Primers and probes for detecting human papillomavirus and human beta globin sequences in test samples

Patent metadata

Field	Value
Publication number	US-9803232-B2
Application number	US-201514744115-A
Country	US
Kind code	B2
Filing date	Jun 19, 2015
Priority date	Sep 30, 2008
Publication date	Oct 31, 2017
Grant date	Oct 31, 2017

Abstract

The present invention relates to primers, probes, primer sets, primer and probe sets, methods and kits for detecting human papillomaviruses, human beta globin sequences and human papillomaviruses and human beta globin sequences in a test sample.

First claim

What is claimed is:

1. A primer and probe kit for detecting human beta globin in a test sample, wherein said kit comprises:
(a) a forward primer consisting of SEQ ID NO:6 or the complement thereof;
(b) a reverse primer consisting of SEQ ID NO:7 or the complement thereof;
(c) a probe consisting of SEQ ID NO:22 or the complement thereof and at least one fluorescent reporter moiety;
(d) three forward primers each consisting of a sequence selected from SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, and the complements thereof and two reverse primers each consisting of a sequence selected from SEQ ID NO:4, SEQ ID NO:5, and the complements thereof, and
(e) fourteen probes, wherein each probe consists of a sequence selected from SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, and the complements thereof.

2. A method for detecting human beta globin in a test sample, the method comprising the steps of:
contacting the test sample with the primers from the primer and probe kit of claim 1 under amplification conditions to generate a first target sequence; and
detecting hybridization between the first target sequence and the probe from the primer and probe kit of claim 1 as an indication of the presence of a human beta globin in the test sample.

3. The method of claim 2 , wherein the probe is labeled with a detectable label.

4. The method of claim 3 , wherein the detectable label comprises a fluorescent moiety attached at the 5′ end of the probe.

5. The method of claim 4 , wherein the probe further comprises a quencher moiety attached at its 3′ end.

Assignees

• Abbott Molecular Inc

Inventors

• Abravaya Klara
• Erickson Brian J
• Huang Shihai X
• Mak Wai-Bing X
• Salituro John A
• Tang Ning

Classifications

• C12Q2600/112

  Disease subtyping, staging or classification · CPC title

• C12Q2600/16

  Primer sets for multiplex assays · CPC title

• C12Q1/6886 Primary

  for cancer (immunoassay for cancer G01N33/575) · CPC title

• C12Q1/708

  for papilloma · CPC title

• C12Q1/6806 Primary

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title