Construct for promoting absorption of molecules by a cell and methods of using the construct

Patent metadata

Field	Value
Publication number	US-9815921-B2
Application number	US-201213602616-A
Country	US
Kind code	B2
Filing date	Sep 4, 2012
Priority date	Sep 1, 2011
Publication date	Nov 14, 2017
Grant date	Nov 14, 2017

Abstract

The present invention is directed to a construct for promoting absorption of molecules by a cell and the application thereof in drug and gene delivery. The present invention further describes topographical modulation of endocytosis for drug and gene delivery.

First claim

The invention claimed is:

1. A construct for promoting absorption of molecules by a cell located at the surface of the construct; wherein the construct comprises:
a plurality of protrusions located at the surface of the construct;
wherein the protrusions are in the form of pillars having a diameter of between about 200 nm to about 2 μm;
wherein the pitch between the protrusion from edge to edge is between 150 to 300 nm such that the pitch between the protrusions is less than the size of the cells to be located at the surface of the construct;
wherein the protrusions have a size and are spaced apart from each other at a distance that promotes absorption of molecules by said cell and wherein the absorption of molecules is facilitated by endocytosis, or receptor mediated endocytosis, or pinocytosis, or phagocytosis;
wherein the pillars are collapsed pillars lying at the surface of the construct; and
wherein the collapsed pillars have a length of between about 50 nm to about 5 μm.

2. The construct of claim 1 , wherein the protrusions are arranged in an isotropic pattern.

3. The construct of claim 1 , wherein the protrusions are arranged in an anisotropic pattern.

4. The construct of claim 1 , wherein the protrusions are located at the surface of the construct in a detachable form.

5. The construct of claim 1 , wherein the protrusions are located at the surface of the construct in a non-detachable form.

6. The construct of claim 1 , wherein the protrusions are round.

7. The construct of claim 1 , wherein the protrusions are polygonal.

8. The construct of claim 1 , wherein the pillars are extend about 50 nm to 4 μm, or about 100 nm to about 2 μm above the surface of the construct.

9. The construct of claim 1 , wherein the pitch between the pillars from edge to edge is between 200 nm to 250 nm.

10. The construct of claim 1 , wherein a residual layer is arranged between the pillars and the surface of the construct.

11. The construct of claim 1 , wherein the construct is obtained via nano-imprinting lithography.

12. The construct of claim 11 , wherein the nano-imprinting lithography is thermal nano-imprinting lithography or UV nano-imprinting lithography.

13. The construct of claim 11 , wherein the construct and/or the protrusions are made of a polymer.

14. The construct of claim 13 , wherein the polymer is a synthetic polymer, or a rigid synthetic polymer, or a bioresorbable polymer, or a biodegradable polymer.

15. The construct of claim 14 , wherein the synthetic polymer is selected from the group consisting of poly(methyl methacrylate) (PMMA), polydimethylsiloxane (PDMS), polystyrene (PS) and mixtures thereof.

16. The construct of claim 15 , wherein the polystyrene is tissue-culture grade polystyrene (TCPS).

17. The construct of claim 14 , wherein the biodegradable polymer is selected from the group consisting of chitosan, poly(ε-caprolactone), polyglycolic acid, poly(lactic acid), polyphosphoester (PPE) and mixtures thereof.

18. The construct of claim 14 , wherein the rigid synthetic polymer is polystyrene.

19. The construct of claim 13 , wherein during manufacture the polymer is mixed with molecules which are to be absorbed by the cell to be located on the construct.

20. The construct of claim 13 , wherein the molecules are attached to the surface of the protrusions.

Assignees

• Yim King Fai Evelyn
• Low Hong Yee
• Kustandi Tanu Suryadi
• Teo Kim Kiat
• Goh Seok Hong
• Agency For Science Tech And Research (A*Star)
• Nat Univ Singapore

Inventors

• Yim King Fai Evelyn
• Low Hong Yee
• Kustandi Tanu Suryadi
• Teo Kim Kiat
• Goh Seok Hong

Classifications

• Y10T428/24355

  Continuous and nonuniform or irregular surface on layer or component [e.g., roofing, etc.] · CPC title

• G03F7/0002 Primary

  Lithographic processes using patterning methods other than those involving the exposure to radiation, e.g. by stamping · CPC title

• C08F112/08 Primary

  Styrene · CPC title

• C12N5/0068

  General culture methods using substrates (for specific animal cell type C12N5/06) · CPC title

• C12N2533/30

  Synthetic polymers · CPC title