Polymers and DNA copolymer coatings

Patent metadata

Field	Value
Publication number	US-9815916-B2
Application number	US-201514927252-A
Country	US
Kind code	B2
Filing date	Oct 29, 2015
Priority date	Oct 31, 2014
Publication date	Nov 14, 2017
Grant date	Nov 14, 2017

Abstract

Some embodiments described herein relate to new polymer coatings for surface functionalization and new processes for grafting pre-grafted DNA-copolymers to surface(s) of substrates for use in DNA sequencing and other diagnostic applications.

First claim

What is claimed is:

1. A polymer for surface functionalization, comprising a recurring unit of Formula (I) and a recurring unit of Formula (II):

wherein:
each R 1a , R 2a , R 1b and R 2b is independently selected from the group consisting of hydrogen, optionally substituted alkyl, and optionally substituted phenyl;
each R 3a and R 3b is independently selected from the group consisting of hydrogen, optionally substituted alkyl, optionally substituted phenyl, and optionally substituted C 7-14 aralkyl; and
each L 1 and L 2 is independently selected from the group consisting of an optionally substituted alkylene linker and an optionally substituted heteroalkylene linker.

2. The polymer of claim 1 , wherein R 1a is hydrogen or optionally substituted alkyl and each of R 2a and R 3a is hydrogen.

3. The polymer of claim 1 , wherein R 1b is hydrogen or optionally substituted alkyl and each of R 2b and R 3b is hydrogen.

4. The polymer of claim 1 , wherein L 2 is an optionally substituted alkylene.

5. The polymer of claim 1 , wherein L 1 is an optionally substituted alkylene.

6. The polymer of claim 1 , wherein the recurring unit of Formula (I) is represented by Formula (Ia) and Formula (II) is represented by Formula (IIa):

wherein R 1a is hydrogen or methyl and R 1b is hydrogen or methyl.

7. The polymer of claim 1 , wherein L 1 is an optionally substituted heteroalkylene.

8. The polymer of claim 7 , wherein L 1 is —(CH 2 ) m —NH—(CH 2 ) n — optionally substituted with one or more oxo, and wherein each m and n is independently an integer from 1 to 10.

9. The polymer of claim 1 , wherein the recurring unit of Formula (I) is represented by Formula (Ib):

wherein R 1a is hydrogen or methyl.

10. The polymer of claim 1 , further comprising one or more recurring units selected from the group consisting of polyacrylamides, polyacrylates, polyurethanes, polysiloxanes, silicones, polyacroleins, polyphosphazenes, polyisocyanates, poly-ols, and polysaccharides, and combinations thereof.

11. The polymer of claim 10 , further comprising one or more recurring units of Formula (IIIa) or (IIIb) or both:

wherein each R 4a , R 4b , and R 5b is independently hydrogen or C 1-3 alkyl; and
each R 5a , R 6a , R 6b , and R 7b is independently hydrogen, optionally substituted C 1-6 alkyl, or optionally substituted phenyl.

12. The polymer of claim 11 , wherein each R 4a , R 4b , and R 5b is independently hydrogen or methyl.

13. The polymer of claim 11 , wherein R 6b and R 7b are both hydrogen.

14. The polymer of claim 11 , wherein at least one of R 5a or R 6a is hydrogen.

15. The polymer of claim 11 , wherein at least one of R 5a or R 6a is methyl.

16. The polymer of claim 11 , wherein the one or more recurring units of Formula (IIIa) are represented by (IIIa1), (IIIa2), or (IIIa3):

17. The polymer of claim 11 , wherein the one or more recurring units of Formula (IIIb) are represented by (IIIb1):

18. A substrate having a first surface comprising a polymer of claim 1 covalently bonded thereto.

Assignees

• Illumina Cambridge Ltd

Inventors

• Brown Andrew A
• George Wayne N
• Richez Alexandre
• Dingwall Anne-Cecile
• Von Hatten Xavier

Classifications

• C08F220/346

  and further oxygen · CPC title

• C08F220/282

  and containing two or more oxygen atoms · CPC title

• C08F220/56 Primary

  Acrylamide; Methacrylamide · CPC title

• C40B50/18

  using a particular method of attachment to the solid support · CPC title

• C12Q1/6874

  involving nucleic acid arrays, e.g. sequencing by hybridisation · CPC title