Functionalized lipid modification of solid phase surfaces for use in chromatography

Patent metadata

Field	Value
Publication number	US-9827552-B2
Application number	US-201414333561-A
Country	US
Kind code	B2
Filing date	Jul 17, 2014
Priority date	Jul 17, 2013
Publication date	Nov 28, 2017
Grant date	Nov 28, 2017

Abstract

A solid phase for use in separation has been modified using an aqueous phase adsorption of a headgroup-modified lipid to generate analyte specific surfaces for use as a stationary phase in separations such as high performance liquid chromatography (HPLC) or solid phase extraction (SPE). The aliphatic moiety of the lipid adsorbs strongly to a hydrophobic solid surface, with the hydrophilic and active headgroups orienting themselves toward the more polar mobile phase, thus allowing for interactions with the desired solutes. The surface modification approach is generally applicable to a diversity of selective immobilization applications such as protein immobilization clinical diagnostics and preparative scale HPLC as demonstrated on capillary-channeled fibers, though the general methodology could be implemented on any hydrophobic solid support material.

First claim

What is claimed is:

1. A surface modified solid phase for a chromatography separation protocol comprising:
a polymeric solid phase comprising a hydrophobic surface;
a lipid, the lipid including a hydrophobic end and a headgroup opposite the hydrophobic end, the hydrophobic end having the structure of:

wherein m is from 6 to 30, the hydrophobic end being adsorbed to the hydrophobic surface of the polymeric solid phase such that the hydrophobic end of the lipid lays along or intercollates into the hydrophobic surface, the headgroup comprising a functional group that is configured for binding a targeted analyte, the functional group including a coenzyme, a nucleotide, or a polypeptide.

2. The surface modified solid phase of claim 1 , wherein the lipid is a phospholipid, a glycerolipid, a glycerophospholipid, or a fatty acid.

3. The surface modified solid phase of claim 1 , further comprising a hydrophilic spacer between the hydrophobic end and the headgroup.

4. The surface modified solid phase of claim 3 , wherein the hydrophilic spacer comprises a polyethylene glycol spacer.

5. The surface modified solid phase of claim 1 , wherein the polymeric solid phase comprises a fiber.

6. The surface modified solid phase of claim 5 , wherein the fiber is a capillary-channeled fiber.

7. The surface modified solid phase of claim 1 , wherein the polymeric solid phase comprises a polyolefin, a polyester, a polyaniline, a polylactic acid, a polyamide, a poly(styrene-divinyl benzene), a methacrylate, a polymer blend and/or a copolymer thereof.

8. A chromatography separation device comprising the surface modified solid phase of claim 1 held in a casing.

9. The chromatography separation device of claim 8 , wherein the casing comprises an interior that is surface modified with the lipid adsorbed thereto.

Assignees

• Univ Clemson

Inventors

• Marcus R Kenneth
• Christensen Kenneth A

Classifications

• B01J20/3293

  Coatings on a core, the core being particle or fiber shaped, e.g. encapsulated particles, coated fibers · CPC title

• B01J20/3274

  Proteins, nucleic acids, polysaccharides, antibodies or antigens · CPC title

• B01J20/28023

  Fibres or filaments (fibres or filaments in the form of membranes B01J20/28038; B01J20/28007 takes precedence) · CPC title

• B01J20/28052

  Several layers of identical or different sorbents stacked in a housing, e.g. in a column · CPC title

• G01N30/50 Primary

  Conditioning of the sorbent material or stationary liquid · CPC title