Infrared signal monitoring for cell cultures

Patent metadata

Field	Value
Publication number	US-9803170-B2
Application number	US-201615188084-A
Country	US
Kind code	B2
Filing date	Jun 21, 2016
Priority date	Dec 10, 2015
Publication date	Oct 31, 2017
Grant date	Oct 31, 2017

Abstract

The present invention is directed to an apparatus for monitoring a cell culture comprising a) one or more infrared sensors positioned adjacent to a cell culture, the one or more infrared sensors capable of recording an infrared heat signal from the cell culture; b) a power source in electrical communication with the one or more infrared sensors; c) a data storage and computation device configured to receive and analyze the infrared heat signal from the one or more infrared sensors; and d) a transmitter device in electrical communication with the storage and computation device; wherein the apparatus monitors the pattern of heat production in the cell culture. The present invention is also directed to methods for monitoring and analyzing the metabolic activity of cells using the above apparatus.

First claim

What is claimed is:

1. An apparatus for monitoring a cell culture, comprising:
one or more high definition infrared sensors positioned adjacent to a cell culture, said one or more infrared sensors capable of recording high definition images of infrared spectrum emission from said cell culture;
a power source in electrical communication with said one or more infrared sensors;
a data storage and computation device configured to receive and analyze said high definition images from said one or more infrared sensors; and
a transmitter device in electrical communication with said storage and computation device;
wherein said apparatus analyzes the high definition images using computerized image recognition to determine at least the amount of heat produced by the cell culture, regions of the cell culture where heat is produced, and a change in heat production over time to provide an analysis;
wherein said apparatus provides a metabolism analysis based upon the analysis, wherein the metabolism analysis comprises a determination of a change in metabolism based upon the change in heat production, wherein an increase in heat production is correlated with an increase in metabolism and a decrease in heat production is correlated with a decrease in metabolism.

2. The apparatus of claim 1 , wherein said one or more infrared sensors comprise one or more infrared cameras.

3. The apparatus of claim 1 , further comprising an amplifier positioned between said one or more infrared sensors and said data storage and computation device.

4. The apparatus of claim 1 , further comprising a control unit in communication with said data storage and computation device.

5. The apparatus of claim 4 , further comprising a reference component in communication with said control unit, said reference component comprising
one or more infrared sensors positioned adjacent to a reference sample, said one or more infrared sensors capable of recording an infrared heat signal from said reference sample;
a power source in electrical communication with said one or more infrared sensors;
a data storage and computation device configured to receive and analyze said infrared heat signal from said one or more infrared sensors; and
a transmitter device in electrical communication with said storage and computation device;
wherein said reference component monitors the pattern of heat production in said reference sample and wherein said control unit compares said pattern of heat production in said cell culture with said pattern of heat production in said reference sample.

6. The apparatus of claim 1 , wherein said cell culture is contained in a culture vessel.

7. The apparatus of claim 6 , wherein said culture vessel is a petri dish.

8. The apparatus of claim 6 , wherein said culture vessel is a flask.

9. The apparatus of claim 6 , wherein said culture vessel is a test tube.

10. The apparatus of claim 6 , wherein said culture vessel is a well plate.

11. The apparatus of claim 6 , wherein said culture vessel comprises infrared transparent materials.

12. The apparatus of claim 1 , wherein said one or more infrared sensors are positioned above said cell culture.

13. The apparatus of claim 1 , further comprising one or more lenses positioned between said one or more infrared sensors and said cell culture.

Assignees

• IBM

Inventors

• Cahan Amos
• Van Kessel Theodore G

Classifications

• C12M41/48

  Automatic or computerized control (automatic analysis G01N35/00) · CPC title

• C12M41/46 Primary

  of cellular or enzymatic activity or functionality, e.g. cell viability · CPC title

• C12M23/22

  Transparent or translucent parts (glassware for laboratory use B01L3/00) · CPC title

• C12M23/08

  Flask, bottle or test tube · CPC title

• C12M23/10

  Petri dish (crystallising dishes B01L3/06) · CPC title