Integrated spectral probe for raman, reflectance and fluorescence spectral measurements

Patent metadata

Field	Value
Publication number	US-9804092-B2
Application number	US-201414766451-A
Country	US
Kind code	B2
Filing date	Feb 11, 2014
Priority date	Feb 14, 2013
Publication date	Oct 31, 2017
Grant date	Oct 31, 2017

Abstract

Examples of a spectroscopy probe for performing measurements of Raman spectra, reflectance spectra and fluorescence spectra are disclosed. The integrated spectral probe can comprise one or more light sources to provide a white light illumination to generate reflectance spectra, an excitation light to generate an UV/visible fluorescence spectra and a narrow band NIR excitation to induce Raman spectra. The multiple modalities of spectral measurements can be performed within 2 seconds or less. Examples of methods of operating the integrated spectroscopy probe disclosed.

First claim

The invention claimed is:

1. An integrated spectroscopy system to facilitate integrated measurements of Raman spectra, reflectance spectra and fluorescence spectra, the system comprising:
a light source configured to provide a white light illumination beam to generate reflectance spectra, an excitation light beam to generate an UV/visible fluorescence spectra and a narrow band excitation light beam to induce Raman spectra;
an integrated spectroscopy probe coupled to the light source, the spectroscopy probe comprising:
at least one narrow band excitation fiber configured to transmit the narrow band excitation light beam to a target;
at least one white light illumination fiber configured to transmit the white light illumination beam to the target;
at least one excitation illumination fiber configured to transmit the excitation light beam to the target;
a plurality of Raman collection fibers configured to collect a returning radiation reflected and emitted from the target in response to the narrow band excitation light beam;
at least one reflectance collection fiber configured to collect a returning radiation reflected from the target in response to the white light illumination beam; and

at least one fluorescence collection fiber configured to collect returned radiation emitted from the target in response to the excitation light beam;
a first detector coupled to the plurality of Raman collection fibers to detect a Raman spectrum; and
at least one additional detector coupled to the at least one reflectance collection fibers and/or the at least one fluorescence collection fibers to detect a reflectance spectrum and/or a fluorescence spectrum,
wherein the at least one white light illumination fiber, the at least one excitation illumination fiber, the at least one reflectance collection fiber and the at least one fluorescence collection fiber are placed between the at least one narrow band excitation fiber and the plurality of Raman collection fibers and are each spaced apart from the at least one narrow band excitation fiber and the plurality of Raman collection fibers.

2. The integrated spectroscopy system of claim 1 , wherein pluralities of spectral measurements are being performed within 2 seconds or less.

3. The integrated spectroscopy system of claim 1 , further comprise an optical modulator mounted in front of the light source configured to modulate the illumination light from the light source and provide the white light illumination light beam, the excitation light beam and the narrow band excitation beam.

4. The integrated spectroscopy system of claim 3 , wherein the optical modulator comprises a first filter configured to pass visible light below 750 nm, a second filter configured to pass excitation light in a blue wavelength range and a third filter configured to pass light in a 785±5 nm wavelength range.

5. The integrated spectroscopy system of claim 1 , wherein the light source comprises a monochromatic light source configured to provide the narrow band excitation light beam, a broadband light source configured to provide white light illumination light beam and a excitation light source configured to provide the excitation light beam, the monochromatic light source being coupled to the at least one narrow band excitation fiber, the broadband light source being coupled to the at least one white light illumination fiber, the excitation light source being coupled to the at least one excitation illumination fiber.

6. The integrated spectroscopy system of claim 5 , wherein the broadband light source and the excitation light source are integrated into one light source, the integrated light source is configured to provide a white light illumination light beam and the excitation light beam in sequence.

7. The integrated spectroscopy system of claim 1 , wherein the at least one narrow band excitation fiber is spaced apart from the plurality of Raman collection fibers; the at least one white light illumination fiber is spaced apart from the at least one reflectance collection fiber; and the at least one excitation illumination fiber is spaced apart from the at least one fluorescence collection fiber.

8. The integrated spectroscopy system of claim 1 , further comprises a filter assembly mounted at a distal end of the spectroscopy probe, the filter assembly comprising a short pass filter placed over the at least one narrow band excitation fiber to pass light in a 785±5 nm wavelength range and a long pass filter placed over the plurality of Raman collection fibers to block light in a 785 nm wavelength range and pass light in a wavelength range above 800 nm.

9. The integrated spectroscopy system of claim 8 , wherein the filter assembly being glued to the distal end of the probe, the probe further comprising a grooved channel formed at the distal end configured to capture any excess glue.

10. The integrated spectroscopy system of claim 1 , wherein the probe further comprises a filter mounted into the probe at a proximal end of the probe, the filter being coupled to the at least one narrow band excitation fiber to pass light in a 785±5 nm wavelength range.

11. The integrated spectroscopy system of claim 1 , wherein the at least one additional detector is a compact spectrometer.

12. The integrated spectroscopy system of claim 1 , further comprising a control system in optical communication with the light source to control the output illumination of the light source.

13. The integrated spectroscopy system of claim 12 , wherein the control system being in optical communication with the first detector and the at least one additional detector, the control system configured to control the operation of the first detector and the at least one additional detector.

14. The integrated spectroscopy system as in one of claims 1 - 13 , wherein the light source provides simultaneously the white light illumination beam and the narrow band excitation beam such that the first detector and the one additional detector provide simultaneously the Raman spectrum and the reflectance spectrum.

15. The integrated spectroscopy system as in one of claims 1 to 13 , wherein the light source provides simultaneously the excitation light beam and the narrow band excitation light beam such that the first detector and the one additional detector provide simultaneously the Raman spectrum and the fluorescence spectrum.

16. The integrated spectroscopy spectrum as in one of claims 1 to 13 , wherein the light source is configured to provide the narrow band illumination light beam to generate simultaneously a Raman spectrum and an infrared fluorescence spectrum such that the first detector being configured to provide simultaneously the Raman spectrum and the infrared fluorescence spectrum.

Assignees

• British Columbia Cancer Agency Branch

Inventors

• Zeng Haishan
• Short Michael
• Zhao Jianhua

Classifications

• G01N21/645

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• G01N2201/084

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• G01J3/0221

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• G01N2021/6417

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