Polypeptide hydrogels and uses related thereto

Patent metadata

Field	Value
Publication number	US-9840693-B2
Application number	US-201715411125-A
Country	US
Kind code	B2
Filing date	Jan 20, 2017
Priority date	Jan 23, 2014
Publication date	Dec 12, 2017
Grant date	Dec 12, 2017

Abstract

This disclosure relates to self-assembling peptides that form hydrogels comprising peptide sequences for the activation of signaling pathways. In certain embodiments, the disclosure relates to compositions comprising a recombinant polypeptide comprising a cell signaling sequence, e.g., JAG-1 sequence, fused to a hydrogel polypeptide sequence. In certain embodiments, the disclosure relates to methods of cell culture on three dimensional scaffolds/hydrogels composed of self-assembling peptides disclosed herein.

First claim

The invention claimed is:

1. A method of growing cells comprising placing an isolated cell on or in a hydrogel under conditions such that the cell replicates,
wherein hydrogel comprises a recombinant polypeptide in combination with a hydrogel polypeptide,
wherein the recombinant polypeptide comprises a cell signaling sequence, wherein the cells signal sequence comprises SEQ ID NO: 1, fused to a hydrogel polypeptide sequence,
wherein the hydrogel polypeptide and the recombinant polypeptide are in a ratio of between 1:5 to 1:15 by weight, and
wherein the hydrogel polypeptide and the recombinant peptide are 1.5% to 2.5% by weight to volume of the hydrogel.

2. The method of claim 1 , wherein the isolated cell is a stem cell, progenitor cell, cardiac stem cell, cardiac progenitor cell, bone marrow derived cell, or mesenchymal stem cell.

3. The method of claim 2 , wherein the cardiac progenitor cell is isolated by the process of selecting cells for stem cell surface markers, progenitor cell surface markers, or cardiac cell surface markers.

4. The method of claim 1 , wherein the hydrogel comprises a saccharide at a concentration of between 100 nm to 500 mM.

5. The method of claim 1 , wherein the hydrogel comprises a buffer creating a pH of between 7.0 to 7.7.

6. The method of claim 1 , wherein the hydrogel further comprising a cardiac progenitor cell.

Assignees

• Univ Emory
• Georgia Tech Res Inst

Inventors

• Davis Michael E
• Boopathy Archana V

Classifications

• C07K14/70596

  Molecules with a "CD"-designation not provided for elsewhere · CPC title

• C07K14/00

  Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof · CPC title

• A61K35/34

  Muscles; Smooth muscle cells; Heart; Cardiac stem cells; Myoblasts; Myocytes; Cardiomyocytes (vascular smooth muscle A61K35/44) · CPC title

• C12N2533/50

  Proteins · CPC title

• C12N5/0068

  General culture methods using substrates (for specific animal cell type C12N5/06) · CPC title