Apparatus and method for separating and concentrating a component of a fluid

Patent metadata

Field	Value
Publication number	US-9808568-B2
Application number	US-201514803440-A
Country	US
Kind code	B2
Filing date	Jul 20, 2015
Priority date	Mar 29, 2012
Publication date	Nov 7, 2017
Grant date	Nov 7, 2017

Abstract

A system for separating and concentrating a component of a whole material is disclosed. The whole material can include a material that has more than one component, such as whole blood that can include at least red blood cells, monocytes, and plasma. The system can include a substantially single container including a separation section and a concentration section wherein a component can be moved from the separation section, after a separation, to the concentration section to be concentrated. The concentrated component can then be withdrawn from the separation and concentration container for a selected procedure.

First claim

What invention claimed is:

1. A method to separate and concentrate a component of a whole material, comprising:
placing a whole material in a separation section of an integrated separation and concentration container;
applying a first force to the integrated separation and concentration container to cause separation of the whole material such that at least a portion of the whole material moves towards a first terminal end of the integrated separation and concentration container;
withdrawing the component from a buoy separation volume of a buoy system within the separation section;
placing the withdrawn component into a concentration section of the integrated separation and concentration container;
mixing the component in the concentration section with an activating/concentrating material; and
applying a second force to the integrated separation and concentration container to remove at least a portion of the component from the mixture of the activating/concentrating material.

2. The method of claim 1 , further comprising:
withdrawing the removed portion of the component from the mixture of the activating/concentrating material from the a concentration section of the integrated separation and concentration container.

3. The method of claim 1 , wherein the mixing the component in the concentration section with an activating/concentrating material causes a concentration of a selected portion of the component mixed with the activating/concentrating material such that the removed portion of the component is concentrated above a concentration of the selected component.

4. The method of claim 1 , wherein mixing the component in the concentration section with the activating/concentrating material includes mixing the component with a polyacrylamide material.

5. The method of claim 1 , wherein mixing the component in the concentration section with the activating/concentrating material includes releasing a selected protein from the component; and
wherein applying the second force to the separation and concentration container to remove at least a portion of the component from the mixture of the activating/concentrating material includes moving the selected protein to a withdrawal portion of the concentration section.

6. The method of claim 2 , further comprising:
obtaining the volume of whole material from a patient; and
applying the withdrawn removed at least a portion of the component to the patient.

7. The method of claim 1 , wherein the first force is different than the second force.

8. The method of claim 1 , further comprising:
moving a plunger of a separation syringe in a first direction to apply the third force to the integrated separation and concentration container and draw the removed portion of the component into the syringe.

9. The method of claim 8 , further comprising:
moving the plunger in a second direction to apply a fourth force to the removed portion in the separation syringe to place the removed portion into the concentration section;
wherein the first direction is opposite to the second direction.

10. The method of claim 9 , wherein a check valve diverts the removed portion into the concentration section when the removed portion is forced from the separation syringe.

11. The method of claim 1 , further comprising:
agitating the integrated separation and concentration container when the withdrawn component is placed in the concentration section.

12. The method of claim 1 , further comprising:
filtering the removed portion of the component to prevent removing the activating/concentrating material from the concentration section.

13. The method of claim 12 , further comprising:
applying a fifth force to the integrated separation and concentration container to force the removed portion through a filter.

14. The method of claim 13 , further comprising:
collecting the filtered removed portion in a sump for removal from the integrated separation and concentration container.

15. The method of claim 1 , further comprising:
moving a plunger of a withdrawal syringe in a first direction to apply the second force to the integrated separation and concentration container to withdraw the removed portion.

Assignees

• Biomet Biologics Llc

Inventors

• O'Connell Jr Patrick S

Classifications

• A61M39/08

  Tubes; Storage means specially adapted therefor · CPC title

• B01L3/502

  with fluid transport, e.g. in multi-compartment structures · CPC title

• B01L2300/0681

  Filter · CPC title

• G01N33/491 Primary

  by separating the blood components (G01N15/05 takes precedence) · CPC title

• B01L2300/046

  Function or devices integrated in the closure · CPC title