Galvanotaxis assay for quantitative assessment of the metastatic potential of cancer cells

Patent metadata

Field	Value
Publication number	US-9809810-B2
Application number	US-201414765993-A
Country	US
Kind code	B2
Filing date	Feb 5, 2014
Priority date	Feb 5, 2013
Publication date	Nov 7, 2017
Grant date	Nov 7, 2017

Abstract

An apparatus and method for accelerating and/or inhibiting the migration of cells by applying a time-varying magnetic field to induce eddy currents that promote electrotaxis (galvanotaxis) of cells without the need for chemokines or glucose. The present invention can also be used to study and quantify the metastatic potential of different cell lines.

First claim

What is claimed is:

1. A method for controlling cell migration comprising the steps of:
providing an electromagnetic coil having a first end and a second end;
connecting the electromagnetic coil to a function generator;
applying a time-varying voltage waveform to the electromagnetic coil;
inducing a time-varying electric field around the electromagnetic coil;
placing the electromagnetic coil adjacent to, and without contacting, the location of cancer cells;
directing the induced electric field along an axis of migration of the cancer cells;
orientating the placement of the electromagnetic coil to control the direction of the induced electric field; and
reducing the migration of the cancer cells using the induced electric field.

2. A method according to claim 1 further comprising the step of:
inducing eddy currents near the location of the cancer cells.

3. A method according to claim 1 , wherein the time-varying waveform is a sawtooth waveform.

4. A method according to claim 1 , wherein the time-varying waveform is a 20 volts peak to peak, 100 kHz sawtooth waveform with a 50 ns drop off at its trailing edge.

5. A method according to claim 4 , wherein the sawtooth waveform has a sharp drop and wherein the induced electric field is a rapidly time-varying magnetic field.

6. A method for controlling cell migration comprising the steps of:
providing an electromagnetic coil having a first end and a second end;
connecting the electromagnetic coil to a function generator;
applying a time-varying sawtooth voltage waveform to the electromagnetic coil;
inducing a time-varying electric field around the electromagnetic coil;
placing the electromagnetic coil adjacent to, and without contacting, the location of cancer cells;
directing the induced electric field along an axis of migration of the cancer cells, and wherein the induced electric field switches direction along the axis of migration with a non-equal component directed towards or away from a direction of migration;
orientating the placement of the electromagnetic coil to control the direction of the nonequal component of the induced electric field; and
reducing migration of the cancer cells using the induced electric field.

7. A method according to claim 6 , wherein the time-varying waveform is a 20 volts peak to peak, 100 kHz sawtooth waveform with a 50 ns drop off at its trailing edge.

8. A method according to claim 6 , wherein the sawtooth waveform has a sharp drop and wherein the induced electric field is a rapidly time-varying magnetic field.

9. A method according to claim 6 , further comprising the steps of:
placing the electromagnetic coil in between a first row of a plurality of assay wells and second row of a plurality of assay wells;
providing a plurality of well inserts having a porous membrane;
placing one of the well inserts into each of the plurality of assay wells so that the wells are divided into a lower and upper compartment;
placing a medium into each of the plurality of assay wells;
placing a predetermined line of cancer cells into each of the assay wells; and
allowing the predetermined lines of cancer cells to settle on top of the porous membranes.

10. A method according to claim 1 , wherein the induced electric field switches direction along the axis of migration with a non-equal component directed towards or away from a direction of migration; and further comprising the step of:
orientating the placement of the electromagnetic coil to control the direction of the nonequal component of the induced electric field.

Assignees

• Ohio State Innovation Foundation

Inventors

• Subramaniam Vish
• West Joseph
• Alkandry Emily
• Nasser Mohd
• Ahirwar Dinesh
• Ganju Ramesh

Classifications

• A61N2/02

  using magnetic fields produced by coils, including single turn loops or electromagnets (A61N2/12 takes precedence) · CPC title

• C12M25/04

  in combination with well or multiwell plates, i.e. culture inserts · CPC title

• C12N13/00 Primary

  Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves · CPC title

• A61N2/004 Primary

  specially adapted for a specific therapy · CPC title

• C12M23/12

  Well or multiwell plates (C12M25/04 takes precedence) · CPC title