Antibody for detecting epithelial ovarian cancer marker and method for diagnosing epithelial ovarian cancer

Patent metadata

Field	Value
Publication number	US-9816996-B2
Application number	US-201214234783-A
Country	US
Kind code	B2
Filing date	Jul 26, 2012
Priority date	Jul 26, 2011
Publication date	Nov 14, 2017
Grant date	Nov 14, 2017

Abstract

The present invention provides an antibody capable of specifically recognizing and detecting the highly specific cancer marker with respect to the epithelial ovarian cancer, or a fragment of the antibody. The present invention provides an anti-β1,3-N-acetylglucosaminyltransferase 3 antibody for diagnosis of epithelial ovarian cancer, i.e., an antibody for detection of a glycosyltransferase β1,3-N-acetylglucosaminyltransferase 3 as an epithelial ovarian cancer marker. The antibody recognizes, as an epitope, a part of a polypeptide of the enzyme consisting of the amino acid sequence represented by SEQ ID NO: 1.

First claim

The invention claimed is:

1. A method for diagnosis of epithelial ovarian cancer comprising quantitatively detecting a β-1,3-N-acetylglucosaminyltransferase 3 polypeptide fragment and a β-1,6-N-acetylglucosaminyltransferase 5B polypeptide fragment present in a body fluid derived from a test subject, and
determining that the test subject is diagnosed to be likely to have epithelial ovarian cancer when the detection results of the β-1,3-N-acetylglucosaminyltransferase 3 polypeptide fragment and the β1,6-N-acetylglucosaminyltransferase 5B polypeptide fragment are greater than a predetermined cut-off value,
wherein the β-1,3-N-acetylglucosaminyltransferase 3 polypeptide fragment is detected using at least one antibody or antibody fragment selected from the group consisting of an antibody for detecting an epithelial ovarian cancer marker that recognizes as an epitope a part of a polypeptide consisting of the amino acid sequence of SEQ ID NO: 1; and a fragment of the antibody for detecting an epithelial ovarian cancer marker that recognizes as an epitope a part of a polypeptide consisting of the amino acid sequence of SEQ ID NO: 1, and
wherein the predetermined cut-off value is 95 percentile of the detection results of the β-1,3-N-acetylglucosaminyltransferase 3 polypeptide fragment and the β1,6-N-acetylglucosaminyltransferase 5B polypeptide fragment in body fluid derived from normal individuals or patients other than epithelial ovarian cancer, and wherein the antibody is produced by a hybridoma identified by International Accession No. FERM BP-11494 or FERM BP-11495.

2. The method for diagnosis of epithelial ovarian cancer of claim 1 , wherein the β-1,6-N-acetylglucosaminyltransferase 5B polypeptide fragment is detected using at least one antibody or antibody fragment selected from the group consisting of an anti-β-1,6-N-acetylglucosaminyltransferase 5B antibody recognizing as an epitope a part of a polypeptide consisting of the amino acid sequence of SEQ ID NO: 3, and a fragment of the anti-β-1,6-N-acetylglucosaminyltransferase 5B antibody, wherein the fragment specifically recognizes β-1,6-N-acetylglucosaminyltransferase 5B.

3. The method for diagnosis of epithelial ovarian cancer of claim 2 , wherein the anti-β-1,6-N-acetylglucosaminyltransferase 5B antibody is produced by a hybridoma identified by International Accession No. FERM BP-11496, FERM BP-11497, FERM BP-11498, or FERM BP-11499.

4. A monoclonal antibody produced by a hybridoma identified by International Accession No. FERM ABP-11494 or FERM ABP-11495.

5. A hybridoma identified by International Accession No. FERM ABP-11494.

6. A hybridoma identified by International Accession No. FERM ABP-11495.

Assignees

• Sogabe Maki
• Kubota Tomomi
• Togayachi Akira
• Ikehara Yuzuru
• Narimatsu Hisashi
• Sawaki Hiromichi
• Nakanishi Hayao
• Nakanishi Toru
• Aist

Inventors

• Sogabe Maki
• Kubota Tomomi
• Togayachi Akira
• Ikehara Yuzuru
• Narimatsu Hisashi
• Sawaki Hiromichi
• Nakanishi Hayao
• Nakanishi Toru

Classifications

• G01N33/57545 Primary

  of the ovaries · CPC title

• C07K16/40

  against enzymes · CPC title

• C07K16/3069

  Reproductive system, e.g. ovaria, uterus, testes, prostate · CPC title

• C12N5/163

  one of the fusion partners being a B or a T lymphocyte · CPC title

• G01N33/57449 Primary

  Physics · mapped topic