Devices and methods for manipulating components in a fluid sample

Patent metadata

Field	Value
Publication number	US-9835540-B2
Application number	US-201615154810-A
Country	US
Kind code	B2
Filing date	May 13, 2016
Priority date	Oct 26, 2012
Publication date	Dec 5, 2017
Grant date	Dec 5, 2017

Abstract

Devices for sorting components (e.g., cells) contained in a liquid sample are provided. In certain aspects, the devices include a magnetic separation device and an acoustic concentrator device fluidically coupled to magnetic separation device. Aspects of the invention further include methods for sorting cells in a liquid sample, and systems, and kits for practicing the subject methods.

First claim

What is claimed is:

1. A method of sorting a liquid sample comprising cells or particles, the method comprising:
a) seperating magnetically labeled cells or particles from non-magnetically labeled cells or particles in the sample, by introducing the sample into a sorting device comprising a magnet that separates the magnetically labeled cells or particles from the non-magnetically labeled cells or particles, thereby creating a first sorted sample; and
b) acoustically concentrating the first sorted sample to produce a second sorted sample by exposing the first sorted sample to acoustic waves generated by an acoustic concentrator, wherein the magnet and the acoustic concentrator are spatially seperated in the device.

2. The method according to claim 1 , comprising collecting the second sorted sample.

3. The method according to claim 1 , comprising analyzing the second sorted sample.

4. The method according to claim 3 , wherein analyzing the second sorted sample comprises flow cytometric analysis.

5. The method according to claim 1 , wherein the second sorted sample has an entrainment factor of about 0.0 to 1.0.

6. The method according to claim 1 , further comprising labeling the cells or particles in the liquid sample with a magnetic label before the separating step.

7. The method according to claim 1 , wherein the method is controlled by a processor.

8. The method according to claim 1 , wherein the processor controls the method under a closed-loop feedback mechanism.

9. The method according to claim 1 , wherein the liquid sample comprises a biological sample.

10. The method according to claim 1 , wherein the liquid sample is obtained from a human.

Assignees

• Becton Dickinson Co

Inventors

• Yu Liping
• Warner Brian David
• Trotter Joseph T

Classifications

• G01N33/54326 Primary

  Magnetic particles · CPC title

• G01N1/4077

  by other techniques involving separation of suspended solids · CPC title

• B03C2201/26

  for use in medical or biological applications · CPC title

• B01L2300/0877

  Flow chambers · CPC title

• B01L2400/043

  magnetic forces · CPC title