Immune modulator for immunotherapy and vaccine formulation

Patent metadata

Field	Value
Publication number	US-9822154-B2
Application number	US-201314652078-A
Country	US
Kind code	B2
Filing date	Dec 14, 2013
Priority date	Dec 14, 2012
Publication date	Nov 21, 2017
Grant date	Nov 21, 2017

Abstract

A recombinant Flagrp170 protein and pharmaceutical compositions comprising a Flagrp170 protein and related molecules encoding same, and cells presenting such a protein are provided. The Flagrp170 protein comprises an NF- K B-activating domain of Flagellin and an ATP-binding domain truncated Grp170. The pharmaceutical compositions of the invention can be used for the treatment or prevention of cancer or infectious disease.

First claim

What is claimed is:

1. A polypeptide produced by the method of:
culturing a host cell comprising a fusion construct operably linked to an expression control sequence under conditions suitable for production of a protein encoded by the fusion construct, and
recovering the protein,
wherein the fusion construct comprises a polynucleotide encoding an NF-κB-activating domain of Flagellin in operable linkage with a polynucleotide encoding an ATP-binding domain truncated Grp170, and
wherein the NF-κB-activating domain of Flagellin comprises N-terminal amino acids 2-175 and C-terminal amino acids 402-495 of SEQ ID NO: 8, and the ATP-binding domain truncated Grp170 consists of amino acids 431-994 of SEQ ID NO: 2 or 3.

2. A polypeptide comprising an NF-κB-activating domain of Flagellin fused with a polynucleotide encoding an ATP-binding domain truncated Grp170, wherein the NF-κB-activating domain of Flagellin comprises N-terminal amino acids 2-175 and C-terminal amino acids 402-495 of SEQ ID NO: 8, and the ATP-binding domain truncated Grp170 consists of amino acids 431-994 of SEQ ID NO: 2 or 3.

3. An immunogenic composition comprising the polypeptide of claim 2 .

4. The polypeptide of claim 2 , which has the amino acid sequence of SEQ ID NO: 1.

5. The composition of claim 3 , wherein the polypeptide is non-covalently complexed with an additional immunogenic polypeptide.

Assignees

• Univ Virginia Commonwealth
• Health Research Inc

Inventors

• Wang Xiang-Yang
• Yu Xiaofei
• Subjeck John R

Classifications

• C07K2319/00

  Fusion polypeptide · CPC title

• C07K14/47 Primary

  from mammals · CPC title

• A61K2039/70

  Multivalent vaccine · CPC title

• A61K2039/572

  cytotoxic response · CPC title

• C07K14/255

  Salmonella (G) · CPC title