Low cost optical high speed discrete measurement system

Patent metadata

Field	Value
Publication number	US-9817016-B1
Application number	US-201715672587-A
Country	US
Kind code	B1
Filing date	Aug 9, 2017
Priority date	May 29, 2013
Publication date	Nov 14, 2017
Grant date	Nov 14, 2017

Abstract

Systems and methods are provided for determining a velocity or an inflation rate of a droplet in a microfluidic channel. The droplet is exposed to two or more temporally separated flashes of light, each flash including light of one wavelength band, and imaged using a detector configured to distinguish light in the wavelength bands. Two or more images of the droplet are acquired, each corresponding to one of the flashes, and all within a single video frame or photographic exposure. The images can be processed separately and the position or size of the droplet in each image is calculated. A velocity or inflation rate is then determined by dividing the change in position or size by the amount of time allowed to pass between the flashes.

First claim

What is claimed is:

1. A system for determining an inflation rate of a droplet passing through a microfluidic channel, the system comprising:
a multicolor light source configured to emit light in two or more different wavelength bands, as temporally separated flashes, wherein each flash comprises light of only one of said wavelength bands;
a detector configured to detect and distinguish between light in said wavelength bands, and further configured to collect at least a first image and a second image of a droplet passing through the microfluidic channel at said different wavelength bands; and
the microfluidic channel disposed in a translucent material,
wherein the multicolor light source, the detector, and the microfluidic channel are positioned such that light emitted by the multicolor light source is incident upon the microfluidic channel, and such that a droplet passing through the microfluidic channel and illuminated by the multicolor light source is imaged on the detector, and
wherein the system is configured to calculate a change in size of the droplet between the first image and the second image by separating the first image from the second image in a video frame or a photographic exposure and determining a first diameter of the droplet in the first image and a second diameter of the droplet in the second image.

2. The system of claim 1 , wherein the multicolor light source is a multicolor LED.

3. The system of claim 2 , wherein the multicolor LED is a three-color LED.

4. The system of claim 1 , wherein the detector is configured to detect light in two or more color channels, each color channel corresponding to one of said wavelength bands.

5. The system of claim 1 , wherein the detector comprises a filter configured to pass light in at least one of said wavelength bands.

6. The system of claim 1 , further comprising a source aperture interposed between the multicolor light source and the microfluidic channel.

7. The system of claim 1 , further comprising a microscope interposed between the microfluidic channel and the detector, wherein the microscope comprises a first lens, a second lens, and an aperture stop interposed between the first lens and the second lens.

8. The system of claim 1 , further comprising a pressure source coupled to the microfluidic channel.

9. The system of claim 1 , wherein the system is further configured, in combination with calculating the change in size of the droplet, to determine a velocity of the droplet.

10. The system of claim 1 , wherein the system is further configured, in combination with determining a first diameter of the droplet in the first image and a second diameter of the droplet in the second image, to calculate a change in position of the droplet between the first image and the second image, determining a first centroid position of the droplet in the first image and a second centroid position of the droplet in the second image.

11. A system for determining a velocity of a droplet passing through a microfluidic channel, the system comprising:
a multicolor light source configured to emit light in two or more different wavelength bands, as temporally separated flashes, wherein each flash comprises light of only one of said wavelength bands;
a detector configured to detect and distinguish between light in said wavelength bands, and further configured to collect at least a first image and a second image of a droplet passing through the microfluidic channel at said different wavelength bands; and
the microfluidic channel disposed in a translucent material,
wherein the multicolor light source, the detector, and the microfluidic channel are positioned such that light emitted by the multicolor light source is incident upon the microfluidic channel, and such that a droplet passing through the microfluidic channel and illuminated by the multicolor light source is imaged on the detector, and
wherein the system is configured to calculate a change in position of the droplet between the first image and the second image by separating the first image from the second image in a video frame or a photographic exposure, determining a first centroid position and a first diameter of the droplet in the first image and a second centroid position and a second diameter of the droplet of the droplet in the second image.

12. The system of claim 11 , wherein the multicolor light source is a multicolor LED.

13. The system of claim 12 , wherein the multicolor LED is a three-color LED.

14. The system of claim 11 , wherein the detector is configured to detect light in two or more color channels, each color channel corresponding to one of said wavelength bands.

15. The system of claim 11 , wherein the detector comprises a filter configured to pass light in at least one of said wavelength bands.

16. The system of claim 11 , further comprising a source aperture interposed between the multicolor light source and the microfluidic channel.

17. The system of claim 11 , further comprising a microscope interposed between the microfluidic channel and the detector, wherein the microscope comprises a first lens, a second lens, and an aperture stop interposed between the first lens and the second lens.

18. The system of claim 11 , further comprising a pressure source coupled to the microfluidic channel.

19. The system of claim 11 , wherein the system is further configured, in combination with calculating the change in size of the droplet, to determine an inflation rate of the droplet.

20. The system of claim 11 , wherein the system is further configured to calculate a change in size of the droplet between the first image and the second image.

Assignees

• Bio Rad Laboratories Inc

Inventors

• Tracy David H
• Weber Aaron
• Stokes Peter

Classifications

• G01N15/1459

  the analysis being performed on a sample stream · CPC title

• B01L2400/0487

  fluid pressure, pneumatics · CPC title

• G01N2015/1493

  Particle size · CPC title

• B01L3/502784

  specially adapted for droplet or plug flow, e.g. digital microfluidics · CPC title

• B01L2200/143

  Quality control, feedback systems · CPC title