HPLC sample introduction with coupling sample reservoirs in parallel between mobile phase drive and separation unit

Patent metadata

Field	Value
Publication number	US-9823226-B2
Application number	US-201314898392-A
Country	US
Kind code	B2
Filing date	Jun 14, 2013
Priority date	Jun 14, 2013
Publication date	Nov 21, 2017
Grant date	Nov 21, 2017

Abstract

Disclosed is a sample dispatcher configured for individually introducing a plurality of portions of one or more sample fluids into a flow of a mobile phase of a separation system configured for separating compounds of the sample fluids. The separation system comprises a mobile phase drive configured for driving the mobile phase through a separation unit configured for separating compounds of the sample fluids in the mobile phase. The sample dispatcher comprises a plurality of sample reservoirs, each configured for receiving and temporarily storing a respective sample fluid portion or at least a part thereof. The sample dispatcher is configured for selectively coupling one of the plurality of sample reservoirs between the mobile phase drive and the separation unit, and further for coupling at least two of the plurality of sample reservoirs in parallel between the mobile phase drive and the separation unit.

First claim

The invention claimed is:

1. A sample dispatcher configured for individually introducing a plurality of portions of one or more sample fluids into a flow of a mobile phase of a separation system configured for separating compounds of the sample fluids, wherein the separation system comprises a mobile phase drive configured for driving the mobile phase through a separation unit configured for separating compounds of the sample fluids in the mobile phase, the sample dispatcher comprising:
a plurality of sample reservoirs, each configured for receiving and temporarily storing a respective sample fluid portion or at least a part thereof;
wherein the sample dispatcher is configured for selectively coupling one of the plurality of sample reservoirs between the mobile phase drive and the separation unit; and
the sample dispatcher is further configured for coupling at least two of the plurality of sample reservoirs in parallel between the mobile phase drive and the separation unit.

2. The sample dispatcher of claim 1 , wherein:
the sample dispatcher is configured for coupling at least two of the plurality of sample reservoirs in parallel between the mobile phase drive and the separation unit during a dilution state of changing from having one of the plurality of sample reservoirs being coupled between the mobile phase drive and the separation unit to further having another one of the plurality of sample reservoirs being coupled between the mobile phase drive and the separation unit.

3. The sample dispatcher of claim 2 , wherein the sample dispatcher is configured for at least one of:
diluting the respective sample fluid portion with the mobile phase during the dilution state;
maintaining the dilution state at least for a time interval sufficient for displacement of at least a part of the content of the plurality of sample reservoirs, coupled between the mobile phase drive and the separation unit, simultaneously into a common fluid conduit;
changing composition of a respective sample fluid portion that has been stored in one of the plurality of sample reservoirs by mixing with the content displaced out of another of the plurality of sample reservoirs into a common conduit;
pressure relief of a respective sample reservoir after having been coupled between and subsequently decoupled from the mobile phase drive and the separation unit and before being coupled for receiving and temporarily storing a respective sample fluid portion or at least a part thereof;
precompressing a respective sample reservoir before being coupled between the mobile phase drive and the separation unit and after being coupled for receiving and temporarily storing a respective sample fluid portion or at least a part thereof.

4. The sample dispatcher of claim 1 , wherein:
the sample dispatcher is configured so that essentially at any point in the time at least one of the plurality of sample reservoirs, either alone or in a parallel combination, is coupled between the mobile phase drive and the separation unit.

5. The sample dispatcher of claim 1 , wherein:
the sample dispatcher receives the plurality of portions of one or more sample fluids and is configured for loading a respective sample fluid portion into at least one of the plurality of sample reservoirs.

6. The sample dispatcher of claim 1 , comprising:
a switching valve having a plurality of ports and a plurality of flow couplers, wherein:
each port is configured for coupling a fluid flow path to the switching valve;
each flow coupler is configured for fluidically coupling between at least two of the ports;
the switching valve can be selectively operated between a plurality of different states;
in each different state at least one of the flow couplers is fluidically coupling to a different port;
the mobile phase drive is coupled to a first port of the plurality of ports, the separation unit is coupled to a second port of the plurality of ports, a flow path for receiving the plurality of portions of the one or more sample fluids is coupled to a third port of the plurality of ports, the first sample reservoir is coupled to a forth and to a fifth port of the plurality of ports, and the second sample reservoir is coupled to a sixth and to a seventh port of the plurality of ports; and
a first one of the flow couplers is configured for coupling between the second, fifth and the sixth ports, and a second one of the flow couplers is configured for coupling between the first, fourth and seventh ports, so that at least two of the plurality of sample reservoirs are coupled in parallel between the mobile phase drive and the separation unit.

7. The sample dispatcher of claim 6 , wherein:
a first subset of the plurality of ports is arranged along an inner circle;
a second subset of the plurality of ports is arranged along an outer circle having larger diameter than the inner circle;
a first subset of the plurality of flow couplers is configured to couple between ports of the first subset of the plurality of ports; and
a second subset of the plurality of flow couplers is configured to couple between ports of the second subset of the plurality of ports.

8. The sample dispatcher of claim 7 , wherein:
the first subset of the plurality of ports has four ports;
the second subset of the plurality of ports has four ports;
the first subset of the plurality of flow couplers has two flow couplers; and
the second subset of the plurality of flow couplers has two flow couplers.

9. The sample dispatcher of claim 8 , wherein:
the plurality of ports are arranged symmetrically; and
one of the flow couplers in each of the first and second subsets of the plurality of flow couplers is longer than the other in the same subset.

10. A separation system for separating sample fluid compounds, the separation system comprising:
a first mobile phase drive configured to drive a first mobile phase through the separation system;
a sample providing apparatus being configured to provide a plurality of portions of one or more sample fluids;
a sample dispatcher of claim 1 being coupled to the first mobile phase drive and to the sample providing apparatus, and being configured to introduce the provided sample fluid portions into a flow of the first mobile phase; and
a first separation unit configured for separating compounds of the sample fluid in the first mobile phase,
wherein the sample dispatcher is configured to load a respective sample fluid portion into at least one of the one or more sample reservoirs.

11. The separation system of claim 10 , further comprising at least one of:
a detector configured to detect separated compounds of the sample fluid;
a collection unit configured to collect separated compounds of the sample fluid;
a data processing unit configured to process data received from the separation system;
a degassing apparatus for degassing the mobile phase.

12. The separation system of claim 10 , wherein the sample providing apparatus comprises:
a second mobile phase drive configured to drive a second mobile phase through a separation subsystem; and
a second separation unit configured for separating compounds of the sample fluid in the second mobile phase,
wherein at least a portion of the separated compounds are provided to the sample dispatcher as the plurality of portions of one or more sample fluids.

13. A method of individually introducing a plurality of portions of one or more sample fluids into a flow of a mobile phase of a separation system configured for separating compounds of the sample fluids, wherein the separation system comprises: a mobile phase drive configured for driving the mobile phase through a

Assignees

• Agilent Technologies Inc

Inventors

• Witt Klaus
• Shoykhet Konstantin
• Berndt Manfred

Classifications

• G01N30/20 Primary

  using a sampling valve · CPC title

• G01N30/465

  with specially adapted interfaces between the columns · CPC title

• G01N2030/027

  Liquid chromatography · CPC title

• G01N2030/202

  rotary valves · CPC title

• G01N2030/208

  with more than one cavity · CPC title