Two-stage microfluidic device for acoustic particle manipulation and methods of separation

Patent metadata

Field	Value
Publication number	US-9821310-B2
Application number	US-201214007483-A
Country	US
Kind code	B2
Filing date	Mar 30, 2012
Priority date	Mar 31, 2011
Publication date	Nov 21, 2017
Grant date	Nov 21, 2017

Abstract

Exemplary embodiments of the present disclosure provide for two-stage microfluidic devices using surface acoustic waves, methods of use thereof, methods of making, methods of focusing and separating particles, and the like.

First claim

What is claimed is:

1. A method of separating particles, comprising:
flowing a plurality of particles, using one singular flow, into a first portion of a single microfluidic channel, wherein the flow is a non-sheath flow,
focusing the particles to a first area of the first channel using an interference of a first pair of surface acoustic waves,
flowing the plurality of particles along the first area into a second portion of the single microfluidic channel, wherein the second portion is in fluidic communication with the first channel, and
separating the plurality of particles along a length of the second portion using an interference of a second pair of surface acoustic waves, wherein separating includes separating the plurality of particles based on the volume, density, and compressibility of the particles as the particles travel the length of the second portion of the single microfluidic channel.

2. The method of claim 1 , wherein the first area is at a center area of the first portion of the single microfluidic channel.

3. The method of claim 1 , further generating a first pair of surface acoustic waves using a first pair of surface acoustic wave generators positioned on opposite sides of the first portion of the single microfluidic channel, wherein the first pair of surface acoustic waves interfere with one another to form a periodic distribution of one or more pressure nodes and anti-nodes on the first portion of the single microfluidic channel.

4. The method of claim 1 , wherein the first pair of surface acoustic wave generators produce a single pressure node.

5. The method of claim 1 , wherein first portion of the single microfluidic channel is interfaced with the second portion of the single microfluidic channel so that the center area of the first portion of the single microfluidic channel is ata center area of the second portion of the single microfluidic channel.

6. The method of claim 1 , further generating a second pair of surface acoustic waves using a second pair of surface acoustic wave generators positioned on opposite sides of the second portion of the single microfluidic channel, wherein the second pair of surface acoustic waves interfere with one another to form a periodic distribution of one or more pressure nodes and anti-nodes on the second portion of the single microfluidic channel.

7. The method of claim 1 , wherein the interference of the surface acoustic waves generated by the second pair of surface acoustic wave generators produce two pressure nodes.

Assignees

• Guldiken Rasim Oytun
• Jo Myeong Chan
• Zhe Jiang
• Univ Akron

Inventors

• Guldiken Rasim Oytun
• Jo Myeong Chan
• Zhe Jiang

Classifications

• G01N33/575

  for cancer · CPC title

• G01N2015/142

  Acoustic or ultrasonic focussing · CPC title

• G01N33/5044

  involving specific cell types · CPC title

• G01N33/5005

  involving human or animal cells (immunoassay G01N33/56966; immunoassays of protozoa G01N33/56905; protozoa in screening assays C12Q1/025) · CPC title

• B01L2400/0436

  acoustic forces, e.g. surface acoustic waves [SAW] · CPC title