Tubular vascular graft and the preparation thereof

Patent metadata

Field	Value
Publication number	US-9820846-B2
Application number	US-201514686262-A
Country	US
Kind code	B2
Filing date	Apr 14, 2015
Priority date	Dec 12, 2014
Publication date	Nov 21, 2017
Grant date	Nov 21, 2017

Abstract

The present invention provides a reparation method of a tubular vascular graft, (a) immersing a tubular scaffold in a first light sensitivity gelatin solution, and irradiate the tubular scaffold by a first time period, to let surface of the tubular scaffold form a base layer; (b) immersing the tubular scaffold of the step (a) in a chitin gelatin solution, when the surface of the base layer form a film, then immersing the tubular scaffold into a sodium hydroxide solution to generate a middle layer of the surface of the base layer; (c) immersing the tubular scaffold of the step (b) in a second light sensitivity gelatin solution, wherein the second light sensitivity gelatin solution comprises a cell, the tubular scaffold is irradiated by a second time period to form a surface layer of the middle layer; (d) until the cell forms a tubular structure of the surface layer by the cell in the tubular scaffold of the step (c), heating the tubular scaffold by a temperature to solve the base layer into a solution, pulling out the tubular scaffold to get an artificial blood vessels.

First claim

What is claimed is:

1. A method of preparing a tubular vascular graft, which comprises the steps:
immersing a tubular scaffold in a first light sensitive gelatin solution, and irradiating the tubular scaffold by a first time period, to form a base layer on the surface of the tubular scaffold;
immersing the tubular scaffold of the step (a) in a chitin gelatin solution, when the surface of the base layer form a film, then immersing the tubular scaffold into a sodium hydroxide solution to generate a middle layer on the surface of the base layer;
immersing the tubular scaffold of the step (b) in a second light sensitivity gelatin solution, wherein the second light sensitivity gelatin solution comprises a plurality of cells, the tubular scaffold is irradiated by a second time period to form a surface layer on the surface of the middle layer;
until the cells grow as a tubular structure within the surface layer of the step (c), heating the tubular scaffold by a temperature to dissolve the base layer into a solution, removing the tubular scaffold to get an artificial blood vessel.

2. The method of claim 1 , wherein the first sensitive gelatin solution and the second light sensitivity gelatin solution are dissolved in a phosphate solution comprising 10% gelatin and 1% photoinitiator.

3. The method of in claim 2 , wherein the gelatin solution is prepared from a 10 g gelatin solution, a 100 mL phosphate solution, and the 2-methyl-2-propenoic acid anhydride.

4. The method of in claim 3 , wherein the gelatin is extracted from collagen in animal connective tissue.

5. The method of in claim 1 , wherein if the light is ultraviolet, the first time period is 10 seconds.

6. The method of in claim 1 , wherein if the chitin gelatin solution is 1% (W/V), the chitin is dissolved in a 1% acetic acid solution.

7. The method of in claim 1 , wherein if the light is ultraviolet, the second time period is 12 seconds.

8. The method of in claim 1 , wherein the tubular scaffold is a transparent heat conducting material with a low biological toxicity.

Assignees

• Univ Nat Tsing Hua

Inventors

• Tseng Fan-Gang
• Chu Shueh-Yao
• Chu Chiao-Wen

Classifications

• A61F2/062 Primary

  Apparatus for the production of blood vessels made from natural tissue or with layers of living cells · CPC title

• B05D2254/02

  Applying the material on the exterior of the tube · CPC title

• B05D1/18

  performed by dipping · CPC title

• B29D1/00

  Producing articles with screw-threads · CPC title