Method and compositions for improving selective catabolysis and viability in cells of keratin surfaces

Patent metadata

Field	Value
Publication number	US-9814665-B2
Application number	US-201514753748-A
Country	US
Kind code	B2
Filing date	Jun 29, 2015
Priority date	Jan 7, 2013
Publication date	Nov 14, 2017
Grant date	Nov 14, 2017

Abstract

A method for increasing cellular viability in cells of keratin surfaces after exposure to UV light and/or detoxifying cells of debris that impedes healthy metabolic function by topically applying to the keratin surface a composition containing at least one autophagy activator and at least one DNA repair enzyme and a method for making a topical composition comprising the steps of (a) identifying an ingredient that activates cellular autophagy in keratinocyte cultures in vitro, and (b) formulating the ingredient into a topical composition in combination with at least one DNA repair enzyme.

First claim

We claim:

1. A method for making a topical composition comprising the steps of:
(a) identifying an extract from the Candida genus that activates cellular autophagy in skin cell cultures in vitro by:
(i) measuring gene expression of mTOR in the cultured skin cells;
(ii) selecting the extract that shows an inhibition in mTOR gene expression in the cultured skin cells;

(b) identifying at least one DNA repair enzyme;
(c) combining the extract of (a) and the DNA repair enzyme of (b) and testing on skin cells in vitro by measuring survival after exposure to UV light at 10 J/cm 2 ;
(d) selecting the combination of (c) in amounts that provide a synergistic increase in cellular health and viability when tested on skin cells in vitro; and
(e) adding the selected combination of (d) into a topical composition, wherein the at least one DNA repair enzyme is a base excision repair (BER) enzyme.

2. The method of claim 1 wherein the skin cells are keratinocytes.

3. The method of claim 1 wherein the gene expression measurement is measured by immunofluorescence.

4. The method of claim 1 wherein the gene expression is measured by western blot analysis of phosphorylated proteins associated with the expressed gene.

5. The method of claim 1 wherein the topical composition is in the form of an emulsion prepared by emulsifying oil into a water continuous phase.

6. The method of claim 5 wherein the composition further comprises at least one CLOCK or PER1 gene activator, at least one proteasome activator, or mixtures thereof.

7. The method of claim 1 , wherein the BER enzyme is 8-oxoguanine DNA glycosylase (OGG1).

8. The method of claim 1 wherein the extract from the Candida genus is Candida saitoana.

Assignees

• Elc Man Llc

Inventors

• Pernodet Nadine A
• Collins Donald F
• Layman Dawn
• Yarosh Daniel B

Classifications

• A61P43/00

  Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title

• A61P17/00

  Drugs for dermatological disorders · CPC title

• A61K8/9717

  Rhodophycota or Rhodophyta [red algae], e.g. Porphyra · CPC title

• A61K8/645

  Proteins of vegetable origin; Derivatives or degradation products thereof · CPC title

• A61K8/66 Primary

  Enzymes · CPC title