Magnetic biomedical sensors and sensing system for high-throughput biomolecule testing

Patent metadata

Field	Value
Publication number	US-9823316-B2
Application number	US-201214352638-A
Country	US
Kind code	B2
Filing date	Oct 19, 2012
Priority date	Oct 19, 2011
Publication date	Nov 21, 2017
Grant date	Nov 21, 2017

Abstract

A magnetic biosensor can include a magnetic stack comprising a free layer, a fixed layer, and a nonmagnetic layer between the free layer and the fixed layer. At least one of the free layer or the fixed layer may have a magnetic moment oriented out of a major plane of the free layer or the fixed layer, respectively, in an absence of an external magnetic field. The magnetic biosensor also may include a sample container disposed over the magnetic stack, a plurality of capture antibodies attached to a bottom surface of the sample container above the magnetic stack, and a magnetic field generator configured to generate a magnetic field substantially perpendicular to the major plane of the free layer or fixed layer.

First claim

What is claimed is:

1. A magnetic biosensing system comprising:
a probe array comprising a plurality of probes;
a magnetic biosensor array comprising:
a plurality of electrical contacts located along at least one peripheral edge of the magnetic biosensor array, wherein the plurality of electrical contacts of the magnetic biosensor array are configured to receive respective ones of the plurality of probes;
a sample container;
a plurality of magnetic biosensors each located adjacent to a surface of the sample container, each magnetic biosensor of the plurality of magnetic biosensors comprising a magnetic sensor comprising a free layer and a fixed layer, wherein one of the free layer or the fixed layer has a magnetic moment canted out of a major plane of the free layer or the fixed layer at an inclined angle between 1 and 90 degrees, respectively, in an absence of an external magnetic field, and wherein the other of the free layer or the fixed layer has a magnetic moment oriented parallel to the major plane of the free layer or the fixed layer in the absence of the external magnetic field; and
a plurality of capture antibodies attached to the surface of the sample container;

a motor configured to move the probe array toward and away from the magnetic biosensor array to engage and disengage the plurality of probes and the plurality of electrical contacts; and
a magnetic field generator located below the magnetic biosensor array to apply a magnetic field in a direction perpendicular to a major plane of the magnetic biosensor array.

2. The magnetic biosensing system of claim 1 , wherein the magnetic moment of the fixed layer of at least one magnetic biosensor of the plurality of magnetic biosensors in the absence of the external magnetic field is canted out of the major plane of the fixed layer and the magnetic moment of the free layer in the absence of the external magnetic field is oriented parallel to the major plane of the free layer.

3. The magnetic biosensing system of claim 1 , wherein the magnetic moment of the free layer of at least one magnetic biosensor of the plurality of magnetic biosensors in the absence of the external magnetic field is canted out of the major plane of the free layer and the magnetic moment of the fixed layer in the absence of the external magnetic field is oriented parallel to the major plane of the fixed layer.

4. The magnetic biosensing system of claim 1 , further comprising a current source and a voltage source, wherein each magnetic biosensor of the plurality of magnetic biosensors further comprises:
a nonmagnetic layer comprising a first end and a second end, wherein the free layer is formed adjacent to the first end and the fixed layer is formed adjacent to the second end,
a current source coupling electrically coupled to the free layer, the current source, and the first end of the nonmagnetic layer; and
a voltage source coupling electrically coupled to the fixed layer, the voltage source, and the second end of the nonmagnetic layer, wherein the voltage source is configured to measure a magnetoresistance of the fixed layer and the second end of the nonmagnetic layer.

5. The magnetic biosensing system of claim 1 , wherein a respective one or more of the plurality of electrical contacts is electrically coupled to a respective one or more of the plurality of magnetic biosensors, and wherein respective ones of the plurality of electrical contacts are configured to contact respective ones of a plurality of probes to communicate sensed test data to a computing device.

6. The magnetic biosensing system of claim 1 , wherein the sample container comprises at least one of a sample well or a microfluidic channel.

7. The magnetic biosensing system of claim 1 , wherein the plurality of capture antibodies comprises a first plurality of capture antibodies and a second plurality of capture antibodies, wherein the first plurality of capture antibodies are attached to the bottom surface of the sample container above a first magnetic biosensor of the plurality of magnetic biosensors, and wherein the second plurality of capture antibodies are attached to the bottom surface of the sample container above a second magnetic biosensor of the plurality of magnetic biosensors.

8. The magnetic biosensing system of claim 1 , wherein the magnetic moment of the fixed layer of each magnetic biosensor of the plurality of magnetic biosensors in the absence of the external magnetic field is canted out of the major plane of the fixed layer and the magnetic moment of the free layer in the absence of the external magnetic field is oriented parallel to the major plane of the free layer.

9. The magnetic array biosensing system of claim 1 , wherein the magnetic moment of the free layer of each magnetic biosensor of the plurality of magnetic biosensors in the absence of the external magnetic field is canted out of the major plane of the free layer and the magnetic moment of the fixed layer in the absence of the external magnetic field is oriented parallel to the major plane of the fixed layer.

10. The magnetic biosensing system of claim 1 , further comprising a current source and a voltage source, wherein a magnetic sensor of at least one magnetic biosensor of the plurality of magnetic biosensors further comprises:
a nonmagnetic layer comprising a first end and a second end, wherein the free layer is formed adjacent to the first end and the fixed layer is formed adjacent to the second end,
a current source coupling electrically coupled to the free layer, the current source, and the first end of the nonmagnetic layer; and
a voltage source coupling electrically coupled to the fixed layer, the voltage source, and the second end of the nonmagnetic layer, wherein the voltage source is configured to measure a magnetoresistance of the fixed layer and the second end of the nonmagnetic layer.

11. The magnetic biosensor array of claim 10 , wherein the free magnetic layer comprises a plurality of free magnetic layers, wherein the nonmagnetic layer comprises a plurality of first ends, and wherein respective ones of the plurality of free magnetic layers are formed adjacent to respective ones of the plurality of first ends, and wherein each of the free magnetic layers are electrically coupled to the current source.

12. The magnetic biosensing system of claim 1 , wherein the probe array further comprises a plurality of sample dispensers configured to dispense solutions into the sample container.

13. A method for forming a magnetic biosensing system, the method comprising:
forming a probe array comprising a plurality of probes;
forming a magnetic biosensor array comprising a plurality of electrical contacts located along at least one peripheral edge of the magnetic biosensor array, wherein the plurality of electrical contacts of the magnetic biosensor array are configured to receive respective ones of the plurality of probes;
placing a sample container over the magnetic biosensor array, wherein the magnetic biosensor array comprises a plurality of magnetic biosensors each located adjacent to a surface of the sample container, each magnetic biosensor of the plurality of magnetic biosensors comprising a magnetic sensor comprising a free layer and a fixed layer, wherein one of the free layer or the fixed layer has a magnetic moment canted out of a major plane of the free layer or the fixed layer at an inclined angle between 1 and 90 degrees, respectively, in an absence of an external magnetic field, and wherein the other of the free layer or the fixed layer has a magnetic moment oriented parallel to the major plane of the free layer or the fixed layer in the absence of the extern

Assignees

• Univ Minnesota

Inventors

• Wang Jian-Ping
• Rahman Md Tofizur
• Wang Yi

Classifications

• G01R3/00

  Apparatus or processes specially adapted for the manufacture {or maintenance} of measuring instruments {, e.g. of probe tips} · CPC title

• G01N35/0098

  involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation (magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C) · CPC title

• G01N27/72

  by investigating magnetic variables · CPC title

• G01R33/12 Primary

  Measuring magnetic properties of articles or specimens of solids or fluids (involving magnetic resonance G01R33/20) · CPC title

• B82Y25/00 Primary

  Nanomagnetism, e.g. magnetoimpedance, anisotropic magnetoresistance, giant magnetoresistance or tunneling magnetoresistance · CPC title