Method for treating a blood component containing sample

Patent metadata

Field	Value
Publication number	US-9816994-B2
Application number	US-201615161170-A
Country	US
Kind code	B2
Filing date	May 20, 2016
Priority date	Jun 20, 2012
Publication date	Nov 14, 2017
Grant date	Nov 14, 2017

Abstract

Provided is a treatment method for damaging an erythrocyte and a leukocyte while suppressing damage to cells other than blood cells present in blood. In an embodiment, the disclosure relates to a method for treating a sample containing blood components, the method including mixing a sample containing blood components with a surfactant A, where the surfactant A is a nonionic surfactant represented by General formula R 1 —O—(EO)n-R 2 (I).

First claim

What is claimed is:

1. A method for separating or detecting a tumor cell and/or a cancer cell in a sample containing blood components, the method comprising treating the sample by mixing the sample with a liquid containing a surfactant A, wherein no fixation agent is used, and the surfactant A is a nonionic surfactant represented by formula (I)
R 1 —O-(EO) n -R 2   (I)

wherein:
R 1 is a hydrocarbon group having a branched chain and having a carbon number in the range of 12 to 40,
EO is an oxyethylene group,
n is an average addition mole number of EO in the range of 23 to 50, and
R 2 is a hydrogen atom or a hydrocarbon group having a carbon number in the range of 1 to 3,
wherein the method eliminates erythrocytes and leukocytes present in the sample while suppressing damage to the tumor cell and/or the cancer cell.

2. The method according to claim 1 , wherein the surfactant A is a polyoxyethylene alkylene ether with an EO in a range of 23 to 35.

3. The method according to claim 1 , wherein the liquid containing the surfactant A satisfies a blood cell elimination effect as described in (1) below:
(1) preparing a control sample by diluting a predetermined amount of blood with distilled water so that an absorbance value measured by absorption photometry using a micro-plate reader and light of a wavelength of not less than 600 nm is not less than 1 and is less than 2; and
preparing a test sample from a liquid containing 2.5 to 5 w/v % of the surfactant A, so that the time for the absorbance value of the test sample measured by absorption photometry by light having a wavelength of not less than 600 nm to be decreased to half of the absorbance value of the control sample is not more than one hour.

Assignees

• Arkray Inc

Inventors

• Takagi Hidenori

Classifications

• G01N33/575 Primary

  for cancer · CPC title

• G01N1/28

  Preparing specimens for investigation {including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q}(mounting specimens on microscopic slides G02B21/34; means for supporting the objects or the materials to be analysed in electron microscopes H01J37/20 {; laboratory gas handling apparatus B01L5/00}) · CPC title

• G01N33/5002

  Partitioning blood components · CPC title

• G01N33/574 Primary

  Physics · mapped topic

• C08G65/26

  from cyclic ethers and other compounds · CPC title