Systems and methods for processing and imaging of biological samples

Patent metadata

Field	Value
Publication number	US-9817221-B2
Application number	US-201314410890-A
Country	US
Kind code	B2
Filing date	Jun 26, 2013
Priority date	Jun 29, 2012
Publication date	Nov 14, 2017
Grant date	Nov 14, 2017

Abstract

A method for processing and imaging a first and second plurality of samples, comprising processing at least one sample from the first plurality of samples, imaging the at least one sample from the first plurality of samples, while being capable of simultaneously processing at least one sample from the second plurality of samples; and imaging the at least one processed sample from the second plurality of samples.

First claim

The invention claimed is:

1. A system for processing and imaging a plurality of samples, comprising:
a sample processing unit configured to process one or more of the plurality of samples;
an image acquisition unit operatively coupled to the sample processing unit; and
a sample conveyance unit configured to transport one or more of the plurality of samples to the sample processing unit, image acquisition unit, or both,
wherein the system is configured to process at least one sample from the plurality of samples while simultaneously imaging another sample from the plurality of samples.

2. The system of claim 1 , wherein the sample conveyance unit comprises:
a sample stage configured to receive the plurality of samples; and
a driving member configured to provide a translational movement, rotational movement, or both to the sample stage.

3. The system of claim 2 , wherein the sample stage comprises a continuous surface.

4. The system of claim 1 , wherein the image acquisition unit comprises one or more microscopes.

5. The system of claim 4 , wherein the sample conveyance unit is an integral part of the one or more microscopes.

6. The system of claim 1 , wherein the image acquisition unit is divided by color channel into two or more separate optical engines.

7. The system of claim 1 , wherein the image acquisition unit is divided by magnification factor into two or more separate optical engines.

8. The system of claim 1 , wherein the image acquisition unit is divided by acquisition numerical aperture in to two or more separate optical engine.

9. The system of claim 1 , wherein the image acquisition unit is divided by both color channel and magnification factor into two or more separate optical engines.

10. The system of claim 9 , wherein a sample is grouped into said first and second plurality of samples based on a scan area of each sample, as well as information about an assay, to achieve minimum time-to-completion grouping of samples.

11. An automated method for processing and imaging a first and second plurality of samples, comprising:
processing at least one sample from the first plurality of samples;
imaging the at least one sample from the first plurality of samples, while also being capable of simultaneously processing at least one sample from the second plurality of samples; and
imaging the at least one processed sample from the second plurality of samples,
wherein providing the first and second plurality of samples comprises disposing the first and second plurality of samples in two discrete locations on a sample stage.

12. The method of claim 11 , further comprising acquiring a low magnification image of each sample that relates scan area of each sample and grouping the samples into the first and second plurality of samples based on at least the scan area of each sample.

13. The method of claim 11 , further comprising imaging the first and second plurality of samples before any processing is performed to obtain background Images.

14. The method of claim 11 , further comprising imaging one of the first or the second plurality of samples, prior to processing, while the other set of sample is being processed, to establish background images.

15. An automated method for processing and imaging a first and second plurality of samples, comprising:
processing at least one sample from the first plurality of samples;
imaging the at least one sample from the first plurality of samples, while also being capable of simultaneously processing at least one sample from the second plurality of samples; and
imaging the at least one processed sample from the second plurality of samples,
wherein providing the first and second plurality of samples comprises disposing the first and second plurality of samples on a sample stage.

16. The method of claim 15 , wherein the sample stage is a stage of an imaging device.

Assignees

• Gen Electric

Inventors

• Corwin Alex David
• Filkins Robert John
• Xie Jun
• Chen Nannan
• Shaikh Kashan Ali

Classifications

• G02B21/008 Primary

  Details of detection or image processing, including general computer control · CPC title

• G01N1/30

  Staining; Impregnating {; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis} · CPC title

• G01N35/0092

  Scheduling · CPC title

• G01N35/0099

  comprising robots or similar manipulators (robots per se B25J) · CPC title

• G01N35/00029

  provided with flat sample substrates, e.g. slides (G01N35/028 takes precedence) · CPC title