Methods, systems, and computer program products for detecting pipette tip integrity

Patent metadata

Field	Value
Publication number	US-9833779-B2
Application number	US-201514826749-A
Country	US
Kind code	B2
Filing date	Aug 14, 2015
Priority date	Aug 15, 2014
Publication date	Dec 5, 2017
Grant date	Dec 5, 2017

Abstract

This invention relates to methods, systems, and computer program products for detecting pipette tip integrity.

First claim

What is claimed is:

1. A method comprising:
collecting pressure data while aspirating a gas into a pipette tip attached to a pipette, the pressure data including a plurality of pressure values measured at an internal portion of the pipette and taken over a given time interval, the plurality of pressure values including a maximum pressure value and a minimum pressure value;
estimating a pressure range value between the maximum pressure value and the minimum pressure value;
responsive to the pressure range value being greater than or equal to a lower threshold and the pressure range value being less than or equal to an upper threshold, determining that the pipette tip attached to the pipette is properly functioning, or
responsive to the pressure range value being less than a lower threshold or the pressure range value being greater than an upper threshold, determining that the pipette tip attached to the pipette is not properly functioning; and
responsive to the pressure range value being greater than or equal to the lower threshold and the pressure range value being less than or equal to the upper threshold, determining that the pipette tip attached to the pipette is suitable for use, or
responsive to the pressure range value being less than the lower threshold or the pressure range value being greater than the upper threshold, determining that the pipette tip is defective, clogged, and/or improperly attached to the pipette.

2. The method of claim 1 , wherein collecting pressure data while aspirating the gas into the pipette tip attached to the pipette comprises measuring pipette pressure at given time intervals.

3. The method of claim 2 , wherein aspirating the gas into the pipette tip attached to the pipette further comprises aspirating the gas into the pipette tip attached to the pipette over a given time interval.

4. The method of claim 1 , wherein collecting pressure data while aspirating the gas into the pipette tip attached to the pipette comprises aspirating the gas at a given rate of aspiration.

5. The method of claim 4 , wherein the given rate of aspiration is in a range of about 300 μL/sec to about 700 μL/sec.

6. The method of claim 1 , wherein collecting pressure data while aspirating the gas into the pipette tip attached to the pipette comprises aspirating the gas prior to aspirating a liquid into the pipette tip.

7. The method of claim 1 , wherein estimating the pressure range value between the maximum pressure value and the minimum pressure value comprises subtracting the minimum pressure value from the maximum pressure value to obtain the pressure range value.

8. The method of claim 1 , wherein at least one of the upper threshold and the lower threshold is determined using a given rate of aspiration.

9. The method of claim 8 , wherein the given rate of aspiration is in a range of about 300 μL/sec to about 700 μL/sec.

10. The method of claim 8 , wherein the given rate of aspiration is the same as the rate of aspiration for aspirating the gas into the pipette tip attached to the pipette.

11. The method of claim 1 , further comprising, responsive to the pressure range value being greater than or equal to the lower threshold and the pressure range value being less than or equal to the upper threshold, aspirating a liquid into the pipette tip.

12. The method of claim 1 , further comprising, responsive to the pressure range value being less than the lower threshold or the pressure range value being greater than the upper threshold, removing the pipette tip from the pipette.

13. The method of claim 1 , further comprising detecting that the pipette tip is defective or without a filter responsive to the pressure range value being less than the lower threshold.

14. The method of claim 1 , further comprising detecting that the pipette tip is defective responsive to the pressure range value being greater than the upper threshold.

15. The method of claim 1 , wherein the maximum pressure value is measured at a first point in time and the minimum pressure value is measured at a second point in time and the first point in time is before the second point in time.

16. The method of claim 1 , wherein the maximum pressure value is measured at a first point in time and the minimum pressure value is measured at a second point in time and the second point in time is before the first point in time.

17. A computer system, comprising:
a processor; and
a memory coupled to the processor, the memory comprising computer readable program code embodied therein that, when executed by the processor, causes the processor to perform operations comprising the method of claim 1 .

18. The computer system of claim 17 , further comprising an apparatus for positioning a pipette and pipetting a liquid onto a surface of a target.

19. The computer system of claim 18 , wherein the apparatus for positioning a pipette and pipetting a liquid onto a surface includes a pipette including a pipette pressure detector.

20. A computer program product comprising:
a non-transitory computer readable storage medium having computer readable code embodied in the medium, the computer code comprising:
computer readable code configured to perform operations comprising the method of claim 1 .

Assignees

• Bio Merieux Inc

Inventors

• Pionke Jeremey Joseph
• Harrison Joel Patrick
• Luebbert Daniel Oliver
• Pingel Daniel Joseph

Classifications

• B01L2200/146

  Employing pressure sensors · CPC title

• G01N2035/103

  using disposable tips · CPC title

• B01L2300/0627

  Sensor or part of a sensor is integrated · CPC title

• G01N2035/1018

  Detecting inhomogeneities, e.g. foam, bubbles, clots · CPC title

• G01N35/10

  Devices for transferring samples {or any liquids} to, in, or from, the analysis apparatus, e.g. suction devices, injection devices {(G01N35/0099 takes precedence)} · CPC title