Method of manufacturing hybrid scaffold and hybrid scaffold manufactured by the same

Patent metadata

Field	Value
Publication number	US-9821500-B2
Application number	US-201514590052-A
Country	US
Kind code	B2
Filing date	Jan 6, 2015
Priority date	Feb 18, 2014
Publication date	Nov 21, 2017
Grant date	Nov 21, 2017

Abstract

In an apparatus for manufacturing a hybrid scaffold, a first strand having bin compatible polymer and a second strand having a mixture of bio compatible material and cells alternate with each other. Thus, mechanical strength of the hybrid scaffold is improved, and the cells uniformly grow among entire region of the scaffold. Furthermore, diameters of the first and second strands and interval between the first and second strands are precisely controlled. Thus, the hybrid scaffold is precisely manufactured according to a scaffold design.

First claim

What is claimed is:

1. A method for manufacturing a hybrid scaffold, by using:
a first dispensing head including a first nozzle part dispensing first bio material supplied from a first material storing part,
a second dispensing head including a second nozzle part dispensing second bio material supplied from a second material storing part, and
a collecting member disposed under the first and second dispensing heads,
the method comprising:
a) preparing by
storing the first bio material being bio compatible polymer in the first material storing part, and
storing the second bio material being a mixture of bio compatible material and cells in the second material storing part;

b) firstly dispensing by controlling a position of the first dispensing head using a dispensing position controller to dispense liquefied or melted said bio compatible polymer on the collecting member, to form a first strand;
c) secondly dispensing by controlling a position of the second dispensing head using the dispensing position controller to dispense the mixture of the bio compatible material and the cells on the collecting member, to form a second strand; and
d) forming the hybrid scaffold by alternately disposing the first and second strands,
wherein the bio compatible polymer is one selected from the group consisting of poly-caprolactone (PCL), poly lactide (PLA), poly glycolide (PGA), poly lactide-co-glycolide (PLGA), polydioxanone (PDO) and poly-L-lactic acid (PLLA),
wherein the bio compatible material is one selected from the group consisting of protein and hydrogel,
wherein the first dispensing head further comprises a first piston and a first heating part,
the first piston pressing the first bio material stored in the first material storing part so that the first bio material is dispensed through the first nozzle part, and
the first heating part including a heating coil around an external surface of the first material storing part, the heating coil liquefying or melting the bio compatible polymer stored in the first material storing part, the liquefied bio compatible polymer being dispensed through first nozzle part,
wherein printing pressures and printing speeds of the first and second dispensing heads and diameters of the first and second nozzle parts are controlled based on a size of pores in the hybrid scaffold and a diameter of each of the first and second strands,
wherein the second dispensing head further comprises a second piston, an external housing and a screw part,
the second piston pressing the second bio material stored in the second material storing part,
the external housing including an inlet and an outlet, the second bio material stored in the second material storing part flowing in through the inlet, the second bio material flowing out through the outlet,
the screw part rotationally mounted in the external housing, the screw part including a screw connected between the inlet and the outlet, and
the second nozzle part connected to the outlet, the second bio material being dispensed through the second nozzle part,
wherein a rotational frequency of the screw part, through which the mixture of the bio compatible material and the cells is dispensed on the collecting member in the secondly dispensing, is about 1 rpm to about 100 rpm.

2. The method of claim 1 , wherein the preparing further comprises selecting the diameters of the first and second nozzle parts based on the size of the pores in the hybrid scaffold and a size of each of the first and second strands.

3. The method of claim 1 , wherein the firstly dispensing further comprises dispensing the bio compatible polymer to be printed at the printing speed of about 30 mm/min to about 600 mm/min and the printing pressure of about 10 kPa to about 1,000 kPa.

4. The method of claim 1 , wherein the secondly dispensing further comprises dispensing the mixture of the bio compatible material and the cells to be printed at the printing speed of about 30 mm/min to about 300 mm/min and the printing pressure of about 10 kPa to about 400 kPa.

5. The method of claim 1 , wherein the second material storing part comprises a plurality of material storages.

6. The method of claim 5 , wherein the second material storing part comprises:
a first material storage; and
a second material storage.

7. The method of claim 6 , wherein the second material storing part further comprises:
a third material storage including a solidifying agent or a bio material having cells different from the first material storage or the second material storage.

Assignees

• Korea Inst Mach & Materials

Inventors

• Lee Junhee
• Park Sua
• Kim Wan-Doo

Classifications

• B29C48/17

  the components having different colours · CPC title

• B29C2948/92514

  Pressure · CPC title

• B29C48/92

  Measuring, controlling or regulating · CPC title

• B29C2948/92704

  Temperature · CPC title

• B29C2948/9258

  Velocity · CPC title