Enhanced anabolic cytokine production and delivery system

Patent metadata

Field	Value
Publication number	US-9833475-B2
Application number	US-201514970574-A
Country	US
Kind code	B2
Filing date	Dec 16, 2015
Priority date	Mar 1, 2013
Publication date	Dec 5, 2017
Grant date	Dec 5, 2017

Abstract

Techniques and devices for removing (filtering out) unwanted/inhibitory components (for example, products, byproducts and/or cell output such as inhibitory catabolic proteins) from a composition (for example, autologous fluid or serum) containing such unwanted/inhibitory components. The devices include at least one construct designed to contain a composition (for example, autologous fluid or serum) containing inhibitory/unwanted components (products, byproducts and/or output of cells such as inhibitory catabolic proteins). The construct is provided with interior walls including specific protein capturing means designed to remove the inhibitory/unwanted component(s) (for example, specific protein(s)) from the composition. Proteins targeted for capture include, but are not limited to, catabolic cytokines such as IL-1β, IL-α, IL-6, TNFα, IFNγ and MMPS as these proteins inhibit the healing process.

First claim

The invention claimed is:

1. A method for selectively removing unwanted components from a composition, comprising:
introducing a composition into a construct having an internal wall construct formation coated with a capture antibody;
passing the composition through the construct or incubating the composition within the construct to bind a catabolic cytokine and render a treated composition; and
removing the treated composition from the construct.

2. The method as recited in claim 1 , comprising storing the treated composition in a refrigerator or freezer for later use to treat damaged tissue of a mammal.

3. The method as recited in claim 1 , wherein incubating the composition includes allowing the composition to sit in the construct for a set time period at room temperature to bind the catabolic cytokine.

4. The method as recited in claim 1 , wherein passing the composition through the construct includes passing the composition back and forth across the internal wall construct formation to bind the catabolic cytokine.

5. The method as recited in claim 1 , wherein the composition is a serum or an autologous fluid.

6. A catabolic cytokines removal system, comprising:
a first syringe configured to hold a non-treated composition;
a second syringe configured to hold a treated composition; and
a tubing construct connected between the first syringe and the second syringe and including an internal wall coated with a capture antibody, the capture antibody configured to capture at least IL-1β as the non-treated composition is passed through the tubing construct to prepare the treated composition.

7. The system as recited in claim 6 , wherein the non-treated composition includes a first IL-1Ra/IL-1β ratio and the treated composition includes a second IL-1Ra/IL-1β ratio that is greater than the first IL-1Ra/IL-1β ratio.

8. The system as recited in claim 6 , comprising a formation on the internal wall that enlarges a surface area of the internal wall.

9. The system as recited in claim 8 , wherein the formation includes internal ribbing formed on the internal wall.

10. The system as recited in claim 6 , wherein the first syringe includes a second internal wall coated with a second capture antibody.

11. The method as recited in claim 1 , wherein the capture antibody comprises an anti-IL-1β antibody.

Assignees

• Arthrex Inc

Inventors

• Bare Christopher M
• Roller Brandon L
• Tucker Melissa S

Classifications

• A61P43/00

  Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title

• C07K16/245

  IL-1 · CPC title

• A61K38/1793

  for cytokines; for lymphokines; for interferons · CPC title

• A61K35/16 Primary

  Blood plasma; Blood serum (umbilical cord blood A61K35/51) · CPC title

• A61J1/2089

  Containers or vials which are to be joined to each other in order to mix their contents (combinations of vial and syringe for mixing their contents A61J1/2096) · CPC title