Processing biomass
US-2015353974-A1 · Dec 10, 2015 · US
Method for producing an L-glutamic acid and L-aspartic acid using a recombinant microorganism having enhanced expression of a ybjL protein
US9822385B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9822385-B2 |
| Application number | US-201514723776-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 28, 2015 |
| Priority date | Apr 17, 2007 |
| Publication date | Nov 21, 2017 |
| Grant date | Nov 21, 2017 |
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- Title
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Abstract
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An acidic substance having a carboxyl group is produced by culturing in a medium a microorganism which has been modified to enhance expression of the ybjL gene, and collecting the acidic substance having a carboxyl group from the medium.
First claim
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The invention claimed is: 1. A method for producing an acidic substance having a carboxyl group comprising culturing in a medium a microorganism, having an ability to produce the acidic substance having a carboxyl group, to produce and accumulate the acidic substance having a carboxyl group in the medium, collecting the acidic substance having a carboxyl group from the medium, wherein the acidic substance is L-glutamic acid and/or L-aspartic acid, wherein the microorganism has been modified to enhance expression of an ybjL gene, and wherein the ybjL gene encodes a protein selected from the group consisting of: (A) a protein comprising the amino acid sequence of SEQ ID NO: 2; and (B) a protein comprising a variant of the amino acid sequence of SEQ ID NO: 2, wherein one to 5 amino acid residues are substituted, deleted, inserted or added, and wherein, upon expression, said variant improves the ability of the microorganism to produce the acidic substance having a carboxyl group. 2. The method according to claim 1 , wherein said enhanced expression is obtained by a method selected from the group consisting of: A) increasing copy number of the ybjL gene, B) modifying an expression control sequence of the ybjL gene, and C) combinations thereof. 3. The method according to claim 1 , wherein the microorganism is a bacterium belonging to the family Enterobacteriaceae. 4. The method according to claim 3 , wherein the bacterium belongs to a genus selected from the group consisting of Escherichia, Enterobacter, Raoultella, Pantoea , and Klebsiella. 5. The method according to claim 1 , wherein the microorganism is a rumen bacterium. 6. The method according to claim 5 , wherein the microorganism is Mannheimia.
Assignees
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Classifications
having keto groups, e.g. 2-ketoglutaric acid · CPC title
Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid · CPC title
from bacteria · CPC title
- C12P13/14Primary
Glutamic acid; Glutamine · CPC title
Tricarboxylic acids, e.g. citric acid · CPC title
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- What does patent US9822385B2 cover?
- An acidic substance having a carboxyl group is produced by culturing in a medium a microorganism which has been modified to enhance expression of the ybjL gene, and collecting the acidic substance having a carboxyl group from the medium.
- Who is the assignee on this patent?
- Ajinomoto Kk
- What technology area does this patent fall under?
- Primary CPC classification C12P13/14. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Nov 21 2017 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).