Method and device for determining intracellular and/or extracellular, in particular macromolecular fractions of fluids, preferably of body fluids of living organisms

Patent metadata

Field	Value
Publication number	US-9823207-B2
Application number	US-201113697132-A
Country	US
Kind code	B2
Filing date	May 11, 2011
Priority date	May 11, 2010
Publication date	Nov 21, 2017
Grant date	Nov 21, 2017

Abstract

Method and device according to the method for determining intracellular and/or extracellular, in particular macromolecular fractions of fluids, preferably of body fluids of living organisms, with the steps:
coupling-in a measurement signal through an electrically non-conductive wall into the fluid to be measured; coupling-out an electrical measurement value that is thereby generated in the fluid to be measured; detecting the coupled-out electrical measurement value at a plurality of different frequencies of the electrical measurement signal; determining the intracellular and/or extracellular, in particular macromolecular fractions of the fluid to be measured by means of evaluation of the detected electrical measurement value at a plurality of frequencies of the measurement signal.

First claim

The invention claimed is:

1. Method for determining intracellular and extracellular fractions of a fluid using a device, wherein the device comprises
a coupling-in device,
a measurement signal generator, whose measurement signal can be capacitively and inductively coupled in, simultaneously or alternatively, via the coupling-in device through an electrically non-conductive wall into the fluid to be measured,
a coupling-out device by which an electrical measurement value that is generated by the coupled-in measurement signal in the fluid to be measured can be capacitively and inductively coupled out, simultaneously or alternatively, through the electrically non-conductive wall,
a detecting device configured for detecting the coupled-out electrical measurement value, and
an evaluation device configured for determining the intracellular and extracellular fractions of the fluid by calculation from a plurality of measurement values generated at different frequencies of the measurement signal that are detected by the detecting device,
wherein the inductive coupling-in is employed for frequencies of the measurement signal lower than the frequencies of the measurement signal for which the capacitive coupling-in is employed, and
wherein the inductive coupling-in is supplied by at least one coupling coil,

the method comprising the steps of:
coupling-in with the coupling-in device a measurement signal generated by the measurement signal generator through the electrically non-conductive wall into the fluid to be measured;
coupling-out with the coupling-out device an electrical measurement value that is thereby generated in the fluid to be measured;
detecting with the detecting device the coupled-out electrical measurement value at a plurality of frequencies of the measurement signal; and
determining with the evaluation device at least one of the intracellular and extracellular fractions of the fluid to be measured by evaluation of the detected electrical measurement value at a plurality of frequencies of the measurement signal.

2. Method according to claim 1 , characterized in that the measurement signal is at least one of an electrical, magnetic, and electromagnetic alternating field, wherein the frequency of the alternating field is variable and modulatable.

3. Method according to claim 1 , characterized in that each of the coupling-in of the measurement signal into the fluid to be measured, and the coupling-out of the electrical measurement value thereby generated in the fluid to be measured, takes place capacitively, inductively, or capacitively and inductively.

4. Method according to claim 1 , characterized in that at least a portion of the measurement signal is capacitively coupled into the fluid to be measured, and at least a portion of the electrical measurement value thereby generated in the fluid to be measured is capacitively coupled out.

5. Method according to claim 1 , characterized in that the coupling-in device has at least one pair of coupling-in electrodes, and the coupling-out device has at least one pair of coupling-out electrodes, and characterized in that the capacitive coupling-in of the measurement signal into the fluid to be measured takes place by using the pair of coupling-in electrodes, and the coupling-out of the electrical measurement value thereby generated in the fluid to be measured takes place by using the pair of coupling-out electrodes as four-point measurement.

6. Method according to claim 1 , characterized in that each of the coupling-in of the measurement signal into the fluid to be measured, and the coupling-out of the electrical measurement value thereby generated in the fluid to be measured, takes place capacitively, inductively, or capacitively and inductively.

7. Method according to claim 1 , characterized in that the determining at least one of the intracellular and extracellular fractions of the fluid comprises detecting electrical impedance of the fluid to be measured at a plurality of different frequencies of the measurement signal according to amplitude and phase.

8. Method according to claim 1 , characterized in that the determining at least one of the intracellular and extracellular fractions of the fluid comprises evaluation on the basis of the Cole model.

9. Device configured for determining intracellular and extracellular fractions of a fluid comprising
a coupling-in device,
a measurement signal generator, whose measurement signal can be capacitively and inductively coupled in, simultaneously or alternatively, via the coupling-in device through an electrically non-conductive wall into the fluid to be measured,
a coupling-out device by which an electrical measurement value that is generated by the coupled-in measurement signal in the fluid to be measured can be capacitively and inductively coupled out, simultaneously or alternatively, through the electrically non-conductive wall,
a detecting device configured for detecting the coupled-out electrical measurement value,
an evaluation device configured for determining the intracellular and extracellular fractions of the fluid by calculation from a plurality of measurement values generated at different frequencies of the measurement signal that are detected by the detecting device,

wherein the inductive coupling-in is employed for frequencies of the measurement signal lower than the frequencies of the measurement signal for which the capacitive coupling-in is employed, and
wherein the inductive coupling-in is supplied by at least one coupling coil.

10. Device according to claim 9 , characterized in that the coupling-in device and/or the coupling-out device have flat electrodes for capacitive coupling-in of the measurement signal and for capacitive coupling-out of the electrical measurement value that is generated in the fluid to be measured and/or at least one coil for the inductive coupling-in of the measurement signal and/or at least one sensor for the measurement of a magnetic field influenced by an electrical value generated in the fluid to be measured.

11. Device according to claim 9 , characterized in that the coupling-in device has at least one pair of coupling-in electrodes, and the coupling-out device has at least one pair of coupling-out electrodes, wherein the coupling-out electrodes are substantially disposed between the coupling-in electrodes.

12. Device according to claim 11 , characterized in that the coupling-in and coupling-out electrodes are disposed on the outside of a fluid line which conveys the fluid with the intracellular and extracellular fractions that are to be determined.

13. Device according to claim 9 , characterized in that the detecting device has a device for detecting an impedance according to amplitude and phase.

14. Device according to claim 9 , characterized in that the coupled-out electrical measurement values can be evaluated in the evaluation device on the basis of the Cole model.

15. Dialysis machine comprising a dialyser and at least one device according to claim 9 , wherein the dialysis machine is configured to be controlled or regulated depending on the determined intracellular and extracellular fractions of the fluid.

16. Dialysis machine according to claim 15 , characterized in that the device is disposed downstream of the dialyzer and is configured for determination of a water fraction, which controls or regulates a transmembrane pressure in the dialysis device.

17. Dialysis machine according to claim 15 , characterized in that the device is configured for detection of air bubbles and/or detection of hemolysis, and if

Assignees

• Wieskotten Sebastian
• Groeber Tobias
• Chamney Paul
• Wabel Peter
• Moissl Ulrich
• Fresenius Medical Care Deutschland Gmbh

Inventors

• Wieskotten Sebastian
• Groeber Tobias
• Chamney Paul
• Wabel Peter
• Moissl Ulrich

Classifications

• A61M2205/3317

  Electromagnetic, inductive or dielectric measuring means · CPC title

• A61M1/3669

  Electrical impedance measurement of body fluids; transducers specially adapted therefor · CPC title

• G01N33/48735

  Investigating suspensions of cells, e.g. measuring microbe concentration (by chemical means C12Q1/04; colony counters C12M1/34; concentration of particle suspensions in general G01N15/06) · CPC title

• G01N27/00 Primary

  Investigating or analysing materials by the use of electric, electrochemical, or magnetic means (G01N3/00 – G01N25/00 take precedence; measurement or testing of electric or magnetic variables or of electric or magnetic properties of materials G01R) · CPC title

• A61M1/3626 Primary

  Gas bubble detectors · CPC title