Hyaluronic acid-collagen matrices for dermal filling and volumizing applications

Patent metadata

Field	Value
Publication number	US-9821086-B2
Application number	US-201514962897-A
Country	US
Kind code	B2
Filing date	Dec 8, 2015
Priority date	Sep 6, 2011
Publication date	Nov 21, 2017
Grant date	Nov 21, 2017

Abstract

Hydrogels comprising a macromolecular matrix and water may be used for aesthetic fillers, for example, dermal fillers. The macromolecular matrix may include a crosslinked combination of hyaluronic acid and collagen.

First claim

What is claimed is:

1. A method of generating adipose tissue in a soft tissue of a human subject, the method comprising:
contacting a hydrogel composition with adipose tissue ex vivo to provide a hydrogel/adipose tissue mixture; and
subcutaneously injecting the mixture into the soft tissue of the subject;

wherein the hydrogel composition comprises:
(i) water; and
(ii) a hyaluronic acid crosslinked with a collagen via a plurality of crosslink units, wherein at least a portion of the crosslink units comprise an amide bond; and wherein
the hyaluronic acid concentration is from 6 mg/mL to 20 mg/mL;
the collagen concentration is from 3 mg/mL to 12 mg/mL;
the hyaluronic acid:collagen weight ratio is from 1:1 to 7:1; and
the hydrogel has a storage modulus value of between 850 Pa and 5,000 Pa;

thereby generating an additional amount of the adipose tissue in the soft tissue of the subject.

2. The method of claim 1 , wherein the collagen comprises collagen type I, collagen type III, or a combination thereof.

3. The method of claim 1 , wherein the collagen is collagen type I.

4. The method of claim 2 or 3 , wherein the collagen is derived from a human or porcine source.

5. The method of claim 1 , wherein the hyaluronic acid has a molecular weight of about 1,000,000 Da to about 5,000,000 Da.

6. The method of claim 1 , wherein the hyaluronic acid has a molecular weight of about 1,000,000 Da to about 3,000,000 Da.

7. The method of claim 1 , wherein the ratio of the hyaluronic acid to the collagen is 1:1 to 2:1.

8. The method of claim 1 , wherein the ratio of the hyaluronic acid to the collagen is 1:1.

9. The method of claim 1 , wherein the hydrogel has a storage modulus value of between 1,000 Pa and 5,000 Pa.

10. The method of claim 1 , wherein the hydrogel has a hyaluronic acid concentration of 12 mg/mL or 16 mg/mL.

11. The method of claim 1 , wherein the hydrogel has a collagen concentration of 6 mg/mL, 8 mg/mL or 12 mg/mL.

12. The method of claim 1 , wherein the hydrogel has a hyaluronic acid concentration of 12 mg/mL and a collagen concentration of 6 mg/m L.

13. The method of claim 1 , wherein the hydrogel has a hyaluronic acid concentration of 12 mg/mL and a collagen concentration of 12 mg/mL.

14. The method of claim 1 , wherein the hydrogel has a hyaluronic acid concentration of 16 mg/mL and a collagen concentration of 8 mg/m L.

Assignees

• Allergan Inc

Inventors

• Yu Xiaojie
• Manesis Nicholas J
• Pollock Jacob

Classifications

• A61K8/042

  Gels · CPC title

• A61Q19/08

  Anti-ageing preparations · CPC title

• A61L2400/06

  Flowable or injectable implant compositions · CPC title

• A61L27/26

  Mixtures of macromolecular compounds · CPC title

• A61L2430/34

  for soft tissue reconstruction · CPC title