Method for producing an L-amino acid belonging to the glutamate family, using a coryneform bacterium
US-9029104-B2 · May 12, 2015 · US
Method for producing an L-amino acid using a bacterium of the family Enterobacteriaceae having a disrupted putrescine degradation pathway
US9840725B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9840725-B2 |
| Application number | US-201615097500-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 13, 2016 |
| Priority date | Oct 28, 2013 |
| Publication date | Dec 12, 2017 |
| Grant date | Dec 12, 2017 |
How to read this patent
A practical reading order for non-experts. Skip the full description unless you need deep technical detail.
- Title
What the patent document calls the invention.
- Abstract
A short plain-language summary of the technical disclosure.
- Assignees and inventors
Who owns or filed the patent and who is credited as inventor.
- Key dates
Filing, priority, publication, and grant dates set the timeline.
- First independent claim
The legal scope of protection — read this for what is actually claimed.
- CPC / IPC classifications
Technology tags used to group this patent with similar filings.
- Citations and related patents
Prior art links and similar publications in this corpus.
Abstract
Official abstract text for this publication.
The present invention provides a method for producing L-amino acids such as L-amino acids belonging to the glutamate family by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia , which has been modified to disrupt the putrescine degradation pathway by, for example, inactivation of one gene or several genes from the puuADRCBE gene cluster.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method for producing an L-amino acid comprising: (i) cultivating an L-amino acid-producing bacterium of the family Enterobacteriaceae in a culture medium; and (ii) collecting said L-amino acid in the culture medium, wherein said bacterium has been modified to disrupt a putrescine degradation pathway, wherein said bacterium belongs to the genus Escherichia , and wherein said putrescine degradation pathway is disrupted by a method selected from the group consisting of: (a) attenuation of expression of a gene selected from the group consisting of patA, patD, gabT, gabD, puuA, puuB, puuC, puuD, puuE, and combinations thereof, by mutating said gene, and (b) attenuation of expression of at least one gene from the puuADRCBE gene cluster by mutating said gene, with the proviso that the puuR gene cannot be the only gene that is attenuated; and wherein said L-amino acid is L-arginine or L-omithine. 2. The method according to claim 1 , wherein said putrescine degradation pathway is a transaminase pathway or a glutamylated putrescine pathway. 3. The method according to claim 1 , wherein said putrescine degradation pathway is disrupted by mutating the puuA gene or the entire puuADRCBE gene cluster. 4. The method according to claim 1 , wherein said gene is inactivated. 5. The method according to claim 4 , wherein said gene is deleted. 6. The method according to claim 1 , wherein said bacterium is Escherichia coli.
Assignees
Inventors
Classifications
Patent family
Related publications grouped by family.
External sources
Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US9840725B2 cover?
- The present invention provides a method for producing L-amino acids such as L-amino acids belonging to the glutamate family by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia , which has been modified to disrupt the putrescine degradation pathway by, for example, inactivation of one gene or several genes from the puuA…
- Who is the assignee on this patent?
- Ajinomoto Kk
- What technology area does this patent fall under?
- Primary CPC classification C12P13/10. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Dec 12 2017 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 1 related publication on this page (citations in our corpus or others sharing the same primary CPC).