Recombinant microorganisms and uses therefor

Patent metadata

Field	Value
Publication number	US-9834795-B2
Application number	US-201615205891-A
Country	US
Kind code	B2
Filing date	Jul 8, 2016
Priority date	Feb 25, 2011
Publication date	Dec 5, 2017
Grant date	Dec 5, 2017

Abstract

The invention provides recombinant microorganisms and methods for the production of acetone from gaseous substrates. For example, the recombinant microorganism may be modified to express an exogenous thiolase, an exogenous CoA transferase, and an exogenous decarboxylase.

First claim

The invention claimed is:

1. A method of producing acetone comprising culturing a recombinant microorganism in the presence of a gaseous substrate comprising one or more of CO, CO 2 , and H 2 to produce acetone, wherein the recombinant microorganism comprises an exogenous thiolase (EC 2.3.1.9), an exogenous CoA transferase (EC 2.8.3.9), and an exogenous decarboxylase (EC 4.1.1.4 or EC 4.1.1.74), wherein the recombinant microorganism is derived from a parental microorganism selected from the group consisting of Clostridium autoethanogenum, Clostridium ljungdahlii , and Clostridium ragsdalei.

2. The method of claim 1 , wherein the recombinant microorganism is a carboxydotrophic and acetogenic bacterium.

3. The method of claim 1 , wherein the recombinant microorganism is derived from a parental microorganism that does not produce acetone.

4. The method of claim 1 , wherein the parental microorganism is a microorganism that occurs in nature or a microorganism that has been previously modified.

5. The method of claim 1 , wherein the recombinant microorganism is derived from a parental microorganism that lacks a gene encoding alcohol dehydrogenase.

6. The method of claim 1 , wherein the thiolase is Clostridium acetobutylicum ThlA or Clostridium beijerinckii ThlA.

7. The method of claim 1 , wherein the CoA transferase is Clostridium acetobutylicum CtfA and CtfB or Clostridium beijerinckii CtfA and CtfB.

8. The method of claim 1 , wherein the decarboxylase is acetoacetate decarboxylase (EC 4.1.1.4).

9. The method of claim 8 , wherein the acetoacetate decarboxylase is Clostridium acetobutylicum Adc or Clostridium beijerinckii Adc.

10. The method of claim 1 , wherein the decarboxylase is alpha-ketoisovalerate decarboxylase (EC 4.1.1.74).

11. The method of claim 10 , wherein the alpha-ketoisovalerate decarboxylase is Lactococcus lactis KivD.

12. The method of claim 1 , wherein the gaseous substrate comprises 20-70% CO.

13. The method of claim 1 , wherein the gaseous substrate is derived from an industrial process selected from the group consisting of ferrous metal products manufacturing, non-ferrous products manufacturing, petroleum refining, coal gasification, biomass gasification, electric power production, carbon black production, and coke manufacturing.

14. The method of claim 1 , wherein the gaseous substrate is syngas.

Assignees

• Lanzatech New Zealand Ltd

Inventors

• Koepke Michael
• Simpson Sean
• Liew Fungmin
• Chen Wendy Yiting

Classifications

• C12N15/52 Primary

  Genes encoding for enzymes or proenzymes · CPC title

• C12N15/74

  Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora · CPC title

• C12N9/13

  transferring sulfur containing groups (2.8) · CPC title

• C12N9/0006

  acting on CH-OH groups as donors (1.1) · CPC title

• C12P7/30 Primary

  produced from substrate containing inorganic compounds other than water · CPC title