Devices, method and system employable for lysing

Patent metadata

Field	Value
Publication number	US-9816085-B2
Application number	US-201314062722-A
Country	US
Kind code	B2
Filing date	Oct 24, 2013
Priority date	Oct 25, 2012
Publication date	Nov 14, 2017
Grant date	Nov 14, 2017

Abstract

A device configured for lysing a sample includes a holding element configured to hold an energy-transmitting element such that the holding element lies around the held part of the energy-transmitting element and envelops the held part of the energy-transmitting element and/or that the holding element lies against the held part of the energy-transmitting element. The holding element is further configured to cause lysis with the energy-transmitting element when the holding element is immersed into the sample to be subjected to lysis or comes into contact with the sample to be subjected to lysis. A method includes inserting the energy-transmitting element into the holding element. A system configured for lysing a sample by way of ultrasound includes the device and the energy transmitting element.

First claim

What is claimed is:

1. A device configured for lysing a sample to be subjected to lysis, comprising:
a lysis container for containing the sample to be subjected to lysis, the container having a cover to enclose the sample within the container; and
a holding element configured to hold an energy-transmitting element such that the holding element lies around a held part of the energy-transmitting element and envelops the held part of the energy-transmitting element,
the holding element further incorporated into one or more of the lysis container and the cover for the lysis container so that the holding element is at least partially immersed into the sample to be subjected to lysis,
wherein the energy-transmitting element is a sonotrode or a horn.

2. The device according to claim 1 , wherein the holding element is configured to lie directly around and against the held part of the energy-transmitting element.

3. The device according to claim 1 , wherein the holding element is configured as a membrane and/or is completely or at least partly elastic and deformable.

4. The device according to claim 3 , wherein the membrane has a thickness of between 10 μm to 400 μm, 50 μm to 1 mm, or 300 μm to 400 μm.

5. The device according to claim 1 , wherein the holding element is preformed in respect of the held part of the energy-transmitting element.

6. The device according to claim 3 , wherein the membrane has a thickness of between 10 μm and 1 mm.

7. The device according to claim 3 , wherein the holding element is configured so that the membrane arches in the direction of the energy-transmitting element and establishes a gap-free or substantially gap-free contact with the held part of the energy-transmitting element when pressure is applied to the sample within the container.

8. The device according to claim 1 , wherein one or more of:
the holding element is configured to at least partly hold the energy-transmitting element by:
(i) applying external contact pressure on the energy-transmitting element;
(ii) applying positive pressure in a lysis container with the sample to be subjected to lysis;
(iii) combined application of positive pressure around a lysis container with the sample to be subjected to lysis and a counteracting force, applied from the outside, on the energy-transmitting element; or
(iv) applying negative pressure around the energy-transmitting element.

9. The device according to claim 1 , wherein the holding element consists of a polymer or of elastomer.

10. The device according to claim 1 , wherein said energy-transmitting element is configured to transmit energy directly to the sample within the lysis container.

11. A system, comprising:
an energy-transmitting element, wherein the energy-transmitting element is a sonotrode or a horn; and
a device configured to lyse a sample to be subjected to lysis, the device including;
a lysis container for containing the sample to be subjected to lysis, the container having a cover to enclose the sample within the container; and
a holding element configured to hold an energy-transmitting element such that the holding element lies around a held part of the energy-transmitting element and envelops the held part of the energy-transmitting element,
the holding element further incorporated into one or more of the lysis container and the cover for the lysis container so that the holding element is to be at least partially immersed into the sample to be subjected to lysis.

12. The device according to claim 1 , wherein the holding element is configured to hold at least part of the energy-transmitting element.

13. The system according to claim 11 , wherein the device is a lab-on-a-chip configured to be placed in contact with the sample to be subjected to lysis.

14. The system according to claim 11 , wherein said energy-transmitting element is configured to transmit energy directly to the sample within the lysis container.

Assignees

• Bosch Gmbh Robert

Inventors

• Daub Martina
• Rupp Jochen
• Rothacher Peter

Classifications

• C12M45/02

  by mechanical forces; Stirring; Trituration; Comminuting (crushing, pulverizing, disintegrating in general B02C) · CPC title

• C12N1/06

  Lysis of microorganisms · CPC title

• C12M47/06

  Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material (lysis of microorganisms C12N1/06; extracting or separating nucleic acids from biological samples C12N15/1003) · CPC title

• C12N13/00 Primary

  Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves · CPC title

• C12N1/066

  by physical processes · CPC title