Tissue processing reagent

Patent metadata

Field	Value
Publication number	US-9835527-B2
Application number	US-201514984946-A
Country	US
Kind code	B2
Filing date	Dec 30, 2015
Priority date	Dec 30, 2015
Publication date	Dec 5, 2017
Grant date	Dec 5, 2017

Abstract

A method including treating a biological sample taken from a body with a composition including an acetal solvent. A method including contacting a fixed biological sample taken from a body with a composition including an acetal solvent as at least one a dehydrating, clearing and infiltration process.

First claim

What is claimed is:

1. A method comprising:
treating a biological sample taken from a body with a composition comprising an acetal solvent; and
after treating the biological sample, containing the biological sample with a paraffin.

2. The method of claim 1 , wherein the acetal solvent comprises a dioxolane.

3. The method of claim 2 , wherein the dioxolane comprises 1,3-dioxolane.

4. The method of claim 1 , wherein prior to treating the biological sample with the composition, the method comprises fixing the biological sample.

5. The method of claim 4 , wherein after fixing the biological sample, the method comprises dehydrating, clearing and infiltrating the biological sample, and treating the biological sample with the composition comprises treating as at least a part of infiltrating the biological tissue.

6. The method of claim 5 , wherein the composition further comprises a paraffin.

7. The method of claim 4 , wherein after fixing the biological sample, the method comprises dehydrating and clearing the biological sample and treating the biological sample with the composition comprises treating as at least a part of at least one of dehydrating and clearing.

8. The method of claim 7 , wherein dehydrating comprises treating the fixed biological sample with a dehydrating composition comprising the composition.

9. The method of claim 7 , wherein prior to treating the biological sample with the composition, the method comprises dehydrating the fixed biological sample.

10. The method of claim 9 , wherein dehydrating comprises treating the fixed biological sample with a dehydrating composition comprising an alcohol.

11. The method of claim 9 , wherein the dehydrating composition comprises at least one of acetone and a glycol.

12. A method comprising:
treating a fixed biological sample taken from a body to a dehydrating process, a clearing process and an infiltration process, wherein at least one of the dehydrating process, the clearing process and the infiltration process comprises contacting the fixed biological sample with a composition comprising an acetal solvent and wherein the infiltration process comprises infiltrating the fixed biological sample with an embedding agent.

13. The method of claim 12 , wherein the acetal solvent comprises a dioxolane.

14. The method of claim 13 , wherein the dioxolane comprises 1,3-dioxolane.

15. The method of claim 12 , wherein the clearing process comprises contacting the fixed biological sample with a composition comprising an acetal solvent.

16. The method of claim 15 , wherein the clearing process further comprises contacting the biological sample with another clearing agent.

17. The method of claim 16 , wherein the another clearing agent comprises at least one of a hydrocarbon clearing agent and a hydrocarbon clearing agent with an oxygen-based functional group.

18. The method of claim 16 , wherein the acetal solvent comprises a dioxolane and the acetal solvent and the another clearing agent comprise a mixture.

19. The method of claim 12 , wherein the dehydrating process comprises contacting the fixed biological sample with a composition comprising an acetal solvent.

20. The method of claim 12 , wherein the dehydrating process further comprises contacting the biological sample with an alcohol.

21. The method of claim 20 , wherein the acetal solvent and the alcohol comprise a mixture.

22. The method of claim 12 , wherein the infiltration process comprises contacting the fixed biological sample with a composition comprising an acetal solvent.

23. The method of claim 22 , wherein the embedding agent comprises a paraffin.

24. The method of claim 22 , wherein the dioxolane and the embedding agent comprise a mixture.

Assignees

• Sakura Finetek Usa Inc

Inventors

• Shah Amit D

Classifications

• G01N1/30 Primary

  Staining; Impregnating {; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis} · CPC title

• G01N2001/305

  Fixative compositions · CPC title