Method for using nanodiamonds to detect nearby magnetic nanoparticles
US-2015377865-A1 · Dec 31, 2015 · US
US9822151B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9822151-B2 |
| Application number | US-201615155695-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 16, 2016 |
| Priority date | May 19, 2011 |
| Publication date | Nov 21, 2017 |
| Grant date | Nov 21, 2017 |
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This disclosure relates to graphene derivatives, as well as related devices including graphene derivatives and methods of using graphene derivatives.
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What is claimed is: 1. A graphene derivative consisting of graphene oxide and a protein selected from the group consisting of Protein A, Protein G, an E72G3 protein, and their derivatives wherein the protein is covalently bonded to graphene oxide by an amide group and, optionally, a C 1 -C 20 alkylene spacer group. 2. The graphene derivative of claim 1 , wherein the protein is Protein A. 3. The graphene derivative of claim 1 , wherein the protein is a derivative of Protein A. 4. The graphene derivative of claim 3 , wherein the derivative of protein A is His-tagged Protein A. 5. A method of making a graphene derivative consisting of graphene oxide and a protein selected from the group consisting of Protein A, Protein G, an E72G3 protein, and their derivatives wherein the protein is covalently bonded to graphene oxide by an amide group comprising: (1) providing graphene oxide; (2) optionally, activating the carboxyl groups on the graphene oxide; (3) providing a protein selected from the group consisting of Protein A, Protein G, and an E72G3 protein; and (4) reacting the graphene oxide with the primary amine groups on the protein to covalently bind the protein to the graphene core by an amide group. 6. The method of claim 5 , wherein the carboxyl groups on the graphene oxide are activated by N-hydroxysuccinimide and N-ethyl-N′-(3-dimethylaminopropyl)-carbodiimide.
the carrier being inorganic · CPC title
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by electrolysis of water · CPC title
Affinity chromatography or related techniques based upon selective absorption processes · CPC title
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