Auto-inducible expression system
US-10106801-B2 · Oct 23, 2018 · US
T7 expression system, method for its production and use thereof for producing recombinant proteins
US9822348B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9822348-B2 |
| Application number | US-201515316232-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 17, 2015 |
| Priority date | Jul 1, 2014 |
| Publication date | Nov 21, 2017 |
| Grant date | Nov 21, 2017 |
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- Title
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Abstract
Official abstract text for this publication.
The invention relates to an expression system comprising a prokaryotic cell which contains a polynucleotide construct that encodes a T7 RNA polymerase under the control of a T7 promoter and under the control of an inducible promoter. The invention also relates to an expression vector which contains a gene that encodes a protein to be expressed under the control of a T7 promoter, characterized in that said expression vector comprises a plasmid stabilization system.
First claim
Opening claim text (preview).
The invention claimed is: 1. An expression system comprising a prokaryotic cell containing a nucleotide construct coding for a T7 RNA polymerase under control of a T7 promoter and under control of an inducible promoter, and an expression vector, which contains a gene encoding a protein to be expressed under control of the T7 promoter, wherein: (a) the prokaryotic cell contains no lambda phage DNA, (b) the prokaryotic cell is a cell of an Escherichia coli strain with the T7 RNA polymerase gene in a genome thereof, and (c) the expression vector contains a plasmid stabilization system. 2. The expression system as claimed in claim 1 , wherein the plasmid stabilization system is a member selected from the group consisting of multimer resolution systems (mrs), partitioning systems (par) and postsegregational killing systems (PSK). 3. An expression system comprising a prokaryotic cell containing a nucleotide construct coding for a T7 RNA polymerase under control of a T7 Promoter and under control of an inducible promoter, and an expression vector, which contains a gene encoding a protein to be expressed under control of the T7 promoter, wherein: (a) the prokaryotic cell contains no lambda phage DNA, (b) the expression vector contains a plasmid stabilization system and (c) the nucleotide construct comprises SEQ ID NO: 1. 4. A method for producing a recombinant protein, said method comprising: providing an expression system as claimed in claim 1 , fermenting the expression system under antibiotic-free conditions which are suitable for expression of the T7 RNA polymerase and expression of the recombinant protein, expressing the recombinant protein, and isolating the recombinant protein. 5. The method as claimed in claim 4 , wherein a targeted integration of the T7 RNA polymerase under control of the inducible promoter is effected into a proBA sequence of E. coli. 6. The expression system as claimed in claim 3 , wherein a targeted integration of the T7 RNA polymerase under control of the inducible promoter is effected into a proBA sequence of E. coli. 7. A method for producing a recombinant protein, said method comprising: providing an expression system as claimed in claim 3 , fermenting the expression system under antibiotic-free conditions which are suitable for expression of the T7 RNA polymerase and expression of the recombinant protein, expressing the recombinant protein, and isolating the recombinant protein.
Assignees
Inventors
Classifications
Expression systems using phage (lambda) regulatory sequences · CPC title
- C12N15/72Primary
Expression systems using regulatory sequences derived from the lac-operon · CPC title
- C12N9/1247Primary
DNA-directed RNA polymerase (2.7.7.6) · CPC title
- C12N15/68Primary
Stabilisation of the vector · CPC title
DNA-directed RNA polymerase (2.7.7.6) · CPC title
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Frequently asked questions
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- What does patent US9822348B2 cover?
- The invention relates to an expression system comprising a prokaryotic cell which contains a polynucleotide construct that encodes a T7 RNA polymerase under the control of a T7 promoter and under the control of an inducible promoter. The invention also relates to an expression vector which contains a gene that encodes a protein to be expressed under the control of a T7 promoter, characterized i…
- Who is the assignee on this patent?
- Wacker Chemie Ag
- What technology area does this patent fall under?
- Primary CPC classification C12N15/72. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Nov 21 2017 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).