Methods of controlling parasitic worms in animals

Patent metadata

Field	Value
Publication number	US-9821028-B2
Application number	US-201414329439-A
Country	US
Kind code	B2
Filing date	Jul 11, 2014
Priority date	Jul 11, 2014
Publication date	Nov 21, 2017
Grant date	Nov 21, 2017

Abstract

Disclosed herein are methods of controlling parasitic worms in herbivorous mammals and other species by orally administering an isolated antibody that specifically binds the interleukin-10 peptide or an interleukin-10 peptide. In the case of herbivorous mammals such as cattle, for example, administration of the anti-IL-10 antibodies increases weight gain and increases feed efficiency in the animals.

First claim

The invention claimed is:

1. A method of controlling parasitic worms having a lifecycle stage that includes the gastrointestinal tract of a herbivorous mammal, comprising
orally administering to the herbivorous mammal an effective amount of an isolated antibody that specifically binds an interleukin-10 peptide when orally administered to the herbivorous mammal,
wherein the interleukin-10 peptide is SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, or SEQ ID NO:20, and wherein the mammal is infected with or suspected of being infected with a parasitic worm having a lifecycle stage that includes the gastrointestinal tract of the herbivorous mammal.

2. The method of claim 1 , wherein the isolated antibody that specifically binds the interleukin-10 peptide is an egg yolk antibody.

3. The method of claim 1 , wherein the isolated antibody that specifically binds the interleukin-10 peptide is administered in a feed composition.

4. The method of claim 1 , wherein the herbivorous mammal is a bovine, an equine, an ovine, a caprine, a goat, a llama, an alpaca, a deer, an elk, or a pig.

5. The method of claim 1 , wherein the herbivorous mammal is a ruminant or pre-ruminant animal.

6. The method of claim 5 , wherein the ruminant or pre-ruminant animal is a bovine, and the parasitic worm is a Haemonchus species.

7. The method of claim 1 , wherein the effective amount of the isolated antibody that specifically binds an interleukin-10 peptide increases weight gain in the herbivorous mammal.

8. The method of claim 1 , wherein the effective amount of the isolated antibody that specifically binds an interleukin-10 peptide reduces egg shedding in the herbivorous mammal.

9. The method of claim 8 , wherein the effective amount of the isolated antibody that specifically binds an interleukin-10 peptide reduces reinfection by parasitic worms in the herbivorous mammal.

10. The method of claim 1 , wherein the herbivorous mammal is part of a population of mammals that are infected with parasitic worms that are resistant to one or more antihelminthic agents.

11. The method of claim 10 , wherein the antihelminthic agent is a benzimidizole, a hydropyrimidine, an imidazothiazole, or a macrocylic lactone.

12. The method of claim 1 , wherein administering the effective amount of the isolated antibody that specifically binds the interleukin-10 peptide decreases the likelihood of death of the mammal.

13. The method of claim 1 , wherein the isolated antibody that specifically binds the interleukin-10 peptide is orally administered daily for 2 weeks or less.

14. The method of claim 13 , wherein administration of the isolated antibody that specifically binds the interleukin-10 peptide is not resumed for at least 2 weeks after daily administration is ceased.

15. A method of increasing weight gain or increasing feed efficiency in a herbivorous mammal, comprising
orally administering to the herbivorous mammal an effective amount of an isolated antibody that specifically binds an interleukin-10 peptide when orally administered to the herbivorous mammal, wherein the herbivorous mammal is infected with or is suspected of being infected with a parasitic worm having a lifecycle stage that includes the gastrointestinal tract of the herbivorous mammal,
wherein the IL-10 peptide is SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, or SEQ ID NO: 20.

16. The method of claim 15 , wherein the isolated antibody that specifically binds the interleukin-10 peptide is an egg yolk antibody.

17. The method of claim 15 , wherein the isolated antibody that specifically binds the interleukin-10 peptide is administered in a feed composition.

18. The method of claim 15 , wherein the herbivorous mammal is a bovine, a horse, a sheep, a goat, a llama, an alpaca, a deer, an elk, or a pig.

19. The method of claim 15 , wherein the herbivorous mammal is a ruminant or pre-ruminant animal.

20. The method of claim 19 , wherein the ruminant or pre-ruminant animal is a bovine, and the pirasitic worm is a Haemonchus species.

21. The method of claim 15 , wherein the effective amount of the isolated antibody that specifically binds an interleukin-10 peptide reduces egg shedding in the herbivorous mammal.

22. The method of claim 15 , wherein the effective amount of the isolated antibody that specifically binds an interleukin-10 peptide reduces reinfection by parasitic worms in the herbivorous mammal.

23. The method of claim 15 , wherein the herbivorous mammal is part of a population of mammals that are infected with parasitic worms that are resistant to one or more antihelminthic agents.

24. The method of claim 23 , wherein the antihelminthic agent is a benzimidizole, a hydropyrimidine, an imidazothiazole, or a macrocylic lactone.

25. The method of claim 15 , wherein administering the effective amount of the isolated antibody that specifically binds the interleukin-10 peptide decreases the likelihood of death of the mammal.

26. The method of claim 15 , wherein the isolated antibody that specifically binds the interleukin-10 peptide is orally administered daily for 2 weeks or less.

27. The method of claim 26 , wherein administration of the isolated antibody that specifically binds the interleukin-10 peptide is not resumed for at least 2 weeks after daily administration is ceased.

28. A method of providing resistance to reinfection with a parasitic worm having lifecycle stage that includes the gastrointestinal tract of an herbivorous mammal, comprising orally administering to the herbivorous mammal an effective amount of an isolated antibody that specifically binds an interleukin-10 peptide when orally administered to the herbivorous mammal, wherein the herbivorous mammal was infected with the parasitic worm at the time of oral administration of the isolated antibody that specifically binds the interleukin-10 peptide, and wherein
the IL-10 peptide is SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, or SEQ ID NO: 20.

29. The method of claim 28 , wherein the isolated antibody that specifically binds the interleukin-10 peptide is an egg yolk antibody.

30. The method of claim 28 , wherein the isolated antibody that specifically binds the interleukin-10 peptide is administered in a feed composition.

31. The method of claim 28 , wherein the herbivorous mammal is a bovine, an equine, an ovine, a caprine, a goat, a llama, an alpaca, a deer, an elk, or a pig.

32. The method of claim 28 , wherein the herbivorous mammal is a ruminant or pre-ruminant animal.

33. The method of claim 32 , wherein the ruminant or pre-ruminant animal is a bovine, and the parasitic worm is a Haemonchus species.

34. The method of claim 28 , wherein the effective amount of the isolated antibody that specifically binds an interleukin-10 peptide increases weight gain in the herbivorous mammal.

Assignees

• Wisconsin Alumni Res Found

Inventors

• Cook Mark Eric
• Schaefer Daniel Meilahn
• Schaefer Mitchell Raymond
• Sand Jordan Marshall
• Smith Larry

Classifications

• C07K16/244

  Interleukins [IL] · CPC title

• A61K2039/505

  comprising antibodies · CPC title

• C07K2317/23

  from birds · CPC title

• A61K38/2066 Primary

  IL-10 · CPC title

• C07K2317/34

  Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues · CPC title