Biofuel and chemical production by recombinant microorganisms via fermentation of proteinaceous biomass
US-9284566-B2 · Mar 15, 2016 · US
US9834796B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9834796-B2 |
| Application number | US-201214343404-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 6, 2012 |
| Priority date | Sep 7, 2011 |
| Publication date | Dec 5, 2017 |
| Grant date | Dec 5, 2017 |
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The present invention relates to a method for producing useful metabolites such as shikimic acid from filamentous fungus. The useful metabolites can be produced by a production method involving a step of inhibiting the growth of the filamentous fungus, specifically by applying a stimulus of light having a center wavelength shorter than 570 nm to the filamentous fungus, to increase the content of the useful metabolite in a hypha of the filamentous fungus.
Opening claim text (preview).
The invention claimed is: 1. A method for producing useful metabolites comprising a process of suppressing the growth of filamentous fungi by applying light stimulation to filamentous fungi, and a process of extracting useful metabolites from filamentous fungi; wherein the useful metabolite is shikimic acid, and wherein the light stimulation is a stimulus of light having a center wavelength shorter than 570 nm, thereby the biosynthetic pathway of aromatic amino acids is blocked after shikimic acid is biosynthesized. 2. The method according to claim 1 , wherein the light stimulation is a stimulus of blue light and/or a stimulus of light having a photon flux density not less than 11 μmol m −2 s −1 . 3. The method according to claim 1 , wherein the light stimulation is applied with intermittent irradiation. 4. The method according to claim 1 , wherein the extraction is carried out from filamentous fungi in mycelial state. 5. The method according to claim 1 , wherein the filamentous fungus is Pleurotus ostreatus. 6. The method according to claim 1 , wherein the extraction comprises separation into a water layer and an organic layer using an organic solvent and water, and collection of the water layer.
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