; and focusing each of the plurality of beam sheet pairs into focused beam pairs. 2. The method of claim 1 further comprising the step of polarizing each of the plurality of light beams. 3. The method of claim 1 further comprising the step of converting changes in beam intensity of the focused beam pairs into electrical signals proportional to an object cross-section. 4. The method of claim 3 further comprising the step of converting changes in beam intensity of the focused beam pairs into electrical signals proportional to a three-dimensional representation of the object. 5. The method of claim 1 further comprising the step of converting changes in beam intensity of the shadowed beam pairs into electrical signals proportional to a three-dimensional representation of the object. 6. The method of claim 5 further comprising the step of preforming a three-dimensional sizing analysis on the object. 7. The method of claim 6 further comprising the step of categorizing the object according to the three-dimensional sizing analysis.
using a plurality of fixed, simultaneously operating transducers ({G01B11/2408 - G01B11/2425, } G01B11/255 take precedence) · CPC title
Methods or apparatus for determining the capacity of containers or cavities, or the volume of solid bodies (measuring linear dimensions to determine volume G01B) · CPC title
for measuring contours or curvatures · CPC title
specially adapted for measuring length or width of objects while moving · CPC title
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