Recombinant expression vector applicable to rapid screening for recombinant strain and application
US-12037632-B2 · Jul 16, 2024 · US
Method for producing archaeal protein
US9809825B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9809825-B2 |
| Application number | US-201615288203-A |
| Country | US |
| Kind code | B2 |
| Filing date | Oct 7, 2016 |
| Priority date | Apr 10, 2014 |
| Publication date | Nov 7, 2017 |
| Grant date | Nov 7, 2017 |
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Abstract
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An object of the present invention is to provide a means for producing an archaeal protein by extracellular secretion. The system for secretory production of an archaeal protein uses a fungus belonging to the genus Talaromyces as a host.
First claim
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The invention claimed is: 1. A method of producing an archaeal protein, comprising a step of cultivating a fungus belonging to the genus Talaromyces, wherein the fungus has been transformed with an expression vector comprising: a promoter region that functions in the fungus; a secretion signal region located downstream of the promoter region, the secretion signal region encoding a secretion signal peptide of a cellobiohydrolase gene from the genus Talaromyces; a coding region for the archaeal protein located downstream of the secretion signal region; and a terminator region located downstream of the coding region, and wherein the fungus secretes the archaeal protein. 2. The method according to claim 1 , wherein the secretion signal region has the base sequence of SEQ ID NO: 5. 3. The method according to claim 1 , wherein both the promoter region and the terminator region are of glucoamylase from Talaromyces cellulolyticus. 4. The method according to claim 1 , wherein the codon usage in the coding region is optimized for expression in the fungus. 5. The method according to claim 1 , wherein the archaeal protein is a cellulase from the genus Pyrococcus. 6. The method according to claim 1 , wherein the coding region has the base sequence of SEQ ID NO: 6 or 7. 7. The method according to claim 2 , wherein both the promoter region and the terminator region are of glucoamylase from Talaromyces cellulolyticus. 8. The method according to claim 2 , wherein the codon usage in the coding region is optimized for expression in the fungus. 9. The method according to claim 3 , wherein the codon usage in the coding region is optimized for expression in the fungus. 10. The method according to claim 2 , wherein the archaeal protein is a cellulase from the genus Pyrococcus. 11. The method according to claim 3 , wherein the archaeal protein is a cellulase from the genus Pyrococcus. 12. The method according to claim 4 , wherein the archaeal protein is a cellulase from the genus Pyrococcus. 13. The method according to claim 2 , wherein the coding region has the base sequence of SEQ ID NO: 6 or 7. 14. The method according to claim 3 , wherein the coding region has the base sequence of SEQ ID NO: 6 or 7. 15. The method according to claim 4 , wherein the coding region has the base sequence of SEQ ID NO: 6 or 7. 16. The method according to claim 5 , wherein the coding region has the base sequence of SEQ ID NO: 6 or 7.
Assignees
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Classifications
- C12N15/80Primary
for fungi · CPC title
Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase · CPC title
Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150) · CPC title
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Frequently asked questions
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- What does patent US9809825B2 cover?
- An object of the present invention is to provide a means for producing an archaeal protein by extracellular secretion. The system for secretory production of an archaeal protein uses a fungus belonging to the genus Talaromyces as a host.
- Who is the assignee on this patent?
- Nat Inst Advanced Ind Science & Tech
- What technology area does this patent fall under?
- Primary CPC classification C12N15/80. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Nov 07 2017 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).