Detecting neoplasm

Patent metadata

Field	Value
Publication number	US-9803249-B2
Application number	US-201715467739-A
Country	US
Kind code	B2
Filing date	Mar 23, 2017
Priority date	Feb 15, 2008
Publication date	Oct 31, 2017
Grant date	Oct 31, 2017

Abstract

This document relates to methods and materials for detecting premalignant and malignant neoplasms. For example, methods and materials for determining whether or not a stool sample from a mammal contains nucleic acid markers or polypeptide markers of a neoplasm are provided.

First claim

What is claimed is:

1. A method for characterizing a human biological sample comprising:
(a) measuring a K-ras mutation score from a human biological sample, wherein said measuring of said K-ras mutation score is measured by digital melt curve analysis;
(b) measuring a methylation level of a CpG site of from the human biological sample;
(c) determining whether or not the human biological sample has an elevated K-ras mutation score and an elevated BMP3 methylation status as compared to a control from a subject that does not have premalignant or malignant colorectal cancer.

2. The method of claim 1 , wherein the human biological sample is a stool sample.

3. A method for characterizing a human stool sample comprising:
(a) measuring a K-ras mutation score from a human stool sample;
(b) measuring a methylation level of a CpG site of BMP3 from the human stool sample;
(c) determining whether or not the stool biological sample has an elevated K-ras mutation score and an elevated BMP3 methylation status as compared to a control from a subject that does not have premalignant or malignant colorectal cancer.

4. The method of claim 3 , wherein said measuring of said K-ras mutation score is measured by digital melt curve analysis.

5. The method of claim 3 , wherein said measuring of said K-ras mutation score is measured by quantitative allele-specific PCR.

Assignees

• Mayo Foundation

Inventors

• Taylor William R
• Harrington Jonathan J
• Quint Patrick S
• Zou Hongzhi
• Bergen Iii Harold S
• Smith David I
• Ahlquist David A

Classifications

• C12Q1/6886 Primary

  for cancer (immunoassay for cancer G01N33/575) · CPC title

• G01N2333/928

  acting on alpha -1, 4-glucosidic bonds, e.g. hyaluronidase, invertase, amylase · CPC title

• G01N2333/966

  Elastase · CPC title

• C12Q1/40

  involving amylase · CPC title

• C12Q2600/156

  Polymorphic or mutational markers · CPC title