Preparative separation-purification system for capturing target components

Patent metadata

Field	Value
Publication number	US-9821250-B2
Application number	US-201313893108-A
Country	US
Kind code	B2
Filing date	May 13, 2013
Priority date	May 15, 2012
Publication date	Nov 21, 2017
Grant date	Nov 21, 2017

Abstract

In a preparative separation-purification system for passing a solution containing a target component through a trap column to capture the target component in the column, and for subsequently passing an eluting solvent through the column to elute the captured component and collect it in a container, a dilution passage is merged with a collection passage for sending an eluate from the outlet end of the trap column to the collection container, and a diluting liquid is intermittently introduced through the dilution passage into the collection passage. The diluting liquid lowers the concentration of the target component in the eluate and impedes the deposition of the target component. Thus, clogging of the passage due to the deposition of the target component eluted from the trap column is effectively prevented.

First claim

The invention claimed is:

1. A preparative separation-purification system comprising:
a) a supply passage for supplying an eluting solvent to one end of a trap column, the eluting solvent being a solvent having an eluting power on a target component captured in the trap column;
b) a collection passage for sending an eluate exiting another end of the trap column to a collection container for collecting the target component, the eluate being the eluting solvent containing the target component, the collection passage being a tube whose first end is connected via a joint to a base end of a needle detachable to the another end of the trap column, and whose second end is connected to a base end of an ejection nozzle movable directly above the collection container;
c) a diluting liquid supply passage, connected to the joint, for supplying into the collection passage a diluting liquid for lowering a concentration of the target component in the eluate; and
d) a liquid-sending system for alternately performing supply of the eluting solvent into the supply passage and supply of the diluting liquid into the diluting liquid supply passage.

2. The preparative separation-purification system according to claim 1 , wherein the liquid-sending system comprises:
e) a liquid-sending pump provided in the supply passage;
f) a passage selector, provided in the supply passage between the liquid-sending pump and the trap column, for switching the passage so that the liquid supplied through the liquid-sending pump is selectively sent to either the trap column or the diluting liquid supply passage; and
g) a controller for operating the passage selector so as to alternately perform supply of liquid to the trap column and supply of liquid to the diluting liquid supply passage.

3. The preparative separation-purification system according to claim 2 , wherein: an additional passage selector is provided on the upstream side of the liquid-sending pump in such a manner that either the eluting solvent or the diluting liquid will be selectively drawn by the liquid-sending pump by changing the setting of the additional passage selector.

4. The preparative separation-purification system according to claim 1 , wherein the liquid-sending system comprises:
h) a first liquid-sending pump provided in the supply passage;
i) a second liquid-sending pump provided in the diluting liquid supply passage; and
j) a controller for operating the first liquid-sending pump and the second liquid-sending pump so as to alternately perform the supply of the eluting solvent by the first liquid-sending pump and the supply of the diluting liquid by the second liquid-sending pump.

Assignees

• Shimadzu Corp

Inventors

• Yamazaki Tomoyuki
• Stasica Przemyslaw
• Boughtflower Bob

Classifications

• B01D15/245

  Adding materials to the effluents · CPC title

• B01D15/424 Primary

  Elution mode · CPC title